Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HK86_RS00220 Genome accession   NC_021847
Coordinates   39699..40715 (-) Length   338 a.a.
NCBI ID   WP_020839764.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus O1:Kuk str. FDA_R31     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 34699..45715
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HK86_RS00195 (M634_00205) aroK 35559..36077 (-) 519 WP_005381319.1 shikimate kinase AroK -
  HK86_RS00200 (M634_00210) pilQ 36266..38014 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  HK86_RS00205 (M634_00215) pilP 38046..38561 (-) 516 WP_015297395.1 pilus assembly protein PilP Machinery gene
  HK86_RS00210 (M634_00220) pilO 38551..39144 (-) 594 WP_020839762.1 type 4a pilus biogenesis protein PilO Machinery gene
  HK86_RS00215 (M634_00225) pilN 39137..39715 (-) 579 WP_020839763.1 PilN domain-containing protein Machinery gene
  HK86_RS00220 (M634_00230) pilM 39699..40715 (-) 1017 WP_020839764.1 type IV pilus biogenesis protein PilM Machinery gene
  HK86_RS00225 (M634_00235) - 40858..43428 (+) 2571 WP_005496567.1 penicillin-binding protein 1A -
  HK86_RS00230 (M634_00240) oxyR 43485..44393 (-) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  HK86_RS00235 (M634_00245) - 44564..45292 (+) 729 WP_015297397.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37762.28 Da        Isoelectric Point: 6.8322

>NTDB_id=49778 HK86_RS00220 WP_020839764.1 39699..40715(-) (pilM) [Vibrio parahaemolyticus O1:Kuk str. FDA_R31]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPDKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=49778 HK86_RS00220 WP_020839764.1 39699..40715(-) (pilM) [Vibrio parahaemolyticus O1:Kuk str. FDA_R31]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTATTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTCGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACAGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAAGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGTCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGATAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGAACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAACGGTTCGCAAAACATTAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGACGAGCGGCTGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTTTATCGCATTAA

Domains

Predicted by InterProScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.615

100

0.846
  pilM Vibrio cholerae strain A1552

49.263

100

0.494

Multiple sequence alignment