Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   IOD66_RS01560 Genome accession   NZ_CP063424
Coordinates   301918..302466 (+) Length   182 a.a.
NCBI ID   WP_011848557.1    Uniprot ID   A3N1S3
Organism   Actinobacillus pleuropneumoniae strain APP5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 296918..307466
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IOD66_RS01510 (IOD66_01525) - 297170..297337 (+) 168 WP_009875287.1 Trm112 family protein -
  IOD66_RS01515 (IOD66_01530) - 297494..297754 (-) 261 WP_009875288.1 Txe/YoeB family addiction module toxin -
  IOD66_RS01520 (IOD66_01535) yefM 297751..298002 (-) 252 WP_005605062.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -
  IOD66_RS01530 (IOD66_01545) gltX 298340..299779 (-) 1440 WP_009875289.1 glutamate--tRNA ligase -
  IOD66_RS01555 (IOD66_01570) - 300683..301864 (+) 1182 WP_009875290.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  IOD66_RS01560 (IOD66_01575) pilF 301918..302466 (+) 549 WP_011848557.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  IOD66_RS01565 (IOD66_01580) - 302541..304880 (+) 2340 WP_247797616.1 Tex family protein -
  IOD66_RS01570 (IOD66_01585) - 305072..306358 (-) 1287 WP_005615681.1 NAD(P)/FAD-dependent oxidoreductase -
  IOD66_RS01575 (IOD66_01590) - 306463..306984 (-) 522 WP_193327846.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20671.35 Da        Isoelectric Point: 6.9479

>NTDB_id=495556 IOD66_RS01560 WP_011848557.1 301918..302466(+) (pilF) [Actinobacillus pleuropneumoniae strain APP5]
MNLAKFLRNLTACMLLSVLFGCSSQLPTSQTTFNRSEAVKARINLALAYLDQHDLPKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQVHAEQAYQQALKLSDNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVKSEQPYYHQADSLENIVLCAKQEPNL
AKVNEALSQLEKLDKSRAAALR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=495556 IOD66_RS01560 WP_011848557.1 301918..302466(+) (pilF) [Actinobacillus pleuropneumoniae strain APP5]
ATGAACCTTGCAAAATTTTTGCGAAATTTGACCGCTTGTATGCTGTTATCCGTGTTATTCGGTTGTTCTTCTCAGCTTCC
AACCTCCCAAACTACCTTTAATCGTTCGGAAGCGGTTAAAGCCCGTATTAATTTAGCCTTAGCGTATCTCGATCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCGCTAAGCCACGATCAAAACGATTATTTGCCTCACTCTGTTTTAGCT
TATTACTATCAGCAAATCGGTGATCAAGTTCATGCGGAACAGGCTTATCAGCAAGCATTGAAATTAAGTGATAATCGTCC
GGATGTCTTAAATAATTACGGTACTTTTCTGTGTAAACAAGCCAAATTTGATCAGGCATATCAGCAGTTTGAGCAAGCGG
TGAAAAGCGAACAACCTTATTATCATCAAGCCGATAGCCTCGAAAATATTGTGTTATGTGCCAAACAAGAGCCGAATTTA
GCTAAAGTAAACGAAGCGTTAAGCCAATTAGAAAAGCTGGATAAATCAAGAGCGGCGGCACTACGCTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(79-104)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A3N1S3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

67.021

100

0.692
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.784

93.956

0.44