Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   INP70_RS02310 Genome accession   NZ_CP063420
Coordinates   483278..484153 (-) Length   291 a.a.
NCBI ID   WP_000418751.1    Uniprot ID   -
Organism   Vibrio cholerae strain VC_hf7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 478278..489153
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP70_RS02290 parC 479212..481497 (+) 2286 WP_000102510.1 DNA topoisomerase IV subunit A -
  INP70_RS02295 yacG 481546..481743 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  INP70_RS02300 zapD 481901..482641 (-) 741 WP_000207194.1 cell division protein ZapD -
  INP70_RS02305 coaE 482673..483281 (-) 609 WP_000011556.1 dephospho-CoA kinase -
  INP70_RS02310 pilD 483278..484153 (-) 876 WP_000418751.1 A24 family peptidase Machinery gene
  INP70_RS02315 pilC 484211..485437 (-) 1227 WP_000648396.1 type II secretion system F family protein Machinery gene
  INP70_RS02320 pilB 485480..487168 (-) 1689 WP_000957207.1 type IV-A pilus assembly ATPase PilB Machinery gene
  INP70_RS02325 - 487175..487585 (-) 411 WP_000649335.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  INP70_RS02330 nadC 487830..488720 (-) 891 WP_000665277.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32447.72 Da        Isoelectric Point: 7.1618

>NTDB_id=495515 INP70_RS02310 WP_000418751.1 483278..484153(-) (pilD) [Vibrio cholerae strain VC_hf7]
MELFYFYPWLFPVLATLFGLIVGSFLNVVIYRLPKIMEREWRAECAASFPEYGITPPEGKLTLSLPRSTCPHCQTPIRVI
DNIPLLSWLALRGQCSHCKAPISARYPLIELLTALMSLVIATHFPFGVFAVALLFFTYVLIAATFIDFDTLLLPDQLTLP
LLWGGIALALLGFSPVSLSDAVIGAMAGYLSLWSIYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPVIVLLSSVVGV
IFGLIQLRQQKKGIDMAFPFGPYLAIAGWFALLWGDKVIDWYFTTWVGQPL

Nucleotide


Download         Length: 876 bp        

>NTDB_id=495515 INP70_RS02310 WP_000418751.1 483278..484153(-) (pilD) [Vibrio cholerae strain VC_hf7]
ATGGAACTGTTTTACTTCTACCCTTGGCTGTTTCCGGTGCTCGCCACCCTGTTTGGCTTGATTGTCGGTAGCTTTCTCAA
TGTGGTGATCTACCGCTTACCCAAAATCATGGAGCGTGAATGGCGCGCGGAATGTGCGGCGAGTTTTCCTGAATATGGCA
TCACGCCACCAGAGGGTAAACTGACCCTCAGTTTGCCGCGTTCCACTTGCCCACACTGCCAAACGCCGATTCGCGTCATT
GATAATATCCCGCTACTGAGTTGGCTGGCGCTGCGCGGTCAATGCTCACACTGTAAAGCGCCGATCAGCGCTCGCTATCC
CTTGATTGAGCTACTCACTGCCCTCATGAGCTTAGTGATTGCTACTCACTTTCCGTTCGGGGTGTTTGCAGTGGCGCTGC
TGTTTTTCACCTATGTATTGATTGCGGCGACCTTCATCGACTTCGATACCCTGTTGCTGCCCGACCAATTAACGCTACCT
TTACTGTGGGGCGGTATTGCACTGGCACTGCTTGGTTTTTCTCCGGTTTCACTCAGTGATGCGGTGATCGGAGCCATGGC
GGGTTATCTCTCGCTGTGGTCGATTTACTGGCTATTTAAACTACTGACAGGTAAAGAAGGCATGGGTTATGGCGATTTCA
AATTGCTCGCCGCACTCGGCGCTTGGCTGGGTTGGCAGCAGTTGCCTGTCATCGTACTGCTCTCATCGGTGGTCGGGGTG
ATTTTCGGTTTAATCCAACTGCGCCAGCAGAAAAAAGGTATCGATATGGCCTTTCCGTTTGGCCCTTATCTCGCCATCGC
GGGGTGGTTTGCCTTGCTGTGGGGCGATAAGGTAATTGATTGGTACTTCACCACTTGGGTAGGACAACCGTTATGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(135-244)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

99.656

100

0.997
  pilD Vibrio campbellii strain DS40M4

75.694

98.969

0.749
  pilD Acinetobacter nosocomialis M2

54.044

93.471

0.505
  pilD Acinetobacter baumannii D1279779

53.676

93.471

0.502
  pilD Neisseria gonorrhoeae MS11

45.139

98.969

0.447