Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   IMI45_RS13930 Genome accession   NZ_CP063414
Coordinates   2735558..2736622 (-) Length   354 a.a.
NCBI ID   WP_013400390.1    Uniprot ID   -
Organism   Parageobacillus thermoglucosidasius strain 23.6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2730558..2741622
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMI45_RS13885 (IMI45_13695) - 2731367..2732158 (+) 792 WP_125009488.1 YqhG family protein -
  IMI45_RS13890 (IMI45_13700) - 2732216..2732395 (-) 180 WP_003249195.1 YqzE family protein -
  IMI45_RS13895 (IMI45_13705) - 2732416..2732673 (-) 258 WP_013876629.1 hypothetical protein -
  IMI45_RS13900 (IMI45_13710) comGG 2732663..2733052 (-) 390 WP_125009487.1 competence type IV pilus minor pilin ComGG -
  IMI45_RS13905 (IMI45_13715) comGF 2733069..2733494 (-) 426 WP_125009486.1 competence type IV pilus minor pilin ComGF -
  IMI45_RS13910 (IMI45_13720) comGE 2733491..2733814 (-) 324 WP_125009485.1 competence type IV pilus minor pilin ComGE -
  IMI45_RS13915 (IMI45_13725) comGD 2733798..2734241 (-) 444 WP_003249186.1 competence type IV pilus minor pilin ComGD -
  IMI45_RS13920 (IMI45_13730) comGC 2734225..2734521 (-) 297 WP_125009484.1 competence type IV pilus major pilin ComGC Machinery gene
  IMI45_RS13925 (IMI45_13735) comGB 2734534..2735565 (-) 1032 WP_125009483.1 competence type IV pilus assembly protein ComGB -
  IMI45_RS13930 (IMI45_13740) comGA 2735558..2736622 (-) 1065 WP_013400390.1 competence type IV pilus ATPase ComGA Machinery gene
  IMI45_RS13935 (IMI45_13745) - 2736825..2737523 (+) 699 WP_125009481.1 metalloregulator ArsR/SmtB family transcription factor -
  IMI45_RS13940 (IMI45_13750) - 2737724..2737966 (+) 243 WP_003249178.1 DUF2626 family protein -
  IMI45_RS13945 (IMI45_13755) - 2738017..2739150 (-) 1134 WP_013400389.1 class I SAM-dependent methyltransferase -
  IMI45_RS13950 (IMI45_13760) - 2739484..2740116 (-) 633 WP_003249173.1 MBL fold metallo-hydrolase -
  IMI45_RS13955 (IMI45_13765) - 2740453..2740629 (+) 177 WP_013400388.1 DUF2759 domain-containing protein -
  IMI45_RS13960 (IMI45_13770) - 2740662..2740973 (-) 312 WP_256832813.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39886.36 Da        Isoelectric Point: 9.1564

>NTDB_id=495478 IMI45_RS13930 WP_013400390.1 2735558..2736622(-) (comGA) [Parageobacillus thermoglucosidasius strain 23.6]
MNEIEYLADRLIKEASVLRVSDIHIVPRKDDAAVRFRLDGLLMEKEVLTKEICDRLITHFKFLAGMDIGERRRPQSGAME
TKQQGEIIHLRLSTLPTSYDESLVIRLLPQNLFLPLSQLSLFANAAKTLLSLFQQPQGLIIFTGPAGSGKTTTLYTLLRV
CQSKWNRNVITLEDPVEKRIDNILQVQINERAGITYANGLKAALRHDPNIIMIGEIRDAETAKIAVRSSMTGHLIASTMH
TKNAVGAIYRLREFGIPLHDIEQTLLAVTAQRLVDLVCPFCGEHCSPFCRKHRHIRRAAVHELLYGDALSDAIQSVQNGR
KTYRYYTLQNVIRKGVALGFLPPRLLCAKEGENE

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=495478 IMI45_RS13930 WP_013400390.1 2735558..2736622(-) (comGA) [Parageobacillus thermoglucosidasius strain 23.6]
ATGAATGAAATCGAATATCTTGCCGATCGTCTCATCAAAGAAGCAAGTGTGCTTCGTGTGTCTGACATTCACATCGTTCC
GCGCAAAGACGATGCAGCAGTGCGTTTCCGGCTTGATGGATTGCTTATGGAAAAAGAAGTGCTCACAAAAGAAATATGCG
ATCGCCTGATAACCCATTTTAAGTTTTTAGCCGGAATGGACATTGGCGAACGCCGCCGGCCGCAAAGCGGGGCGATGGAA
ACAAAGCAGCAAGGGGAAATTATTCACTTGCGCCTTTCCACACTGCCGACATCTTACGATGAAAGCCTCGTCATTCGGCT
TCTTCCCCAAAATTTGTTTCTTCCTCTTTCACAGCTTTCTTTATTTGCCAATGCCGCAAAAACATTGCTTTCCCTTTTTC
AGCAACCTCAAGGGTTAATTATTTTCACAGGACCAGCCGGGTCAGGAAAGACGACGACATTGTACACGTTGCTGCGCGTC
TGCCAGTCCAAATGGAACCGCAATGTCATCACATTAGAGGATCCTGTTGAAAAGCGAATCGACAATATATTGCAAGTGCA
AATCAATGAGAGAGCAGGCATCACATATGCAAACGGCTTAAAAGCAGCTTTGCGGCACGATCCAAACATTATTATGATCG
GCGAAATCCGGGATGCCGAAACGGCAAAAATCGCGGTACGTTCGTCGATGACAGGACATTTGATTGCGTCGACAATGCAT
ACGAAAAACGCGGTGGGAGCGATTTACCGTTTGCGTGAATTTGGGATTCCGCTTCATGATATTGAGCAAACGCTGCTTGC
CGTGACAGCGCAGCGGCTTGTCGACTTAGTATGCCCGTTTTGCGGCGAACATTGCTCGCCATTTTGCCGCAAACATCGCC
ACATTCGCCGCGCCGCCGTTCATGAGCTGTTGTATGGAGATGCGCTGTCTGACGCAATTCAATCCGTGCAAAACGGAAGA
AAAACGTATCGTTACTATACGCTGCAAAACGTGATTCGAAAAGGGGTTGCTCTCGGATTTTTGCCGCCACGCCTTCTTTG
CGCCAAGGAGGGAGAAAATGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.848

96.61

0.54
  pilB Haemophilus influenzae 86-028NP

41.25

90.395

0.373
  pilB Glaesserella parasuis strain SC1401

42.105

85.876

0.362