Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   IPV48_RS25220 Genome accession   NZ_CP063386
Coordinates   5386916..5387788 (+) Length   290 a.a.
NCBI ID   WP_003125204.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain ST1076_d118limb1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5381916..5392788
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IPV48_RS25195 (IPV48_25190) nadC 5382257..5383105 (+) 849 WP_003094645.1 carboxylating nicotinate-nucleotide diphosphorylase -
  IPV48_RS25205 (IPV48_25200) pilA 5383304..5383756 (-) 453 WP_031636800.1 pilin Machinery gene
  IPV48_RS25210 (IPV48_25205) pilB 5383988..5385688 (+) 1701 WP_023094327.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IPV48_RS25215 (IPV48_25210) pilC 5385692..5386912 (+) 1221 WP_009315898.1 type 4a pilus biogenesis protein PilC Machinery gene
  IPV48_RS25220 (IPV48_25215) pilD 5386916..5387788 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  IPV48_RS25225 (IPV48_25220) coaE 5387785..5388396 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  IPV48_RS25230 (IPV48_25225) yacG 5388393..5388593 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  IPV48_RS25235 (IPV48_25230) - 5388630..5388839 (-) 210 WP_003094660.1 hypothetical protein -
  IPV48_RS25240 (IPV48_25235) - 5388945..5389634 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  IPV48_RS25245 (IPV48_25240) - 5389631..5390101 (-) 471 WP_003094664.1 hypothetical protein -
  IPV48_RS25250 (IPV48_25245) - 5390098..5390523 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  IPV48_RS25255 (IPV48_25250) - 5390656..5391285 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  IPV48_RS25260 (IPV48_25255) - 5391282..5391731 (+) 450 WP_023100233.1 MOSC domain-containing protein -
  IPV48_RS25265 (IPV48_25260) - 5391757..5391927 (+) 171 WP_003094672.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 31839.89 Da        Isoelectric Point: 7.7068

>NTDB_id=495061 IPV48_RS25220 WP_003125204.1 5386916..5387788(+) (pilD) [Pseudomonas aeruginosa strain ST1076_d118limb1]
MPLLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=495061 IPV48_RS25220 WP_003125204.1 5386916..5387788(+) (pilD) [Pseudomonas aeruginosa strain ST1076_d118limb1]
ATGCCCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTTGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAAGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)

Type IV leader peptidase family (Peptidase_A24)

(137-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.714

96.552

0.538
  pilD Vibrio campbellii strain DS40M4

53.004

97.586

0.517
  pilD Acinetobacter nosocomialis M2

50.53

97.586

0.493
  pilD Acinetobacter baumannii D1279779

50.53

97.586

0.493
  pilD Neisseria gonorrhoeae MS11

49.281

95.862

0.472