Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   AWH56_RS26305 Genome accession   NZ_CP063356
Coordinates   5214004..5215047 (-) Length   347 a.a.
NCBI ID   WP_071317055.1    Uniprot ID   A0A1S2M228
Organism   Anaerobacillus isosaccharinicus strain NB2006     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5209004..5220047
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWH56_RS26275 (AWH56_026275) comGD 5209150..5209605 (-) 456 WP_071317061.1 competence type IV pilus minor pilin ComGD -
  AWH56_RS26280 (AWH56_026280) comGC 5209592..5209915 (-) 324 WP_071317060.1 competence type IV pilus major pilin ComGC -
  AWH56_RS26285 (AWH56_026285) - 5210223..5210591 (+) 369 WP_071317059.1 DUF2500 domain-containing protein -
  AWH56_RS26290 (AWH56_026290) - 5210665..5212260 (-) 1596 WP_182080681.1 putative bifunctional diguanylate cyclase/phosphodiesterase -
  AWH56_RS26295 (AWH56_026295) - 5212558..5212884 (-) 327 WP_071317057.1 DUF2325 domain-containing protein -
  AWH56_RS26300 (AWH56_026300) comGB 5212970..5214004 (-) 1035 WP_071317056.1 competence type IV pilus assembly protein ComGB -
  AWH56_RS26305 (AWH56_026305) comGA 5214004..5215047 (-) 1044 WP_071317055.1 competence type IV pilus ATPase ComGA Machinery gene
  AWH56_RS27170 - 5215034..5215162 (-) 129 WP_274598792.1 hypothetical protein -
  AWH56_RS26315 (AWH56_026315) - 5215489..5216196 (+) 708 WP_071317054.1 helix-turn-helix transcriptional regulator -
  AWH56_RS26320 (AWH56_026320) - 5216316..5216561 (+) 246 WP_071317053.1 DUF2626 domain-containing protein -
  AWH56_RS26325 (AWH56_026325) - 5216676..5217809 (-) 1134 WP_071317052.1 class I SAM-dependent methyltransferase -
  AWH56_RS26330 (AWH56_026330) - 5218020..5218235 (-) 216 WP_071317051.1 cbb3-type cytochrome oxidase assembly protein -
  AWH56_RS26335 (AWH56_026335) - 5218290..5218928 (-) 639 WP_071317050.1 MBL fold metallo-hydrolase -
  AWH56_RS26340 (AWH56_026340) - 5219090..5219566 (+) 477 WP_071317049.1 hypothetical protein -
  AWH56_RS26345 (AWH56_026345) - 5219711..5219884 (+) 174 WP_071317048.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39138.61 Da        Isoelectric Point: 9.8223

>NTDB_id=494604 AWH56_RS26305 WP_071317055.1 5214004..5215047(-) (comGA) [Anaerobacillus isosaccharinicus strain NB2006]
MSIIEQKSDGIISRAVREKASDIHIVPANSSSLIQLRIGHQLVTLEKIKISDAQKLISHYKFRSKMDIGERRRPQNGSLN
MVVKDEQINLRISTLPTTPHESLSIRILPQNEILTIDQLSLFKKHCVQLRTLMKKAYGLLLISGPTSSGKTTTIYSLLFN
EFSRNRRIITIEDPIEKKTDSFIQVEINEKAGLTYAEALKAALRHDPDIIMIGEIRDAQTARIAIRAAMTGHLVISTIHA
NHSEGCISRLREFGCAQLDIKETLIGLVSQRLVNLACPYCGIKCSGYCGLYSTNRRLAVYEILEGNSLQDILANKLKKAK
YEKLPQLVNKAIALGYVKETEYERWLR

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=494604 AWH56_RS26305 WP_071317055.1 5214004..5215047(-) (comGA) [Anaerobacillus isosaccharinicus strain NB2006]
TTGTCAATTATTGAACAAAAAAGTGATGGTATTATTTCACGTGCTGTTCGAGAAAAAGCATCAGATATTCATATCGTTCC
TGCCAATTCCAGTTCTCTAATCCAGCTCAGAATTGGTCATCAATTAGTCACATTGGAAAAAATCAAAATTTCTGATGCCC
AAAAACTCATCTCTCATTATAAGTTTCGCTCGAAAATGGATATTGGCGAGCGAAGAAGACCACAAAATGGATCATTGAAC
ATGGTCGTTAAAGATGAGCAAATTAACTTACGCATTTCTACCTTGCCAACAACCCCTCATGAAAGTCTATCTATTCGAAT
TTTACCTCAAAATGAAATTTTAACCATTGATCAACTCTCACTTTTTAAAAAACATTGCGTGCAACTAAGAACTTTAATGA
AAAAAGCATATGGACTATTATTGATTTCCGGTCCAACGAGTTCGGGAAAGACAACAACTATTTATTCGTTGTTATTTAAT
GAATTTAGTAGGAATCGAAGAATTATTACAATCGAAGATCCTATTGAAAAAAAGACGGATTCCTTTATTCAAGTCGAAAT
AAACGAAAAGGCAGGACTAACATATGCTGAGGCGTTAAAGGCAGCGTTAAGACACGACCCCGATATTATCATGATCGGTG
AGATCCGAGATGCACAGACAGCACGAATTGCCATACGCGCTGCAATGACAGGACATCTAGTTATTAGTACAATCCATGCT
AATCATTCAGAAGGATGTATTTCTAGGCTTAGAGAATTTGGGTGTGCACAGCTTGATATTAAAGAAACACTTATTGGACT
CGTTTCACAAAGACTTGTTAATCTAGCCTGCCCATATTGTGGTATTAAGTGTTCAGGTTATTGTGGATTATACAGTACAA
ATCGAAGGTTAGCTGTATACGAAATTTTGGAAGGCAATTCATTACAAGATATTTTAGCGAATAAGTTGAAAAAAGCTAAG
TATGAAAAGTTACCACAATTAGTTAACAAGGCTATTGCTCTAGGATATGTTAAAGAAACGGAATATGAGAGGTGGCTCCG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1S2M228

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

48.433

100

0.49
  pilB Haemophilus influenzae 86-028NP

36.657

98.271

0.36