Detailed information NTG0494096

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: IHJ54_RS14185
• Genome accession: NZ_CP063241
• Coordinates: 3205095..3205940 (-)
• Length: 281 a.a.
• NCBI ID: WP_040043677.1
• Uniprot ID: -
• Organism: Pectobacterium brasiliense strain ZLMLSHJ5
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3200095..3210940

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IHJ54_RS14160 (IHJ54_14145)	-	3200296..3201066 (-)	771	WP_040043674.1	ABC transporter ATP-binding protein	-
IHJ54_RS14165 (IHJ54_14150)	-	3201068..3202105 (-)	1038	WP_040043675.1	FecCD family ABC transporter permease	-
IHJ54_RS14170 (IHJ54_14155)	-	3202102..3203166 (-)	1065	WP_040043676.1	ABC transporter substrate-binding protein	-
IHJ54_RS14175 (IHJ54_14160)	-	3203477..3204502 (+)	1026	WP_039520406.1	LLM class flavin-dependent oxidoreductase	-
IHJ54_RS14180 (IHJ54_14165)	ypdK	3204860..3204934 (+)	75	WP_099606229.1	membrane protein YpdK	-
IHJ54_RS14185 (IHJ54_14170)	pilD	3205095..3205940 (-)	846	WP_040043677.1	prepilin peptidase	Machinery gene
IHJ54_RS14190 (IHJ54_14175)	-	3206049..3206795 (-)	747	WP_040043678.1	type II secretion system protein N	-
IHJ54_RS14195 (IHJ54_14180)	gspM	3206785..3207279 (-)	495	WP_040043679.1	type II secretion system protein GspM	-
IHJ54_RS14200 (IHJ54_14185)	gspL	3207276..3208553 (-)	1278	WP_040043680.1	type II secretion system protein GspL	-
IHJ54_RS14205 (IHJ54_14190)	gspK	3208550..3209536 (-)	987	WP_040043681.1	type II secretion system minor pseudopilin GspK	-
IHJ54_RS14210 (IHJ54_14195)	gspJ	3209533..3210183 (-)	651	WP_072035873.1	type II secretion system minor pseudopilin GspJ	-
IHJ54_RS14215 (IHJ54_14200)	gspI	3210183..3210548 (-)	366	WP_039273738.1	type II secretion system minor pseudopilin GspI	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 31439.39 Da
• Isoelectric Point: 5.7656

>NTDB_id=494096 IHJ54_RS14185 WP_040043677.1 3205095..3205940(-) (pilD) [Pectobacterium brasiliense strain ZLMLSHJ5]
MDDLREFAQLFPAWWFGSLGVLGLIVGSFLNVVIYRLPIMLERRWRQDIELETGMVDSDPDTRYNLWWPPSSCPHCQQAI
AVKDNIPLFSWLWLRGRSRCCHQSVSVQYPLVEVITMLAFLTAGLLWLPGMALWGALILLSFLLVLTVIDIKTLLLPDVL
TLSLLWIGLLFNLSETFVPLNDAVVGAMAGYLSLWLLYWVFKYATGKEALGYGDFKLLAALGAWLGWQALPNLVLVAALS
GLVVTLVWRGLRKEDTAKPLAFGPWLAMGGVFGVIMNGLNL

Nucleotide

• Length: 846 bp

>NTDB_id=494096 IHJ54_RS14185 WP_040043677.1 3205095..3205940(-) (pilD) [Pectobacterium brasiliense strain ZLMLSHJ5]
GTGGACGATTTAAGGGAATTTGCACAGCTGTTTCCGGCGTGGTGGTTTGGATCGCTGGGCGTGCTGGGATTGATTGTTGG
CAGTTTTCTAAACGTGGTGATTTACCGCTTGCCGATTATGCTGGAGCGTCGCTGGCGGCAGGACATCGAGCTTGAAACGG
GCATGGTCGATTCCGATCCTGATACGCGCTACAACCTGTGGTGGCCGCCTTCGTCGTGCCCGCACTGCCAGCAGGCTATT
GCGGTAAAAGATAACATCCCGCTATTCAGCTGGTTGTGGTTGCGCGGCCGCTCCCGCTGCTGTCATCAATCGGTGTCTGT
GCAATATCCGTTAGTGGAAGTCATCACCATGTTGGCTTTTCTGACGGCAGGTTTACTCTGGTTGCCCGGGATGGCGCTGT
GGGGGGCGTTGATTCTGCTGTCATTCCTGTTGGTGCTGACCGTTATTGATATAAAAACGCTGCTGCTGCCGGATGTGCTT
ACGCTGTCGCTTCTGTGGATAGGGCTGCTGTTTAATCTGTCGGAGACATTTGTCCCATTGAATGATGCCGTAGTCGGCGC
GATGGCCGGTTATTTATCTCTGTGGCTGCTTTATTGGGTATTCAAATACGCAACGGGAAAAGAGGCGCTGGGGTACGGTG
ATTTTAAGTTGCTGGCTGCGCTGGGTGCCTGGCTGGGCTGGCAAGCATTGCCGAACCTGGTTCTGGTGGCGGCGCTGAGC
GGGTTGGTCGTGACTCTCGTCTGGCGCGGATTGCGTAAAGAGGATACGGCTAAACCGCTGGCCTTTGGCCCCTGGCTGGC
AATGGGCGGGGTATTTGGCGTGATAATGAACGGATTGAATCTGTAG

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-123)

• Type IV leader peptidase family (Peptidase_A24) (137-245)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.185	96.441	0.484
pilD	Neisseria gonorrhoeae MS11	47.126	92.883	0.438
pilD	Vibrio campbellii strain DS40M4	44.981	95.73	0.431
pilD	Acinetobacter baumannii D1279779	41.758	97.153	0.406
pilD	Acinetobacter nosocomialis M2	41.392	97.153	0.402