Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   IMY50_RS21135 Genome accession   NZ_CP063158
Coordinates   4156983..4158026 (-) Length   347 a.a.
NCBI ID   WP_001013187.1    Uniprot ID   -
Organism   Bacillus cereus strain CF4-51     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4151983..4163026
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMY50_RS21090 (IMY50_21085) - 4152416..4152616 (-) 201 WP_000106083.1 YqzE family protein -
  IMY50_RS21095 (IMY50_21090) - 4152655..4153152 (-) 498 WP_000836666.1 shikimate kinase -
  IMY50_RS21100 (IMY50_21095) - 4153268..4153918 (-) 651 WP_000183866.1 2OG-Fe(II) oxygenase -
  IMY50_RS21105 (IMY50_21100) comGG 4154093..4154464 (-) 372 WP_023523011.1 competence type IV pilus minor pilin ComGG -
  IMY50_RS21110 (IMY50_21105) comGF 4154461..4154931 (-) 471 WP_000923097.1 competence type IV pilus minor pilin ComGF -
  IMY50_RS21115 (IMY50_21110) - 4154901..4155203 (-) 303 WP_000829462.1 hypothetical protein -
  IMY50_RS21120 (IMY50_21115) comGD 4155196..4155651 (-) 456 WP_002094140.1 competence type IV pilus minor pilin ComGD -
  IMY50_RS21125 (IMY50_21120) comGC 4155648..4155947 (-) 300 WP_001178687.1 competence type IV pilus major pilin ComGC -
  IMY50_RS21130 (IMY50_21125) comGB 4155959..4156990 (-) 1032 WP_081367148.1 competence type IV pilus assembly protein ComGB -
  IMY50_RS21135 (IMY50_21130) comGA 4156983..4158026 (-) 1044 WP_001013187.1 competence type IV pilus ATPase ComGA Machinery gene
  IMY50_RS21140 (IMY50_21135) - 4158232..4158927 (+) 696 WP_000434107.1 helix-turn-helix domain-containing protein -
  IMY50_RS21145 (IMY50_21140) - 4159054..4159296 (+) 243 WP_000375619.1 DUF2626 domain-containing protein -
  IMY50_RS21150 (IMY50_21145) - 4159404..4160798 (+) 1395 WP_001094344.1 L-cystine transporter -
  IMY50_RS21155 (IMY50_21150) - 4160878..4161270 (-) 393 WP_071713577.1 hypothetical protein -
  IMY50_RS21160 (IMY50_21155) - 4161358..4161573 (-) 216 WP_001008326.1 DUF3912 family protein -
  IMY50_RS21165 (IMY50_21160) - 4161827..4162138 (+) 312 WP_001093240.1 hypothetical protein -
  IMY50_RS21170 (IMY50_21165) - 4162173..4162661 (-) 489 WP_000764263.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39164.52 Da        Isoelectric Point: 8.5432

>NTDB_id=493645 IMY50_RS21135 WP_001013187.1 4156983..4158026(-) (comGA) [Bacillus cereus strain CF4-51]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRVGKDLMTRHCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEADGEC
VTYKHETLESSIRKGYALGFLEDDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=493645 IMY50_RS21135 WP_001013187.1 4156983..4158026(-) (comGA) [Bacillus cereus strain CF4-51]
ATGAATGGGATTGAAATTTTTGCGAATACAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTGTAGGAAAAGATTTAATGACGAGACATTGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTTAAATTTTTAGCATCTATGGACATAGGAGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTGCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTCGTGTTTACTGGACCGACTGGTTCTGGGAAGACAACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTCATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAAGCAAGCATTTATGAGCTGCTATATGGATATGAGTTAAAACAAGCGCTTAAAGAAGCAGATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATAAGAAAAGGATATGCTTTAGGTTTTTTAGAAGATGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.196

100

0.562
  pilB Haemophilus influenzae 86-028NP

39.441

92.795

0.366