Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   INP89_RS08460 Genome accession   NZ_CP063127
Coordinates   1703254..1703793 (-) Length   179 a.a.
NCBI ID   WP_012054975.1    Uniprot ID   -
Organism   Haemophilus influenzae strain M1C112_1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1698254..1708793
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP89_RS08430 (INP89_08425) fdx 1698657..1698998 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  INP89_RS08435 (INP89_08430) iscX 1698998..1699192 (+) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  INP89_RS08440 (INP89_08435) - 1699243..1699857 (-) 615 WP_005691752.1 YfgM family protein -
  INP89_RS08445 (INP89_08440) hisS 1699875..1701146 (-) 1272 WP_012054977.1 histidine--tRNA ligase -
  INP89_RS08450 (INP89_08445) ispG 1701156..1702262 (-) 1107 WP_005649145.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  INP89_RS08455 (INP89_08450) - 1702271..1703182 (-) 912 WP_012054976.1 helix-turn-helix domain-containing protein -
  INP89_RS08460 (INP89_08455) pilF/pilF2 1703254..1703793 (-) 540 WP_012054975.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  INP89_RS08465 (INP89_08460) - 1703894..1705045 (-) 1152 WP_005662659.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  INP89_RS08470 (INP89_08465) - 1705292..1706170 (+) 879 WP_012054974.1 D-alanyl-D-alanine carboxypeptidase family protein -
  INP89_RS08475 (INP89_08470) - 1706536..1707417 (+) 882 WP_005662662.1 metal ABC transporter substrate-binding protein -
  INP89_RS08480 (INP89_08475) - 1707417..1708337 (+) 921 WP_005662663.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20554.15 Da        Isoelectric Point: 8.2571

>NTDB_id=493244 INP89_RS08460 WP_012054975.1 1703254..1703793(-) (pilF/pilF2) [Haemophilus influenzae strain M1C112_1]
MKTISKQLSAVIFPFIFSACVSQSASSLNHQAAAKARVELALSYLQQNNPQLAKINLDNALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=493244 INP89_RS08460 WP_012054975.1 1703254..1703793(-) (pilF/pilF2) [Haemophilus influenzae strain M1C112_1]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTTATTTTTTCTGCTTGCGTTTCACAATCTGCATCTAG
CTTAAATCATCAAGCTGCCGCTAAAGCGCGCGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAACTTAGATAACGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCTCAGCAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATTGTGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

98.883

100

0.989
  pilF Glaesserella parasuis strain SC1401

44.134

100

0.441