Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   INP90_RS09815 Genome accession   NZ_CP063124
Coordinates   2017869..2018411 (-) Length   180 a.a.
NCBI ID   WP_197559652.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae strain M1C113_1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2012869..2023411
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP90_RS09790 (INP90_09790) - 2013206..2013649 (-) 444 WP_197559648.1 methyltransferase family protein -
  INP90_RS09795 (INP90_09795) - 2013700..2014314 (-) 615 WP_197559649.1 YfgM family protein -
  INP90_RS09800 (INP90_09800) hisS 2014333..2015604 (-) 1272 WP_197559650.1 histidine--tRNA ligase -
  INP90_RS09805 (INP90_09805) ispG 2015614..2016720 (-) 1107 WP_197555853.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  INP90_RS09810 (INP90_09810) - 2016739..2017794 (-) 1056 WP_197559651.1 RodZ domain-containing protein -
  INP90_RS09815 (INP90_09815) pilF/pilF2 2017869..2018411 (-) 543 WP_197559652.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  INP90_RS09820 (INP90_09820) - 2018481..2019629 (-) 1149 WP_197559653.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  INP90_RS09825 (INP90_09825) - 2019657..2019842 (+) 186 WP_232090017.1 hypothetical protein -
  INP90_RS09830 (INP90_09830) - 2019933..2020808 (+) 876 WP_054419668.1 D-alanyl-D-alanine carboxypeptidase family protein -
  INP90_RS09835 (INP90_09835) - 2020963..2021844 (+) 882 WP_049357006.1 metal ABC transporter substrate-binding protein -
  INP90_RS09840 (INP90_09840) - 2021844..2022740 (+) 897 WP_054419670.1 manganese/iron ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20356.95 Da        Isoelectric Point: 6.4768

>NTDB_id=493215 INP90_RS09815 WP_197559652.1 2017869..2018411(-) (pilF/pilF2) [Haemophilus parainfluenzae strain M1C113_1]
MKLIPINILSAVIFPFVFSACVSQSSVDFNKQQAAKARVELALGYLQQNDFVQAKLNLDKAFEHDDRYYLVHSALAHFYQ
LQGDTEKAKQAYLQAIKLDDKQGDVYNNFGAFLCGQGEFEQAYSQFNAALAAPNYYHQADTYENMALCAFAGKQTDVYQQ
ALDKLRQVDSSRAEKLRSLK

Nucleotide


Download         Length: 543 bp        

>NTDB_id=493215 INP90_RS09815 WP_197559652.1 2017869..2018411(-) (pilF/pilF2) [Haemophilus parainfluenzae strain M1C113_1]
ATGAAATTAATCCCAATTAACATTCTAAGTGCGGTCATTTTTCCTTTTGTTTTTTCTGCTTGTGTTTCTCAGTCTTCTGT
TGATTTTAATAAACAACAAGCCGCAAAAGCCCGAGTTGAATTGGCATTAGGCTATCTTCAACAAAATGATTTCGTTCAAG
CAAAACTGAATTTAGATAAAGCCTTTGAACATGATGACCGCTATTATCTTGTGCACTCGGCACTTGCGCATTTTTATCAA
TTACAAGGCGATACGGAAAAAGCGAAGCAAGCTTATTTACAGGCGATTAAGCTAGACGATAAGCAAGGCGATGTGTATAA
CAACTTCGGTGCATTTTTATGTGGACAAGGTGAGTTTGAACAAGCTTATTCACAATTTAATGCGGCACTCGCTGCGCCGA
ATTATTACCATCAAGCCGATACTTACGAAAACATGGCGCTTTGTGCTTTCGCCGGAAAACAAACCGATGTTTATCAACAG
GCATTGGATAAATTGCGTCAAGTTGATTCTTCTAGAGCGGAAAAGCTCCGTTCACTCAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(74-101)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

65.698

95.556

0.628
  pilF Glaesserella parasuis strain SC1401

49.153

98.333

0.483