Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   INP91_RS08375 Genome accession   NZ_CP063123
Coordinates   1738386..1738928 (-) Length   180 a.a.
NCBI ID   WP_197545799.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae strain M1C120_2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1733386..1743928
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP91_RS08350 (INP91_08345) - 1733746..1734189 (-) 444 WP_197545795.1 methyltransferase family protein -
  INP91_RS08355 (INP91_08350) - 1734241..1734855 (-) 615 WP_197545796.1 YfgM family protein -
  INP91_RS08360 (INP91_08355) hisS 1734874..1736145 (-) 1272 WP_197545797.1 histidine--tRNA ligase -
  INP91_RS08365 (INP91_08360) ispG 1736155..1737261 (-) 1107 WP_193451359.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  INP91_RS08370 (INP91_08365) - 1737280..1738311 (-) 1032 WP_197545798.1 RodZ domain-containing protein -
  INP91_RS08375 (INP91_08370) pilF/pilF2 1738386..1738928 (-) 543 WP_197545799.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  INP91_RS08380 (INP91_08375) - 1738998..1740146 (-) 1149 WP_197545800.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  INP91_RS08385 (INP91_08380) - 1740450..1741325 (+) 876 WP_197545801.1 D-alanyl-D-alanine carboxypeptidase family protein -
  INP91_RS08390 (INP91_08385) - 1741479..1742360 (+) 882 WP_197545802.1 metal ABC transporter substrate-binding protein -
  INP91_RS08395 (INP91_08390) - 1742360..1743256 (+) 897 WP_197545803.1 manganese/iron ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20381.06 Da        Isoelectric Point: 6.8526

>NTDB_id=493177 INP91_RS08375 WP_197545799.1 1738386..1738928(-) (pilF/pilF2) [Haemophilus parainfluenzae strain M1C120_2]
MKLIPINILSAVIFPFVFSACVSQSSVDFNKQQAAKARVELALGYLQQNDFVQAKLNLDKALEHDERYYLVHSAFAHFYQ
LQGDTEKAKQAYLKAIKLDDKQGDVYNNFGAFLCGQGEFEQAYSQFNAALAAPNYYHQADTYENMALCAFAGKQTDVYQQ
ALDKLRQVDPSRAEKLRSLK

Nucleotide


Download         Length: 543 bp        

>NTDB_id=493177 INP91_RS08375 WP_197545799.1 1738386..1738928(-) (pilF/pilF2) [Haemophilus parainfluenzae strain M1C120_2]
ATGAAATTAATCCCAATTAACATTCTAAGTGCGGTCATTTTTCCTTTTGTTTTTTCTGCTTGTGTCTCTCAGTCTTCCGT
TGATTTTAATAAACAACAAGCCGCAAAAGCCCGCGTTGAATTGGCATTAGGCTATCTTCAACAAAATGATTTTGTTCAAG
CAAAACTTAATTTAGATAAAGCCCTTGAACATGATGAACGCTATTACCTTGTGCATTCTGCATTTGCACATTTTTATCAA
TTACAAGGTGATACAGAAAAAGCGAAGCAAGCTTATTTAAAGGCGATTAAGTTAGACGATAAGCAAGGCGACGTGTATAA
CAATTTTGGTGCATTTTTATGTGGACAAGGTGAGTTTGAACAAGCTTATTCACAATTTAATGCAGCTCTTGCTGCGCCAA
ATTATTATCATCAAGCTGATACTTACGAAAATATGGCGCTTTGTGCTTTTGCTGGAAAACAAACTGATGTTTATCAACAG
GCATTGGATAAATTGCGTCAAGTTGATCCTTCTAGAGCGGAAAAGCTCCGTTCACTCAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(75-101)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

65.698

95.556

0.628
  pilF Glaesserella parasuis strain SC1401

48.588

98.333

0.478