Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   INP92_RS09260 Genome accession   NZ_CP063122
Coordinates   1932154..1932696 (-) Length   180 a.a.
NCBI ID   WP_049364987.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae strain M1C125_4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1927154..1937696
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP92_RS09235 (INP92_09230) - 1927517..1927960 (-) 444 WP_049364992.1 methyltransferase family protein -
  INP92_RS09240 (INP92_09235) - 1928012..1928626 (-) 615 WP_049364991.1 YfgM family protein -
  INP92_RS09245 (INP92_09240) hisS 1928645..1929916 (-) 1272 WP_049364989.1 histidine--tRNA ligase -
  INP92_RS09250 (INP92_09245) ispG 1929926..1931032 (-) 1107 WP_049363739.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  INP92_RS09255 (INP92_09250) - 1931051..1932079 (-) 1029 WP_049364988.1 RodZ domain-containing protein -
  INP92_RS09260 (INP92_09255) pilF/pilF2 1932154..1932696 (-) 543 WP_049364987.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  INP92_RS09265 (INP92_09260) - 1932766..1933914 (-) 1149 WP_049363743.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  INP92_RS09270 (INP92_09265) - 1934215..1935090 (+) 876 WP_049364986.1 D-alanyl-D-alanine carboxypeptidase family protein -
  INP92_RS09275 (INP92_09270) - 1935245..1936126 (+) 882 WP_049364985.1 metal ABC transporter substrate-binding protein -
  INP92_RS09280 (INP92_09275) - 1936126..1937022 (+) 897 WP_049364984.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20347.00 Da        Isoelectric Point: 6.4774

>NTDB_id=493153 INP92_RS09260 WP_049364987.1 1932154..1932696(-) (pilF/pilF2) [Haemophilus parainfluenzae strain M1C125_4]
MKLIPINILSAVIFPFVFSACVSQSSVDFNKQQAAKARVELALGYLQQNDFVQAKLNLDKALEHDERYYLVHSALAHFYQ
LQGDTEKAKQAYLQAIKLDDKQGDVYNNFGAFLCGQGEFEQAYSQFNAALAAPNYYHQADTYENMALCAFAGKQTDVYQQ
ALDKLRQVDPSRAEKLRSLK

Nucleotide


Download         Length: 543 bp        

>NTDB_id=493153 INP92_RS09260 WP_049364987.1 1932154..1932696(-) (pilF/pilF2) [Haemophilus parainfluenzae strain M1C125_4]
ATGAAATTAATCCCAATTAACATTCTAAGTGCGGTCATTTTTCCTTTTGTTTTTTCTGCTTGTGTCTCTCAGTCTTCCGT
TGACTTTAATAAACAACAAGCAGCAAAAGCACGAGTTGAACTGGCATTAGGCTATCTTCAACAAAATGATTTTGTTCAAG
CAAAACTTAATTTAGATAAAGCCCTTGAACATGATGAACGATATTATCTTGTGCACTCAGCACTTGCGCATTTTTATCAA
TTACAAGGCGATACAGAAAAAGCGAAGCAAGCTTATTTACAGGCGATTAAGTTAGATGATAAGCAAGGCGATGTGTATAA
CAACTTCGGTGCTTTTTTATGTGGGCAAGGTGAGTTTGAACAAGCTTATTCACAATTTAATGCGGCACTCGCTGCGCCGA
ATTATTACCATCAGGCCGATACTTACGAAAACATGGCGCTTTGTGCTTTCGCCGGAAAACAAACCGATGTTTATCAACAG
GCATTGGATAAATTGCGTCAAGTTGATCCTTCTAGAGCGGAAAAGCTCCGTTCACTCAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(74-101)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

66.279

95.556

0.633
  pilF Glaesserella parasuis strain SC1401

49.718

98.333

0.489