Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   INP93_RS09585 Genome accession   NZ_CP063121
Coordinates   1978317..1978859 (-) Length   180 a.a.
NCBI ID   WP_197544786.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae strain M1C130_2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1973317..1983859
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP93_RS09560 (INP93_09555) - 1973667..1974110 (-) 444 WP_197544781.1 methyltransferase family protein -
  INP93_RS09565 (INP93_09560) - 1974162..1974776 (-) 615 WP_197544782.1 YfgM family protein -
  INP93_RS09570 (INP93_09565) hisS 1974795..1976066 (-) 1272 WP_197544783.1 histidine--tRNA ligase -
  INP93_RS09575 (INP93_09570) ispG 1976076..1977182 (-) 1107 WP_197544784.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  INP93_RS09580 (INP93_09575) - 1977201..1978229 (-) 1029 WP_197544785.1 RodZ domain-containing protein -
  INP93_RS09585 (INP93_09580) pilF/pilF2 1978317..1978859 (-) 543 WP_197544786.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  INP93_RS09590 (INP93_09585) - 1978929..1980077 (-) 1149 WP_049363743.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  INP93_RS09595 (INP93_09590) - 1980379..1981254 (+) 876 WP_049363744.1 D-alanyl-D-alanine carboxypeptidase family protein -
  INP93_RS09600 (INP93_09595) - 1981408..1982289 (+) 882 WP_126471928.1 metal ABC transporter substrate-binding protein -
  INP93_RS09605 (INP93_09600) - 1982289..1983185 (+) 897 WP_070582499.1 manganese/iron ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20396.02 Da        Isoelectric Point: 6.0886

>NTDB_id=493122 INP93_RS09585 WP_197544786.1 1978317..1978859(-) (pilF/pilF2) [Haemophilus parainfluenzae strain M1C130_2]
MKLIPINILSAVIFPFVFSACVSQSSVDFNKQQAAKARVELALGYLQQEDFVQAKLNLDKAFEHDDRYYLVHSALAHFYQ
LQGDTEKAKQAYLQAIKLDDKQGDVYNNFGAFLCGQGEFEQAYSQFNAALAAPNYYHQADTYENMALCAFAGKQTDVYQQ
ALDKLRQVDPSRAEKLRTLK

Nucleotide


Download         Length: 543 bp        

>NTDB_id=493122 INP93_RS09585 WP_197544786.1 1978317..1978859(-) (pilF/pilF2) [Haemophilus parainfluenzae strain M1C130_2]
ATGAAATTAATCCCAATTAACATTCTAAGTGCGGTCATTTTTCCTTTTGTTTTTTCTGCTTGCGTTTCTCAATCTTCAGT
CGATTTTAATAAACAACAAGCAGCCAAAGCGCGCGTTGAGTTGGCATTAGGCTATCTGCAACAAGAGGATTTTGTTCAAG
CAAAACTTAATTTAGATAAAGCCTTTGAACATGATGACCGCTATTATCTTGTGCACTCGGCACTTGCGCATTTTTATCAA
TTACAAGGCGATACGGAAAAAGCGAAGCAAGCTTATTTACAGGCGATTAAGTTAGACGATAAGCAAGGTGATGTGTATAA
CAACTTCGGTGCTTTTTTATGTGGGCAAGGTGAGTTTGAACAAGCTTATTCACAATTTAATGCGGCACTCGCTGCACCGA
ATTACTATCATCAAGCTGATACTTACGAAAATATGGCACTTTGTGCTTTTGCCGGAAAACAAACCGATGTTTATCAACAA
GCGTTGGATAAATTACGCCAAGTTGATCCTTCTAGAGCGGAAAAGCTCCGCACGCTCAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(74-101)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

64.535

95.556

0.617
  pilF Glaesserella parasuis strain SC1401

49.153

98.333

0.483