Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   INP95_RS09575 Genome accession   NZ_CP063117
Coordinates   1993614..1994156 (-) Length   180 a.a.
NCBI ID   WP_049385013.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae strain M1C142_1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1988614..1999156
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP95_RS09550 (INP95_09545) - 1988978..1989421 (-) 444 WP_197560641.1 methyltransferase family protein -
  INP95_RS09555 (INP95_09550) - 1989473..1990087 (-) 615 WP_178411184.1 YfgM family protein -
  INP95_RS09560 (INP95_09555) hisS 1990106..1991377 (-) 1272 WP_178164415.1 histidine--tRNA ligase -
  INP95_RS09565 (INP95_09560) ispG 1991387..1992493 (-) 1107 WP_049363739.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  INP95_RS09570 (INP95_09565) - 1992512..1993540 (-) 1029 WP_049385012.1 RodZ domain-containing protein -
  INP95_RS09575 (INP95_09570) pilF/pilF2 1993614..1994156 (-) 543 WP_049385013.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  INP95_RS09580 (INP95_09575) - 1994226..1995374 (-) 1149 WP_049385014.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  INP95_RS09585 (INP95_09580) - 1995676..1996551 (+) 876 WP_049363744.1 D-alanyl-D-alanine carboxypeptidase family protein -
  INP95_RS09590 (INP95_09585) - 1996705..1997586 (+) 882 WP_070590595.1 metal ABC transporter substrate-binding protein -
  INP95_RS09595 (INP95_09590) - 1997586..1998482 (+) 897 WP_049372887.1 manganese/iron ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20328.96 Da        Isoelectric Point: 6.4774

>NTDB_id=493059 INP95_RS09575 WP_049385013.1 1993614..1994156(-) (pilF/pilF2) [Haemophilus parainfluenzae strain M1C142_1]
MKLIPINILSAVIFPFVFSACVSQSSVDFNKQQAAKARVELALGYLQQNDFVQAKLNLDKALEHDERYYLVHSALAHFYQ
LQGDTEKAKQAYLQAIKLDDKQGDVYNNFGAFLCGQGEFEQAYSQFNAALAAPNYYHQADTYENIALCAFAGKQTDVYQQ
ALDKLRQVDPSRAEKLRSLK

Nucleotide


Download         Length: 543 bp        

>NTDB_id=493059 INP95_RS09575 WP_049385013.1 1993614..1994156(-) (pilF/pilF2) [Haemophilus parainfluenzae strain M1C142_1]
ATGAAATTAATCCCAATTAACATTCTAAGTGCGGTCATTTTTCCTTTTGTTTTTTCGGCTTGTGTCTCTCAGTCTTCCGT
TGACTTTAATAAACAACAAGCCGCAAAAGCTCGCGTTGAACTGGCATTAGGCTATCTTCAACAAAATGATTTTGTTCAAG
CAAAACTTAATTTAGATAAAGCCCTTGAACATGATGAGCGATATTATCTTGTGCACTCAGCACTTGCGCATTTTTATCAA
TTACAAGGCGATACGGAAAAAGCGAAGCAAGCTTATTTACAGGCGATCAAGTTAGATGATAAGCAAGGCGATGTGTATAA
CAACTTCGGTGCATTTTTATGTGGACAAGGTGAGTTTGAACAAGCTTATTCACAATTTAATGCGGCACTCGCTGCGCCGA
ATTATTACCATCAAGCGGATACTTACGAAAACATAGCGCTTTGTGCTTTTGCTGGAAAACAAACCGATGTTTATCAACAG
GCATTGGATAAATTGCGTCAAGTTGATCCTTCTAGAGCGGAAAAGCTCCGTTCACTCAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(74-101)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

66.86

95.556

0.639
  pilF Glaesserella parasuis strain SC1401

50.282

98.333

0.494