Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   INP96_RS09255 Genome accession   NZ_CP063116
Coordinates   1910254..1910796 (-) Length   180 a.a.
NCBI ID   WP_197555855.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae strain M1C146_1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1905254..1915796
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP96_RS09230 (INP96_09225) - 1905615..1906058 (-) 444 WP_197555851.1 methyltransferase family protein -
  INP96_RS09235 (INP96_09230) - 1906109..1906723 (-) 615 WP_049364991.1 YfgM family protein -
  INP96_RS09240 (INP96_09235) hisS 1906742..1908013 (-) 1272 WP_197555852.1 histidine--tRNA ligase -
  INP96_RS09245 (INP96_09240) ispG 1908023..1909129 (-) 1107 WP_197555853.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  INP96_RS09250 (INP96_09245) - 1909148..1910179 (-) 1032 WP_197555854.1 RodZ domain-containing protein -
  INP96_RS09255 (INP96_09250) pilF/pilF2 1910254..1910796 (-) 543 WP_197555855.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  INP96_RS09260 (INP96_09255) - 1910866..1912014 (-) 1149 WP_049363743.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  INP96_RS09265 (INP96_09260) - 1912317..1913192 (+) 876 WP_197555856.1 D-alanyl-D-alanine carboxypeptidase family protein -
  INP96_RS09270 (INP96_09265) - 1913346..1914227 (+) 882 WP_126471928.1 metal ABC transporter substrate-binding protein -
  INP96_RS09275 (INP96_09270) - 1914227..1915123 (+) 897 WP_070582499.1 manganese/iron ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20435.04 Da        Isoelectric Point: 6.0918

>NTDB_id=493026 INP96_RS09255 WP_197555855.1 1910254..1910796(-) (pilF/pilF2) [Haemophilus parainfluenzae strain M1C146_1]
MKLIPINILSAVIFPFVFSACVSQSSVDFNKQQAAKARVELALGYLQQNDFVQAKLNLDKAFEHDERYYLVHSALAHFYQ
LQGDTEKAKQAYLQAIKLDDKQGDVYNNFGAFLCGQGEFEQAYSQFNEALAAPNYYHQADTYENIALCAFAGKQTDVYQQ
ALEKLRQVDPSRAEKLRSLK

Nucleotide


Download         Length: 543 bp        

>NTDB_id=493026 INP96_RS09255 WP_197555855.1 1910254..1910796(-) (pilF/pilF2) [Haemophilus parainfluenzae strain M1C146_1]
ATGAAATTAATCCCAATTAACATTCTAAGTGCGGTCATTTTTCCTTTTGTTTTTTCTGCTTGTGTCTCTCAGTCTTCCGT
TGACTTTAATAAACAACAAGCAGCAAAAGCCCGAGTTGAACTGGCATTAGGCTATCTTCAACAAAATGATTTTGTTCAAG
CAAAACTTAATTTAGATAAAGCCTTTGAACATGATGAACGATATTATCTTGTGCACTCGGCACTTGCGCATTTTTATCAA
TTACAAGGCGATACGGAAAAAGCGAAGCAAGCTTATTTACAGGCGATTAAGTTAGACGATAAGCAAGGTGATGTGTATAA
CAACTTTGGTGCATTTTTATGTGGACAAGGTGAGTTTGAACAAGCTTATTCACAATTTAATGAGGCACTTGCAGCACCGA
ATTATTATCATCAAGCTGATACTTACGAAAACATAGCACTTTGTGCTTTTGCTGGAAAACAAACCGACGTTTATCAACAA
GCATTAGAGAAATTGCGTCAAGTTGATCCTTCTAGAGCGGAAAAGCTCCGTTCACTCAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(74-101)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

66.86

95.556

0.639
  pilF Glaesserella parasuis strain SC1401

49.718

98.333

0.489