Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   INP97_RS09305 Genome accession   NZ_CP063113
Coordinates   1926637..1927179 (-) Length   180 a.a.
NCBI ID   WP_197562653.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae strain M1C147_1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1921637..1932179
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP97_RS09280 (INP97_09260) - 1922000..1922443 (-) 444 WP_197562650.1 methyltransferase family protein -
  INP97_RS09285 (INP97_09265) - 1922495..1923109 (-) 615 WP_178160973.1 YfgM family protein -
  INP97_RS09290 (INP97_09270) hisS 1923128..1924399 (-) 1272 WP_197562651.1 histidine--tRNA ligase -
  INP97_RS09295 (INP97_09275) ispG 1924409..1925515 (-) 1107 WP_049363739.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  INP97_RS09300 (INP97_09280) - 1925534..1926562 (-) 1029 WP_197562652.1 RodZ domain-containing protein -
  INP97_RS09305 (INP97_09285) pilF/pilF2 1926637..1927179 (-) 543 WP_197562653.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  INP97_RS09310 (INP97_09290) - 1927249..1928397 (-) 1149 WP_197562654.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  INP97_RS09315 (INP97_09295) - 1928699..1929574 (+) 876 WP_049364986.1 D-alanyl-D-alanine carboxypeptidase family protein -
  INP97_RS09320 (INP97_09300) - 1929715..1930596 (+) 882 WP_126471928.1 metal ABC transporter substrate-binding protein -
  INP97_RS09325 (INP97_09305) - 1930596..1931492 (+) 897 WP_197562655.1 manganese/iron ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20314.94 Da        Isoelectric Point: 6.4774

>NTDB_id=492995 INP97_RS09305 WP_197562653.1 1926637..1927179(-) (pilF/pilF2) [Haemophilus parainfluenzae strain M1C147_1]
MKLIPINILSAVIFPFVFSACVSQSSVDFNKQQAAKARVELALGYLQQNDFVQAKLNLDKALEHDERYYLVHSALGHFYQ
LQGDTEKAKQAYLQAIKLDDKQGDVYNNFGAFLCGQGEFEQAYSQFNAALAAPNYYHQADTYENIALCAFAGKQTDVYQQ
ALDKLRQVDPSRAEKLRSLK

Nucleotide


Download         Length: 543 bp        

>NTDB_id=492995 INP97_RS09305 WP_197562653.1 1926637..1927179(-) (pilF/pilF2) [Haemophilus parainfluenzae strain M1C147_1]
ATGAAATTAATCCCAATTAACATTCTAAGTGCGGTCATTTTTCCTTTTGTTTTTTCTGCTTGTGTCTCTCAGTCTTCCGT
TGATTTTAATAAACAACAAGCCGCAAAAGCTCGTGTTGAATTAGCATTAGGCTATCTTCAACAAAATGATTTTGTTCAAG
CAAAACTCAATTTAGATAAAGCCCTTGAGCATGATGAACGATATTATCTTGTGCACTCAGCACTTGGGCATTTTTATCAA
TTACAAGGCGATACAGAAAAAGCGAAGCAAGCTTATTTACAGGCGATTAAGTTAGATGATAAGCAAGGCGATGTGTATAA
CAACTTCGGTGCATTTTTATGTGGACAAGGTGAGTTTGAACAAGCTTATTCACAATTTAATGCGGCACTCGCTGCACCGA
ATTATTACCATCAAGCGGATACTTACGAAAACATAGCGCTTTGTGCTTTTGCTGGAAAACAAACCGATGTTTATCAACAG
GCATTGGATAAATTGCGTCAAGTTGATCCTTCTAGAGCGGAAAAGCTCCGTTCACTCAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(74-101)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

66.279

95.556

0.633
  pilF Glaesserella parasuis strain SC1401

49.718

98.333

0.489