Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   INP98_RS09595 Genome accession   NZ_CP063112
Coordinates   1998979..1999521 (-) Length   180 a.a.
NCBI ID   WP_197569998.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae strain M1C149_1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1993979..2004521
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP98_RS09570 (INP98_09545) - 1994340..1994783 (-) 444 WP_197569995.1 methyltransferase family protein -
  INP98_RS09575 (INP98_09550) - 1994835..1995449 (-) 615 WP_049356996.1 YfgM family protein -
  INP98_RS09580 (INP98_09555) hisS 1995468..1996739 (-) 1272 WP_197569996.1 histidine--tRNA ligase -
  INP98_RS09585 (INP98_09560) ispG 1996749..1997855 (-) 1107 WP_005695590.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  INP98_RS09590 (INP98_09565) - 1997874..1998902 (-) 1029 WP_197569997.1 RodZ domain-containing protein -
  INP98_RS09595 (INP98_09570) pilF/pilF2 1998979..1999521 (-) 543 WP_197569998.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  INP98_RS09600 (INP98_09575) - 1999591..2000739 (-) 1149 WP_197569999.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  INP98_RS09605 (INP98_09580) - 2000767..2001036 (+) 270 WP_197570538.1 hypothetical protein -
  INP98_RS09610 (INP98_09585) - 2001042..2001917 (+) 876 WP_049363744.1 D-alanyl-D-alanine carboxypeptidase family protein -
  INP98_RS09615 (INP98_09590) - 2002071..2002952 (+) 882 WP_111387504.1 metal ABC transporter substrate-binding protein -
  INP98_RS09620 (INP98_09595) - 2002952..2003848 (+) 897 WP_070582499.1 manganese/iron ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20398.99 Da        Isoelectric Point: 6.2186

>NTDB_id=492964 INP98_RS09595 WP_197569998.1 1998979..1999521(-) (pilF/pilF2) [Haemophilus parainfluenzae strain M1C149_1]
MKLIPINILSAVIFPFVFSACVSQSSVDFNKQQAAKARVELALGYLQQEDFVQAKLNLDKAFEHDEHYYLVHSALAHFYQ
LQGDTEKAKQAYLQAIKLDDKQGDVHNNFGAFLCGQGEFEQAYSQFNAALAAPNYYHQADTYENMALCAFAGKQTDVYQQ
ALDKLRQVDPSRAEKLRTFK

Nucleotide


Download         Length: 543 bp        

>NTDB_id=492964 INP98_RS09595 WP_197569998.1 1998979..1999521(-) (pilF/pilF2) [Haemophilus parainfluenzae strain M1C149_1]
ATGAAATTAATCCCAATTAACATTCTAAGTGCGGTCATTTTTCCTTTTGTTTTTTCGGCTTGTGTTTCTCAGTCTTCTGT
TGATTTTAATAAACAACAAGCAGCCAAAGCGCGTGTTGAATTGGCATTAGGCTATCTGCAACAAGAGGATTTTGTTCAAG
CAAAACTGAATTTAGATAAAGCCTTTGAACATGATGAACACTATTATCTTGTGCACTCGGCACTTGCGCATTTTTATCAA
TTACAAGGCGATACGGAAAAAGCGAAGCAAGCTTATTTACAGGCGATTAAGTTAGACGATAAGCAAGGTGATGTGCATAA
CAACTTCGGTGCTTTTTTATGTGGGCAAGGTGAGTTTGAACAAGCTTATTCACAATTTAATGCGGCACTCGCTGCACCGA
ATTATTATCATCAAGCTGATACTTACGAAAATATGGCACTTTGTGCTTTTGCCGGAAAACAAACCGATGTTTATCAACAA
GCGTTGGATAAATTACGCCAAGTTGATCCTTCTAGAGCGGAAAAGCTCCGCACGTTCAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(74-101)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

64.535

95.556

0.617
  pilF Glaesserella parasuis strain SC1401

48.889

100

0.489