Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   INP99_RS09620 Genome accession   NZ_CP063111
Coordinates   2005905..2006447 (-) Length   180 a.a.
NCBI ID   WP_197542295.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae strain M1C152_1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2000905..2011447
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP99_RS09595 (INP99_09585) - 2001266..2001709 (-) 444 WP_197542290.1 methyltransferase family protein -
  INP99_RS09600 (INP99_09590) - 2001762..2002376 (-) 615 WP_197542291.1 YfgM family protein -
  INP99_RS09605 (INP99_09595) hisS 2002395..2003666 (-) 1272 WP_197542292.1 histidine--tRNA ligase -
  INP99_RS09610 (INP99_09600) ispG 2003676..2004782 (-) 1107 WP_197542293.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  INP99_RS09615 (INP99_09605) - 2004801..2005829 (-) 1029 WP_197542294.1 RodZ domain-containing protein -
  INP99_RS09620 (INP99_09610) pilF/pilF2 2005905..2006447 (-) 543 WP_197542295.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  INP99_RS09625 (INP99_09615) - 2006517..2007665 (-) 1149 WP_197542296.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  INP99_RS09630 (INP99_09620) - 2007696..2007878 (+) 183 WP_054419666.1 hypothetical protein -
  INP99_RS09635 (INP99_09625) - 2007969..2008844 (+) 876 WP_054419668.1 D-alanyl-D-alanine carboxypeptidase family protein -
  INP99_RS09640 (INP99_09630) - 2008999..2009880 (+) 882 WP_049357006.1 metal ABC transporter substrate-binding protein -
  INP99_RS09645 (INP99_09635) - 2009880..2010776 (+) 897 WP_054419670.1 manganese/iron ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20420.07 Da        Isoelectric Point: 6.0902

>NTDB_id=492932 INP99_RS09620 WP_197542295.1 2005905..2006447(-) (pilF/pilF2) [Haemophilus parainfluenzae strain M1C152_1]
MKLIPINILSAVIFPFVFSACVSQSSVDFNKQQAAKARVELALGYLQQEDFVQAKLNLDKAFEHDERYYLVHSALAHFYQ
LQGDTEKVKQAYLQAIKLDDKQGDVYNNFGAFLCGQGEFEQAYSQFNAALAAPNYYHQADTYENIALCAFAGKQTDVYQQ
ALDKLRQVDPSRAEKLRTLK

Nucleotide


Download         Length: 543 bp        

>NTDB_id=492932 INP99_RS09620 WP_197542295.1 2005905..2006447(-) (pilF/pilF2) [Haemophilus parainfluenzae strain M1C152_1]
ATGAAATTAATCCCAATTAACATTCTAAGTGCGGTCATTTTTCCTTTTGTTTTTTCTGCTTGCGTCTCTCAGTCTTCTGT
TGATTTTAATAAACAACAAGCAGCAAAAGCGCGCGTTGAATTGGCATTAGGCTATCTGCAACAAGAGGATTTTGTTCAAG
CAAAACTTAACTTAGATAAAGCCTTTGAACATGATGAACGCTATTATCTTGTGCACTCGGCACTTGCGCATTTTTATCAA
TTACAAGGCGATACGGAAAAAGTGAAGCAAGCTTATTTACAGGCGATTAAGTTAGACGATAAGCAAGGTGATGTGTATAA
CAACTTTGGTGCATTTTTATGTGGGCAAGGTGAGTTTGAACAAGCTTATTCACAATTTAATGCGGCACTTGCAGCACCGA
ATTATTATCATCAAGCTGATACCTACGAAAACATAGCACTTTGTGCTTTTGCTGGAAAACAAACCGACGTTTATCAACAA
GCGTTGGATAAATTACGCCAAGTTGATCCTTCCAGAGCGGAAAAGCTCCGCACACTCAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

64.535

95.556

0.617
  pilF Glaesserella parasuis strain SC1401

49.153

98.333

0.483