Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   INQ00_RS09615 Genome accession   NZ_CP063110
Coordinates   2001317..2001859 (-) Length   180 a.a.
NCBI ID   WP_197546915.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae strain M1C160_1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1996317..2006859
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INQ00_RS09590 (INQ00_09590) - 1996680..1997123 (-) 444 WP_197546911.1 methyltransferase family protein -
  INQ00_RS09595 (INQ00_09595) - 1997174..1997788 (-) 615 WP_178411184.1 YfgM family protein -
  INQ00_RS09600 (INQ00_09600) hisS 1997807..1999078 (-) 1272 WP_197546912.1 histidine--tRNA ligase -
  INQ00_RS09605 (INQ00_09605) ispG 1999088..2000194 (-) 1107 WP_197546913.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  INQ00_RS09610 (INQ00_09610) - 2000213..2001241 (-) 1029 WP_197546914.1 RodZ domain-containing protein -
  INQ00_RS09615 (INQ00_09615) pilF/pilF2 2001317..2001859 (-) 543 WP_197546915.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  INQ00_RS09620 (INQ00_09620) - 2001929..2003077 (-) 1149 WP_049363743.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  INQ00_RS09625 (INQ00_09625) - 2003105..2003374 (+) 270 WP_178160845.1 hypothetical protein -
  INQ00_RS09630 (INQ00_09630) - 2003380..2004255 (+) 876 WP_049363744.1 D-alanyl-D-alanine carboxypeptidase family protein -
  INQ00_RS09635 (INQ00_09635) - 2004409..2005290 (+) 882 WP_065243887.1 metal ABC transporter substrate-binding protein -
  INQ00_RS09640 (INQ00_09640) - 2005290..2006192 (+) 903 WP_065243886.1 manganese/iron ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20397.98 Da        Isoelectric Point: 6.0902

>NTDB_id=492901 INQ00_RS09615 WP_197546915.1 2001317..2001859(-) (pilF/pilF2) [Haemophilus parainfluenzae strain M1C160_1]
MKLIPINILSAVIFSFVFSACVSQSSVDFNKQQAAKARVELALGYLQQEDFVQAKLNLDKAFEHDERYYLVHSALAHFYQ
LQGDTEKAKQAYLQSIKLDDKQGDVYNNFGAFLCGQGEFEQAYSQFNAALAAPNYYHQADTYENIALCAFAGKQTDVYQQ
ALDKLRQVDPSRAEKLRTLK

Nucleotide


Download         Length: 543 bp        

>NTDB_id=492901 INQ00_RS09615 WP_197546915.1 2001317..2001859(-) (pilF/pilF2) [Haemophilus parainfluenzae strain M1C160_1]
ATGAAATTAATCCCAATTAACATTCTAAGTGCGGTCATTTTTTCTTTTGTTTTTTCTGCTTGTGTTTCTCAGTCTTCTGT
TGATTTTAATAAACAACAAGCAGCAAAAGCCCGAGTTGAATTGGCATTAGGCTATCTGCAACAAGAGGATTTTGTTCAAG
CAAAACTGAATTTAGATAAAGCCTTTGAACATGATGAACGCTATTATCTTGTGCACTCGGCACTTGCGCATTTTTATCAA
TTACAAGGCGATACGGAAAAAGCGAAGCAAGCTTATTTACAGTCGATTAAGTTAGACGATAAGCAAGGTGATGTGTATAA
CAACTTTGGTGCATTTTTATGTGGGCAAGGTGAGTTTGAACAAGCTTATTCACAATTTAATGCGGCACTTGCAGCACCGA
ATTATTATCATCAAGCTGATACTTACGAAAACATAGCACTTTGTGCTTTTGCTGGAAAACAAACCGACGTTTATCAACAA
GCGTTGGATAAATTACGCCAAGTTGATCCTTCTAGAGCAGAAAAGCTCCGCACGCTCAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

63.953

95.556

0.611
  pilF Glaesserella parasuis strain SC1401

49.153

98.333

0.483