Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   M062_RS14190 Genome accession   NC_021577
Coordinates   3098715..3100442 (-) Length   575 a.a.
NCBI ID   WP_004350102.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa RP73     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3093715..3105442
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M062_RS14160 (M062_14180) - 3094575..3095654 (-) 1080 WP_003119955.1 general secretion pathway protein GspK -
  M062_RS14165 (M062_14185) - 3095644..3096234 (-) 591 WP_003119954.1 type II secretion system protein J -
  M062_RS14170 (M062_14190) - 3096231..3096656 (-) 426 WP_003116709.1 type II secretion system protein J -
  M062_RS14175 (M062_14195) - 3096656..3097066 (-) 411 WP_003097723.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  M062_RS14180 (M062_14200) gspG 3097035..3097469 (-) 435 WP_003110089.1 type II secretion system major pseudopilin GspG -
  M062_RS14185 (M062_14205) - 3097523..3098710 (-) 1188 WP_003097729.1 type II secretion system F family protein -
  M062_RS14190 (M062_14210) pilF 3098715..3100442 (-) 1728 WP_004350102.1 GspE/PulE family protein Machinery gene
  M062_RS14195 (M062_14215) - 3100432..3101235 (-) 804 WP_016263409.1 ABC transporter permease -
  M062_RS14200 (M062_14220) - 3101825..3102577 (-) 753 WP_016562021.1 class I SAM-dependent methyltransferase -
  M062_RS14205 (M062_14225) - 3102803..3103777 (+) 975 WP_003097735.1 quinone oxidoreductase family protein -
  M062_RS14210 (M062_14230) - 3103800..3104729 (+) 930 WP_003116706.1 LysR family transcriptional regulator -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63756.86 Da        Isoelectric Point: 5.3299

>NTDB_id=49260 M062_RS14190 WP_004350102.1 3098715..3100442(-) (pilF) [Pseudomonas aeruginosa RP73]
MSSEAVSPGPQMTAEWIPLGQRLLERGLVSGQELERALDLQRRLGGRLGGILVRSGAISENTLMQVLAEQLRLPLVGDDL
RKPSEESIAAFLLSTPINTDWFVEEQLVVWEEGEQLLFAARDPLSPSIRETLGYFYPERSIQAVLCRSQDLDGWLEHSLD
LTRQGADRHAYGSEDIRYLRELAEEAPVVELVNNVMSQAMEKRSSDIHIEPREFDFRIRFRVDGVLHDQLSLPRERFAAV
VSRIKLISAIDIAERRLPQDGRMSIRVSGTELDVRVSTLPGVHGESVVMRLLPKEREGLRLDRLGMLPDHLRRMRAWVAE
PHGIILVTGPTGSGKSTTLYGALEEINDGVRKLISVEDPVEYQVPNITQVQVHADIGLTFAAALRSILRQDPDVIMIGEI
RDLETAEIAVQSSLTGHLVLSTLHTNDAVSAFTRLIDMGVEPFLVASPVRGVQAQRLVRRLCAHCAEPCEPTLAAADLAA
TQAATARLFPGQAAQWRVARGCGRCEGTGYSGRVGIYELVDLSSEMRDLIVQRASLEQMHRLAASQGQRNLREDGLIKAW
LGMTSLDEVHRVTSG

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=49260 M062_RS14190 WP_004350102.1 3098715..3100442(-) (pilF) [Pseudomonas aeruginosa RP73]
ATGTCCTCTGAGGCCGTTTCCCCGGGCCCGCAGATGACGGCCGAGTGGATACCGCTGGGGCAGCGTTTGCTCGAACGCGG
GTTGGTGAGCGGCCAGGAACTCGAGCGGGCGCTCGACCTGCAACGCCGGCTTGGCGGCCGTCTGGGAGGCATTCTCGTGC
GTTCCGGAGCCATCTCGGAAAATACCCTCATGCAGGTGCTGGCGGAGCAACTGCGGTTGCCGCTGGTGGGGGACGACCTG
CGGAAGCCGAGCGAAGAGTCCATCGCCGCATTCCTGCTCAGTACCCCGATCAATACCGACTGGTTCGTCGAAGAGCAACT
GGTGGTCTGGGAGGAGGGCGAGCAACTGCTGTTCGCGGCGCGTGATCCGTTATCTCCTTCGATACGCGAAACCCTGGGCT
ATTTCTATCCGGAGCGCTCGATTCAAGCAGTGCTGTGCCGCTCCCAGGATCTGGACGGCTGGTTGGAGCACTCCCTGGAC
CTGACCCGGCAAGGTGCGGATCGGCACGCCTACGGCAGCGAAGACATCCGCTACCTGCGGGAGTTGGCGGAGGAGGCGCC
GGTAGTCGAACTCGTCAACAACGTGATGAGCCAGGCCATGGAAAAGCGTTCTTCGGATATTCACATCGAACCCCGGGAGT
TCGACTTCCGTATCCGCTTCCGCGTCGACGGCGTCCTGCACGACCAACTGAGCCTTCCCCGCGAGCGATTCGCGGCGGTC
GTCTCGCGGATCAAGCTGATCTCCGCCATCGACATCGCGGAGCGACGGTTGCCGCAGGATGGACGCATGAGCATCCGGGT
CAGCGGTACGGAGCTCGACGTGCGGGTCTCCACCTTGCCAGGGGTGCATGGCGAATCGGTGGTCATGCGCCTGTTGCCGA
AGGAGCGCGAGGGGTTGCGCCTGGACCGGCTCGGGATGCTCCCGGACCACTTGCGGAGGATGCGTGCATGGGTAGCGGAA
CCCCACGGCATCATCCTGGTGACCGGCCCCACCGGATCGGGCAAGTCCACCACGCTCTATGGCGCGCTGGAGGAAATCAA
TGATGGCGTGCGCAAGCTCATCAGCGTGGAAGATCCCGTGGAATACCAGGTGCCCAATATCACCCAGGTCCAGGTCCATG
CCGACATCGGCCTGACCTTCGCTGCCGCCTTGCGCTCGATCCTGCGGCAGGATCCCGATGTGATCATGATCGGGGAGATA
CGCGACCTGGAGACGGCGGAGATCGCCGTACAGTCCTCGCTCACCGGTCACCTGGTGCTTTCGACCCTGCATACCAACGA
TGCGGTGAGCGCCTTCACGCGTTTGATCGACATGGGCGTGGAACCCTTCCTGGTGGCTTCGCCGGTGCGCGGTGTCCAGG
CCCAGCGACTGGTGCGCAGGTTGTGCGCCCACTGCGCGGAACCTTGCGAGCCAACCCTTGCTGCGGCCGATCTGGCAGCG
ACCCAGGCGGCCACCGCGCGGCTGTTCCCGGGGCAGGCAGCGCAATGGCGCGTGGCGCGAGGCTGCGGCCGCTGCGAAGG
TACCGGCTACAGCGGCCGTGTGGGCATCTACGAACTCGTCGATCTGTCTTCCGAGATGCGCGACCTGATCGTGCAGAGAG
CTTCCCTCGAACAGATGCATCGGCTTGCCGCCAGCCAGGGACAGCGCAATCTGCGTGAAGATGGCTTGATCAAGGCCTGG
TTGGGTATGACCAGTCTTGACGAGGTGCATCGCGTGACCAGCGGCTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-159)

Type II/IV secretion system protein (T2SSE)

(194-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

41.343

98.435

0.407
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.184

98.087

0.384
  pilF Neisseria gonorrhoeae MS11

36.842

99.13

0.365

Multiple sequence alignment