Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   IHV77_RS02440 Genome accession   NZ_CP063056
Coordinates   494855..495403 (-) Length   182 a.a.
NCBI ID   WP_194812576.1    Uniprot ID   -
Organism   Rodentibacter haemolyticus strain DSM 111151     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 489855..500403
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHV77_RS02415 (IHV77_02415) - 490277..490720 (-) 444 WP_408635273.1 methyltransferase family protein -
  IHV77_RS02420 (IHV77_02420) - 490751..491365 (-) 615 WP_194812573.1 YfgM family protein -
  IHV77_RS02425 (IHV77_02425) hisS 491384..492655 (-) 1272 WP_194812574.1 histidine--tRNA ligase -
  IHV77_RS02430 (IHV77_02430) ispG 492665..493771 (-) 1107 WP_194812575.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  IHV77_RS02435 (IHV77_02435) - 493783..494787 (-) 1005 WP_408635274.1 RodZ domain-containing protein -
  IHV77_RS02440 (IHV77_02440) pilF/pilF2 494855..495403 (-) 549 WP_194812576.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  IHV77_RS02445 (IHV77_02445) - 495430..496563 (-) 1134 WP_194812577.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  IHV77_RS02450 (IHV77_02450) - 496797..497672 (+) 876 WP_194812578.1 D-alanyl-D-alanine carboxypeptidase family protein -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20947.45 Da        Isoelectric Point: 7.0375

>NTDB_id=492592 IHV77_RS02440 WP_194812576.1 494855..495403(-) (pilF/pilF2) [Rodentibacter haemolyticus strain DSM 111151]
MKPFFIQYLSAVIFPLIFSACVSQTSSSDDFNKQQAAKARVELALGYLHHNNFPQAKQNLDKALEHDKNYSLVHSAFAHF
YQLQGDVISARESYLQAIKLNDKQGETYNNFGAFLCGQGEFEQARQAFEQALSSPRYYHQADTYENLALCARAFNQKDIY
QQAVDKLRQIDEYRAEKLNQIK

Nucleotide


Download         Length: 549 bp        

>NTDB_id=492592 IHV77_RS02440 WP_194812576.1 494855..495403(-) (pilF/pilF2) [Rodentibacter haemolyticus strain DSM 111151]
ATGAAACCCTTCTTTATTCAATACTTAAGTGCGGTCATTTTTCCTCTTATTTTTTCAGCCTGTGTTTCTCAAACTTCTTC
CTCTGATGATTTCAATAAACAGCAAGCGGCAAAAGCTCGCGTGGAATTAGCCTTAGGTTATCTGCATCACAACAATTTTC
CTCAAGCTAAACAGAATTTGGATAAGGCCTTGGAACATGATAAAAATTACTCTCTTGTGCATTCTGCATTTGCACATTTC
TATCAGCTACAAGGTGATGTAATCTCCGCGCGGGAATCTTATCTTCAAGCAATAAAACTTAATGATAAACAAGGCGAAAC
CTATAATAATTTCGGTGCCTTTTTATGTGGGCAGGGAGAATTTGAACAAGCCCGTCAAGCCTTTGAACAGGCATTATCCT
CACCTCGTTATTATCACCAAGCCGATACTTATGAAAATCTGGCGCTTTGCGCACGAGCATTCAACCAAAAGGATATTTAT
CAACAAGCTGTCGATAAATTACGGCAGATCGATGAATATCGTGCGGAAAAACTGAATCAAATCAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(77-103)

Tetratricopeptide repeat (TPR_10)

(107-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

64.943

95.604

0.621
  pilF Glaesserella parasuis strain SC1401

47.283

100

0.478