Detailed information    

insolico Bioinformatically predicted

Overview

Name   uvrC   Type   Machinery gene
Locus tag   KIM43_RS12320 Genome accession   NZ_CP074773
Coordinates   2405045..2406877 (+) Length   610 a.a.
NCBI ID   WP_001283424.1    Uniprot ID   B1IZZ1
Organism   Escherichia coli strain 97_3250     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2400045..2411877
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIM43_RS12280 (KIM43_12155) dcyD 2400271..2401257 (+) 987 WP_001128238.1 D-cysteine desulfhydrase -
  KIM43_RS12285 (KIM43_12160) tcyL 2401272..2401940 (+) 669 WP_001158220.1 cystine ABC transporter permease -
  KIM43_RS12290 (KIM43_12165) tcyN 2401937..2402689 (+) 753 WP_001272994.1 L-cystine ABC transporter ATP-binding protein TcyN -
  KIM43_RS12295 (KIM43_12170) sdiA 2402919..2403641 (+) 723 WP_001154265.1 transcriptional regulator SdiA -
  KIM43_RS12300 (KIM43_12175) yecF 2403709..2403933 (-) 225 WP_000106474.1 DUF2594 family protein YecF -
  KIM43_RS12305 (KIM43_12180) yecU 2403920..2404096 (-) 177 WP_001342215.1 protein YecU -
  KIM43_RS12310 - 2404134..2404250 (-) 117 WP_001302039.1 hypothetical protein -
  KIM43_RS12315 (KIM43_12185) letA 2404392..2405048 (+) 657 WP_000611328.1 UvrY/SirA/GacA family response regulator transcription factor Regulator
  KIM43_RS12320 (KIM43_12190) uvrC 2405045..2406877 (+) 1833 WP_001283424.1 excinuclease ABC subunit UvrC Machinery gene
  KIM43_RS12325 (KIM43_12195) pgsA 2406934..2407482 (+) 549 WP_001160187.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
  KIM43_RS12345 (KIM43_12215) - 2408475..2408756 (+) 282 WP_001303543.1 hypothetical protein -
  KIM43_RS12350 (KIM43_12220) - 2408945..2409085 (-) 141 WP_000078921.1 Hok/Gef family protein -
  KIM43_RS12355 (KIM43_12225) - 2409276..2409536 (-) 261 WP_000488107.1 ogr/Delta-like zinc finger family protein -
  KIM43_RS12360 (KIM43_12230) - 2409823..2410962 (-) 1140 WP_001317900.1 YecA family protein -

Sequence

Protein


Download         Length: 610 a.a.        Molecular weight: 68216.09 Da        Isoelectric Point: 9.3685

>NTDB_id=492417 KIM43_RS12320 WP_001283424.1 2405045..2406877(+) (uvrC) [Escherichia coli strain 97_3250]
MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLSSYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLL
EHNYIKLYQPRYNVLLRDDKSYPFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETASQNLEFEEAARIRDQIQAVRRVT
EKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKVLGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLD
FNLSDKTLLADSLSELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEVKRMECFDI
SHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRYGKAIDDSKIPDVILIDGGKGQLAQAKNVFA
ELDVSWDKNHPLLLGVAKGVDRKAGLETLFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLET
IEGVGPKRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH

Nucleotide


Download         Length: 1833 bp        

>NTDB_id=492417 KIM43_RS12320 WP_001283424.1 2405045..2406877(+) (uvrC) [Escherichia coli strain 97_3250]
GTGAGTGATCAGTTTGACGCAAAAGCGTTTTTAAAAACCGTAACCAGCCAGCCAGGCGTTTATCGCATGTACGATGCTGG
TGGTACGGTTATCTATGTCGGCAAAGCGAAAGACCTGAAAAAACGGCTTTCCAGCTATTTCCGTAGCAACCTCGCTTCGC
GCAAAACCGAAGCGCTGGTCGCCCAGATCCAGCAAATTGATGTAACGGTTACTCATACAGAAACCGAAGCGCTGTTGCTG
GAACACAACTACATCAAACTCTATCAGCCGCGTTACAACGTTTTGCTACGCGATGATAAATCTTATCCTTTTATCTTCCT
GAGTGGCGATACCCACCCGCGTCTGGCGATGCATCGTGGTGCGAAGCATGCCAAAGGTGAATATTTCGGCCCGTTCCCGA
ATGGCTATGCCGTACGTGAAACACTGGCGCTACTGCAAAAGATTTTCCCCATTCGCCAGTGCGAAAATAGTGTTTATCGC
AATCGCTCGCGTCCGTGTCTGCAATACCAGATAGGGCGCTGTCTGGGACCGTGCGTTGAAGGACTGGTGAGTGAAGAAGA
ATACGCTCAGCAGGTCGAGTATGTGCGCCTGTTTTTGTCTGGCAAAGATGATCAGGTGCTTACGCAACTCATTAGTCGTA
TGGAAACTGCCAGCCAGAATCTGGAGTTTGAAGAAGCTGCACGTATTCGCGACCAAATTCAGGCGGTGCGACGCGTCACC
GAAAAACAATTCGTTTCCAATACCGGCGACGACCTCGACGTTATTGGTGTGGCGTTCGATGCGGGCATGGCTTGTGTCCA
CGTATTGTTCATTCGTCAGGGCAAAGTGCTCGGCAGCCGCAGCTATTTCCCGAAAGTGCCTGGCGGTACGGAACTGAGCG
AGGTGGTAGAAACCTTCGTAGGCCAGTTCTATTTACAAGGCAGCCAGATGCGCACCTTACCGGGTGAGATCCTGCTCGAT
TTTAATCTTAGCGATAAAACGCTGCTCGCCGATTCCCTTTCAGAACTGGCGGGACGCAAGATTAATGTTCAAACCAAACC
TCGCGGCGATAGGGCGCGTTATCTGAAACTCGCGCGCACCAATGCGGCGACGGCCTTAACCAGCAAACTTTCGCAGCAAT
CTACCGTTCACCAGCGACTGACCGCGCTTGCCAGCGTGTTGAAATTGCCGGAAGTGAAGCGGATGGAGTGCTTTGACATC
AGCCATACCATGGGCGAACAAACCGTCGCTTCCTGTGTGGTGTTTGATGCTAACGGCCCGCTGCGTGCGGAGTATCGGCG
CTATAACATTACAGGCATCACGCCGGGCGATGATTATGCGGCGATGAATCAGGTGCTGCGTCGGCGTTATGGTAAAGCCA
TTGACGACAGTAAGATCCCGGATGTGATCCTTATCGACGGCGGCAAAGGCCAGCTTGCGCAGGCGAAAAATGTCTTCGCC
GAACTGGATGTCTCATGGGATAAAAATCATCCACTGCTACTTGGCGTTGCCAAAGGAGTAGATCGTAAGGCTGGGCTGGA
AACACTGTTCTTTGAGCCGGAAGGTGAGGGATTCAGTTTGCCGCCAGATTCTCCCGCGCTGCATGTTATCCAGCATATTC
GCGATGAATCACACGATCACGCGATTGGCGGGCACCGTAAAAAACGGGCGAAGGTCAAAAATACCAGCTCCCTGGAAACC
ATTGAAGGCGTCGGGCCAAAACGTCGGCAAATGTTGTTGAAATATATGGGCGGTTTGCAAGGTTTACGTAACGCCAGCGT
CGAGGAAATTGCAAAAGTGCCGGGTATTTCGCAAGGTCTGGCAGAAAAGATCTTCTGGTCGTTGAAACATTGA

Domains

Predicted by InterProScan.

UvrB/uvrC motif (UVR)

(205-237)

Helix-hairpin-helix domain (HHH_5)

(558-609)

GIY-YIG catalytic domain (GIY-YIG)

(18-93)

UvrC RNAse H endonuclease domain (UvrC_RNaseH_dom)

(385-542)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B1IZZ1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  uvrC Streptococcus pneumoniae TIGR4

37.891

99.508

0.377
  uvrC Streptococcus pneumoniae R6

37.562

99.508

0.374
  uvrC Streptococcus pneumoniae D39

37.562

99.508

0.374