Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   TTJL18_RS03340 Genome accession   NC_017587
Coordinates   589727..590860 (-) Length   377 a.a.
NCBI ID   WP_014629296.1    Uniprot ID   H9ZQG4
Organism   Thermus thermophilus JL-18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 584727..595860
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TTJL18_RS03320 (TtJL18_0665) pilQ 585431..587698 (-) 2268 WP_014629292.1 secretin N-terminal domain-containing protein Machinery gene
  TTJL18_RS03325 (TtJL18_0666) pilW 587695..588543 (-) 849 WP_014629293.1 hypothetical protein Machinery gene
  TTJL18_RS03330 (TtJL18_0667) pilO 588540..589121 (-) 582 WP_014629294.1 type 4a pilus biogenesis protein PilO Machinery gene
  TTJL18_RS03335 (TtJL18_0668) pilN 589111..589734 (-) 624 WP_014629295.1 competence protein Machinery gene
  TTJL18_RS03340 (TtJL18_0669) pilM 589727..590860 (-) 1134 WP_014629296.1 type IV pilus assembly protein PilM Machinery gene
  TTJL18_RS03345 (TtJL18_0670) - 590990..591994 (-) 1005 WP_014629297.1 homoisocitrate dehydrogenase -
  TTJL18_RS03350 (TtJL18_0671) - 592049..592540 (-) 492 WP_011173430.1 DUF5317 domain-containing protein -
  TTJL18_RS03355 (TtJL18_0672) - 592591..594027 (-) 1437 WP_049793115.1 HD domain-containing phosphohydrolase -
  TTJL18_RS03370 (TtJL18_0675) - 594896..595261 (+) 366 WP_014629299.1 hypothetical protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41377.99 Da        Isoelectric Point: 4.3557

>NTDB_id=49120 TTJL18_RS03340 WP_014629296.1 589727..590860(-) (pilM) [Thermus thermophilus JL-18]
MFKSLSQLFRPRVEALGLEIGASALKLVEVSGNPPALKALASRPTPPGLLVEGMVAEPAALAEEIKELLLEARTRKRYVV
TALSNLAVILRPIQVPKMPLKEMEEAVRWEAERYIPFPIDEVVLDFAPLTPLSEVQEGEQVQVMVAAARQEAVAGVLEAL
RGAGLVPVVLDVKPFAGLYPLEAKLAEDPDRVFLVLDIGAESTSLVLLRGDKPLAVRILTLSGKDFTEAIAQSFNLDLLA
AEEVKRTYGMATLPTEDEELLLDFDAERERYSPGRIYDAIRPVLVELTQELRRSLEFFRIQLEEASPEMGYLLGGGSKLR
GLASLLTDTLGVNFEPVNPWEAVTVDPKRFEAEKLQEMGPEFAVALGLALRGVEPFD

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=49120 TTJL18_RS03340 WP_014629296.1 589727..590860(-) (pilM) [Thermus thermophilus JL-18]
GTGTTCAAAAGCCTTAGCCAGCTCTTCCGACCCAGGGTTGAGGCCCTGGGGTTGGAGATCGGGGCCTCCGCCCTGAAGCT
CGTGGAGGTGTCCGGGAACCCCCCTGCCCTCAAGGCCCTGGCCTCCCGCCCCACCCCGCCCGGCCTCCTGGTGGAAGGGA
TGGTGGCCGAGCCCGCCGCCTTAGCCGAGGAGATCAAGGAGCTTCTCCTCGAGGCCCGCACCCGCAAGCGCTACGTGGTC
ACCGCCCTCTCCAACCTGGCGGTCATCCTCCGCCCCATCCAGGTTCCCAAGATGCCCCTCAAGGAGATGGAGGAGGCGGT
GCGCTGGGAGGCGGAGCGCTACATCCCCTTCCCCATTGACGAGGTGGTCCTGGACTTCGCCCCCCTGACCCCCCTCTCCG
AGGTGCAGGAGGGGGAGCAGGTCCAGGTGATGGTGGCGGCGGCGCGGCAGGAGGCGGTGGCGGGGGTCCTCGAGGCCTTG
AGGGGGGCGGGGCTCGTCCCCGTGGTGCTGGACGTGAAGCCCTTCGCCGGGCTCTACCCCCTGGAGGCCAAGCTTGCGGA
GGACCCCGACCGGGTCTTCCTCGTTCTGGACATTGGGGCCGAGAGCACAAGCCTCGTCCTGCTCCGGGGCGATAAGCCTT
TGGCGGTGCGCATCCTGACCCTCTCGGGCAAGGACTTCACCGAGGCCATCGCGCAAAGTTTCAATCTGGACCTCCTCGCC
GCCGAGGAGGTGAAGCGGACCTACGGGATGGCCACCCTCCCCACCGAGGACGAGGAGCTCCTCCTGGACTTTGACGCCGA
GCGGGAGCGCTACAGCCCGGGGCGCATCTATGACGCCATCCGCCCGGTGCTGGTGGAGCTCACTCAGGAGCTCCGCCGTA
GCCTTGAGTTCTTCCGCATCCAGCTGGAGGAGGCCTCGCCCGAGATGGGCTACCTCCTGGGCGGGGGGAGCAAGCTCAGG
GGGCTCGCCTCCCTCCTCACCGACACCCTGGGGGTGAACTTTGAGCCCGTCAACCCCTGGGAGGCGGTAACGGTGGACCC
CAAGCGCTTTGAGGCGGAGAAGCTCCAGGAGATGGGGCCCGAGTTCGCCGTAGCCCTGGGCCTGGCCCTGAGGGGGGTGG
AGCCCTTTGATTAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-372)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB H9ZQG4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Thermus thermophilus HB27

97.082

100

0.971

Multiple sequence alignment