Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   ILP78_RS07025 Genome accession   NZ_CP062465
Coordinates   1388666..1389163 (+) Length   165 a.a.
NCBI ID   WP_029694050.1    Uniprot ID   -
Organism   Staphylococcus aureus strain MRSA - AMRF 6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1383666..1394163
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ILP78_RS06985 (ILP78_06985) - 1383710..1384696 (+) 987 WP_000161315.1 ROK family glucokinase -
  ILP78_RS06990 (ILP78_06990) - 1384696..1385025 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  ILP78_RS06995 (ILP78_06995) - 1385022..1385645 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  ILP78_RS07000 (ILP78_07000) comGA 1385697..1386671 (+) 975 WP_000697219.1 competence type IV pilus ATPase ComGA Machinery gene
  ILP78_RS07005 (ILP78_07005) comGB 1386643..1387713 (+) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  ILP78_RS07010 (ILP78_07010) comGC 1387727..1388038 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  ILP78_RS07015 (ILP78_07015) comGD 1388016..1388462 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  ILP78_RS07020 (ILP78_07020) comGE 1388449..1388748 (+) 300 WP_000844410.1 hypothetical protein Machinery gene
  ILP78_RS07025 (ILP78_07025) comGF 1388666..1389163 (+) 498 WP_029694050.1 competence type IV pilus minor pilin ComGF Machinery gene
  ILP78_RS07030 (ILP78_07030) - 1389260..1389406 (+) 147 WP_001789879.1 hypothetical protein -
  ILP78_RS07035 (ILP78_07035) - 1389396..1389920 (+) 525 WP_001015121.1 shikimate kinase -
  ILP78_RS07040 (ILP78_07040) gcvT 1390079..1391170 (+) 1092 WP_000093345.1 glycine cleavage system aminomethyltransferase GcvT -
  ILP78_RS07045 (ILP78_07045) gcvPA 1391190..1392536 (+) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  ILP78_RS07050 (ILP78_07050) gcvPB 1392529..1394001 (+) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19199.92 Da        Isoelectric Point: 10.2192

>NTDB_id=490207 ILP78_RS07025 WP_029694050.1 1388666..1389163(+) (comGF) [Staphylococcus aureus strain MRSA - AMRF 6]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIELRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTSFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=490207 ILP78_RS07025 WP_029694050.1 1388666..1389163(+) (comGF) [Staphylococcus aureus strain MRSA - AMRF 6]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAACTTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CTTCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(68-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

96.97

100

0.97
  comGF Staphylococcus aureus N315

96.97

100

0.97