Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   IF720_RS02755 Genome accession   NZ_CP062415
Coordinates   499948..500445 (+) Length   165 a.a.
NCBI ID   WP_001794055.1    Uniprot ID   A0A7U7EV69
Organism   Staphylococcus aureus strain NAS_AN_150     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 494948..505445
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF720_RS02715 (IF720_02720) - 494992..495978 (+) 987 WP_000161314.1 ROK family glucokinase -
  IF720_RS02720 (IF720_02725) - 495978..496307 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  IF720_RS02725 (IF720_02730) - 496304..496927 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  IF720_RS02730 (IF720_02735) comGA 496979..497953 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  IF720_RS02735 (IF720_02740) comGB 497925..498995 (+) 1071 WP_000776412.1 competence type IV pilus assembly protein ComGB Machinery gene
  IF720_RS02740 (IF720_02745) comGC 499009..499320 (+) 312 WP_000472257.1 competence type IV pilus major pilin ComGC Machinery gene
  IF720_RS02745 (IF720_02750) comGD 499298..499744 (+) 447 WP_001791207.1 competence type IV pilus minor pilin ComGD Machinery gene
  IF720_RS02750 (IF720_02755) comGE 499731..500030 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  IF720_RS02755 (IF720_02760) comGF 499948..500445 (+) 498 WP_001794055.1 competence type IV pilus minor pilin ComGF Machinery gene
  IF720_RS02760 (IF720_02765) - 500542..500688 (+) 147 WP_001789879.1 hypothetical protein -
  IF720_RS02765 (IF720_02770) - 500678..501202 (+) 525 WP_001015125.1 shikimate kinase -
  IF720_RS02770 (IF720_02775) gcvT 501361..502452 (+) 1092 WP_000093347.1 glycine cleavage system aminomethyltransferase GcvT -
  IF720_RS02775 (IF720_02780) gcvPA 502472..503818 (+) 1347 WP_000019697.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  IF720_RS02780 (IF720_02785) gcvPB 503811..505283 (+) 1473 WP_000202178.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19224.98 Da        Isoelectric Point: 10.2192

>NTDB_id=489866 IF720_RS02755 WP_001794055.1 499948..500445(+) (comGF) [Staphylococcus aureus strain NAS_AN_150]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLNKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=489866 IF720_RS02755 WP_001794055.1 499948..500445(+) (comGF) [Staphylococcus aureus strain NAS_AN_150]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTAATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAACAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7U7EV69

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

96.97

100

0.97