Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   IF729_RS02400 Genome accession   NZ_CP062410
Coordinates   445043..445540 (+) Length   165 a.a.
NCBI ID   WP_001788897.1    Uniprot ID   -
Organism   Staphylococcus aureus strain NAS_AN_205     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 440043..450540
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF729_RS02360 (IF729_02350) - 440087..441073 (+) 987 WP_000161311.1 ROK family glucokinase -
  IF729_RS02365 (IF729_02355) - 441073..441402 (+) 330 WP_240053137.1 MTH1187 family thiamine-binding protein -
  IF729_RS02370 (IF729_02360) - 441399..442022 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  IF729_RS02375 (IF729_02365) comGA 442074..443048 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  IF729_RS02380 (IF729_02370) comGB 443020..444090 (+) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  IF729_RS02385 (IF729_02375) comGC 444104..444415 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  IF729_RS02390 (IF729_02380) comGD 444393..444839 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  IF729_RS02395 (IF729_02385) comGE 444826..445125 (+) 300 WP_000844410.1 hypothetical protein Machinery gene
  IF729_RS02400 (IF729_02390) comGF 445043..445540 (+) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  IF729_RS02405 (IF729_02395) - 445637..445783 (+) 147 WP_001792109.1 hypothetical protein -
  IF729_RS02410 (IF729_02400) - 445773..446297 (+) 525 WP_001015120.1 shikimate kinase -
  IF729_RS02415 (IF729_02405) gcvT 446456..447547 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  IF729_RS02420 (IF729_02410) gcvPA 447567..448913 (+) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  IF729_RS02425 (IF729_02415) gcvPB 448906..450378 (+) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19181.99 Da        Isoelectric Point: 10.1964

>NTDB_id=489653 IF729_RS02400 WP_001788897.1 445043..445540(+) (comGF) [Staphylococcus aureus strain NAS_AN_205]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=489653 IF729_RS02400 WP_001788897.1 445043..445540(+) (comGF) [Staphylococcus aureus strain NAS_AN_205]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

100

100

1
  comGF Staphylococcus aureus MW2

96.364

100

0.964