Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KFU84_RS11550 Genome accession   NZ_CP073981
Coordinates   2405510..2406319 (+) Length   269 a.a.
NCBI ID   WP_001300405.1    Uniprot ID   -
Organism   Escherichia coli strain MB19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2400510..2411319
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU84_RS11545 (KFU84_11430) sslE 2400750..2405312 (+) 4563 WP_032181764.1 lipoprotein metalloprotease SslE -
  KFU84_RS11550 (KFU84_11435) pilD 2405510..2406319 (+) 810 WP_001300405.1 prepilin peptidase PppA Machinery gene
  KFU84_RS11555 (KFU84_11440) gspS2 2406385..2406795 (+) 411 WP_001300497.1 type II secretion system pilot lipoprotein GspS-beta -
  KFU84_RS11560 (KFU84_11445) gspC 2406813..2407772 (+) 960 WP_000135079.1 type II secretion system protein GspC -
  KFU84_RS11565 (KFU84_11450) - 2407773..2408006 (+) 234 Protein_2257 type II secretion system protein GspG -
  KFU84_RS11570 (KFU84_11455) gspH 2408010..2408573 (+) 564 WP_001115154.1 type II secretion system minor pseudopilin GspH -
  KFU84_RS11575 (KFU84_11460) gspI 2408570..2408941 (+) 372 WP_000820113.1 type II secretion system minor pseudopilin GspI -
  KFU84_RS11580 (KFU84_11465) gspJ 2408938..2409537 (+) 600 WP_001255034.1 type II secretion system minor pseudopilin GspJ -
  KFU84_RS11585 (KFU84_11470) gspK 2409540..2410517 (+) 978 WP_000633209.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 29480.04 Da        Isoelectric Point: 8.3829

>NTDB_id=489264 KFU84_RS11550 WP_001300405.1 2405510..2406319(+) (pilD) [Escherichia coli strain MB19]
MLFDVFQQYPTAMPVLATVGGLIIGSFLNVVIWRYPIMLRQQMAEFHGEMSSAQSKISLALPRSHCPHCQQTIRIRDNIP
LFSWLMLKGRCRDCQAKISKRYPLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDHQWLPDVFTQGVLWT
GLSAAWAQQSPLTLQDAVTGVLVGFIAFYSLRWIAGIVLRKEALGMGDVLLFAALGSWVGPLSLPNVALIASCCGLIYAV
ITKRGSTTLPFGPCLSLGGIATIYLQALF

Nucleotide


Download         Length: 810 bp        

>NTDB_id=489264 KFU84_RS11550 WP_001300405.1 2405510..2406319(+) (pilD) [Escherichia coli strain MB19]
ATGCTTTTTGATGTTTTTCAGCAATACCCCACGGCGATGCCCGTCCTGGCAACCGTCGGAGGATTGATCATCGGCAGTTT
TTTGAATGTGGTGATTTGGCGTTACCCAATCATGCTGCGCCAACAAATGGCGGAGTTTCACGGTGAAATGTCGAGTGCGC
AGTCAAAAATAAGCCTTGCGCTACCGCGTTCGCACTGCCCACATTGTCAGCAGACCATCCGGATACGTGACAATATTCCG
CTGTTCTCCTGGCTGATGCTCAAAGGGCGCTGCCGCGACTGTCAGGCGAAAATCAGCAAGCGTTATCCGCTGGTGGAGTT
ATTGACGGCACTCGCTTTTTTGCTGGCGAGTCTGGTCTGGCCGGAAAGTGGATGGGCGCTGGCGGTGATGATATTATCCG
CCTGGCTGATTGCCGCGAGCGTCATTGACCTCGATCACCAATGGCTGCCCGATGTTTTTACTCAGGGCGTATTGTGGACG
GGACTGAGTGCGGCATGGGCGCAGCAGAGCCCGCTCACGCTACAAGATGCAGTTACCGGCGTCCTGGTGGGGTTTATCGC
TTTTTACTCCCTGCGCTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTATTGTTCGCTG
CGTTAGGTAGTTGGGTGGGGCCGTTGTCGCTACCCAATGTTGCTTTAATCGCATCATGCTGCGGCCTGATATATGCCGTT
ATTACAAAAAGAGGATCAACCACACTGCCTTTTGGACCGTGTTTAAGTCTGGGCGGTATAGCAACAATTTATCTACAGGC
ATTGTTTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(130-239)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

45.318

99.257

0.45
  pilD Vibrio campbellii strain DS40M4

42.697

99.257

0.424
  pilD Acinetobacter nosocomialis M2

38.491

98.513

0.379
  pilD Acinetobacter baumannii D1279779

38.113

98.513

0.375
  pilD Neisseria gonorrhoeae MS11

38.911

95.539

0.372