Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   IF694_RS02325 Genome accession   NZ_CP062395
Coordinates   439297..439794 (+) Length   165 a.a.
NCBI ID   WP_001825340.1    Uniprot ID   A0A2S6DDC2
Organism   Staphylococcus aureus strain NAS_AN_005     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 434297..444794
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF694_RS02285 (IF694_02260) - 434341..435327 (+) 987 WP_000161315.1 ROK family glucokinase -
  IF694_RS02290 (IF694_02265) - 435327..435656 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  IF694_RS02295 (IF694_02270) - 435653..436276 (+) 624 WP_047213494.1 MBL fold metallo-hydrolase -
  IF694_RS02300 (IF694_02275) comGA 436328..437302 (+) 975 WP_000697222.1 competence type IV pilus ATPase ComGA Machinery gene
  IF694_RS02305 (IF694_02280) comGB 437274..438344 (+) 1071 WP_000776413.1 competence type IV pilus assembly protein ComGB Machinery gene
  IF694_RS02310 (IF694_02285) comGC 438358..438669 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  IF694_RS02315 (IF694_02290) comGD 438647..439093 (+) 447 WP_072357807.1 competence type IV pilus minor pilin ComGD Machinery gene
  IF694_RS02320 (IF694_02295) comGE 439080..439379 (+) 300 WP_000844415.1 hypothetical protein Machinery gene
  IF694_RS02325 (IF694_02300) comGF 439297..439794 (+) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  IF694_RS02330 (IF694_02305) - 439891..440037 (+) 147 WP_001789879.1 hypothetical protein -
  IF694_RS02335 (IF694_02310) - 440027..440551 (+) 525 WP_001015120.1 shikimate kinase -
  IF694_RS02340 (IF694_02315) gcvT 440710..441801 (+) 1092 WP_000093354.1 glycine cleavage system aminomethyltransferase GcvT -
  IF694_RS02345 (IF694_02320) gcvPA 441821..443167 (+) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  IF694_RS02350 (IF694_02325) gcvPB 443160..444632 (+) 1473 WP_000202177.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19179.92 Da        Isoelectric Point: 10.2192

>NTDB_id=489111 IF694_RS02325 WP_001825340.1 439297..439794(+) (comGF) [Staphylococcus aureus strain NAS_AN_005]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=489111 IF694_RS02325 WP_001825340.1 439297..439794(+) (comGF) [Staphylococcus aureus strain NAS_AN_005]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S6DDC2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

96.97

100

0.97