Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   IF703_RS02420 Genome accession   NZ_CP062382
Coordinates   457604..458578 (+) Length   324 a.a.
NCBI ID   WP_000697222.1    Uniprot ID   A0A2S6D0Y6
Organism   Staphylococcus aureus strain NAS_AN_068     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 452604..463578
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF703_RS02385 (IF703_02370) rpmG 453064..453213 (+) 150 WP_001265709.1 50S ribosomal protein L33 -
  IF703_RS02390 (IF703_02375) - 453422..453961 (+) 540 WP_000164552.1 5-formyltetrahydrofolate cyclo-ligase -
  IF703_RS02395 (IF703_02380) - 453973..455436 (+) 1464 WP_001017867.1 rhomboid family intramembrane serine protease -
  IF703_RS02400 (IF703_02385) - 455417..455620 (+) 204 WP_000087561.1 YqgQ family protein -
  IF703_RS02405 (IF703_02390) - 455617..456603 (+) 987 WP_000161315.1 ROK family glucokinase -
  IF703_RS02410 (IF703_02395) - 456603..456932 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  IF703_RS02415 (IF703_02400) - 456929..457552 (+) 624 WP_047213494.1 MBL fold metallo-hydrolase -
  IF703_RS02420 (IF703_02405) comGA 457604..458578 (+) 975 WP_000697222.1 competence type IV pilus ATPase ComGA Machinery gene
  IF703_RS02425 (IF703_02410) comGB 458550..459620 (+) 1071 Protein_446 competence type IV pilus assembly protein ComGB -
  IF703_RS02430 (IF703_02415) comGC 459634..459945 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  IF703_RS02435 (IF703_02420) comGD 459923..460369 (+) 447 WP_072357807.1 competence type IV pilus minor pilin ComGD Machinery gene
  IF703_RS02440 (IF703_02425) comGE 460356..460655 (+) 300 WP_000844415.1 hypothetical protein Machinery gene
  IF703_RS02445 (IF703_02430) comGF 460573..461070 (+) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  IF703_RS02450 (IF703_02435) - 461167..461313 (+) 147 WP_001789879.1 hypothetical protein -
  IF703_RS02455 (IF703_02440) - 461303..461827 (+) 525 WP_001015120.1 shikimate kinase -
  IF703_RS02460 (IF703_02445) gcvT 461986..463077 (+) 1092 WP_000093354.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36874.92 Da        Isoelectric Point: 8.8508

>NTDB_id=488859 IF703_RS02420 WP_000697222.1 457604..458578(+) (comGA) [Staphylococcus aureus strain NAS_AN_068]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSNNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=488859 IF703_RS02420 WP_000697222.1 457604..458578(+) (comGA) [Staphylococcus aureus strain NAS_AN_068]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTAATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGACCTACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAAGATCCTGTAGAGATGCAAATTCCTGGTATTGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCAATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S6D0Y6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.691

100

0.997
  comGA Staphylococcus aureus N315

99.691

100

0.997