Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   IF703_RS02445 Genome accession   NZ_CP062382
Coordinates   460573..461070 (+) Length   165 a.a.
NCBI ID   WP_001825340.1    Uniprot ID   A0A2S6DDC2
Organism   Staphylococcus aureus strain NAS_AN_068     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 455573..466070
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF703_RS02405 (IF703_02390) - 455617..456603 (+) 987 WP_000161315.1 ROK family glucokinase -
  IF703_RS02410 (IF703_02395) - 456603..456932 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  IF703_RS02415 (IF703_02400) - 456929..457552 (+) 624 WP_047213494.1 MBL fold metallo-hydrolase -
  IF703_RS02420 (IF703_02405) comGA 457604..458578 (+) 975 WP_000697222.1 competence type IV pilus ATPase ComGA Machinery gene
  IF703_RS02425 (IF703_02410) comGB 458550..459620 (+) 1071 Protein_446 competence type IV pilus assembly protein ComGB -
  IF703_RS02430 (IF703_02415) comGC 459634..459945 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  IF703_RS02435 (IF703_02420) comGD 459923..460369 (+) 447 WP_072357807.1 competence type IV pilus minor pilin ComGD Machinery gene
  IF703_RS02440 (IF703_02425) comGE 460356..460655 (+) 300 WP_000844415.1 hypothetical protein Machinery gene
  IF703_RS02445 (IF703_02430) comGF 460573..461070 (+) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  IF703_RS02450 (IF703_02435) - 461167..461313 (+) 147 WP_001789879.1 hypothetical protein -
  IF703_RS02455 (IF703_02440) - 461303..461827 (+) 525 WP_001015120.1 shikimate kinase -
  IF703_RS02460 (IF703_02445) gcvT 461986..463077 (+) 1092 WP_000093354.1 glycine cleavage system aminomethyltransferase GcvT -
  IF703_RS02465 (IF703_02450) gcvPA 463097..464443 (+) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  IF703_RS02470 (IF703_02455) gcvPB 464436..465908 (+) 1473 WP_000202177.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19179.92 Da        Isoelectric Point: 10.2192

>NTDB_id=488863 IF703_RS02445 WP_001825340.1 460573..461070(+) (comGF) [Staphylococcus aureus strain NAS_AN_068]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=488863 IF703_RS02445 WP_001825340.1 460573..461070(+) (comGF) [Staphylococcus aureus strain NAS_AN_068]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S6DDC2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

96.97

100

0.97