Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   IF707_RS02400 Genome accession   NZ_CP062376
Coordinates   451523..452020 (+) Length   165 a.a.
NCBI ID   WP_001789864.1    Uniprot ID   -
Organism   Staphylococcus aureus strain NAS_AN_099     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 446523..457020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF707_RS02360 (IF707_02360) - 446567..447553 (+) 987 WP_000161314.1 ROK family glucokinase -
  IF707_RS02365 (IF707_02365) - 447553..447882 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  IF707_RS02370 (IF707_02370) - 447879..448502 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  IF707_RS02375 (IF707_02375) comGA 448554..449528 (+) 975 WP_069995863.1 competence type IV pilus ATPase ComGA Machinery gene
  IF707_RS02380 (IF707_02380) comGB 449500..450570 (+) 1071 WP_045176802.1 competence type IV pilus assembly protein ComGB Machinery gene
  IF707_RS02385 (IF707_02385) comGC 450584..450895 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  IF707_RS02390 (IF707_02390) comGD 450873..451319 (+) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  IF707_RS02395 (IF707_02395) comGE 451306..451605 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  IF707_RS02400 (IF707_02400) comGF 451523..452020 (+) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  IF707_RS02405 (IF707_02405) - 452117..452263 (+) 147 WP_001789879.1 hypothetical protein -
  IF707_RS02410 (IF707_02410) - 452253..452777 (+) 525 WP_001015121.1 shikimate kinase -
  IF707_RS02415 (IF707_02415) gcvT 452936..454027 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  IF707_RS02420 (IF707_02420) gcvPA 454047..455393 (+) 1347 WP_045177127.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  IF707_RS02425 (IF707_02425) gcvPB 455386..456858 (+) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19164.88 Da        Isoelectric Point: 10.2192

>NTDB_id=488738 IF707_RS02400 WP_001789864.1 451523..452020(+) (comGF) [Staphylococcus aureus strain NAS_AN_099]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=488738 IF707_RS02400 WP_001789864.1 451523..452020(+) (comGF) [Staphylococcus aureus strain NAS_AN_099]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(67-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

100

100

1
  comGF Staphylococcus aureus N315

96.364

100

0.964