Detailed information NTG0048862

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: RSPO_RS03245
• Genome accession: NC_017574
• Coordinates: 712864..713745 (-)
• Length: 293 a.a.
• NCBI ID: WP_014616067.1
• Uniprot ID: F6FY26
• Organism: Ralstonia solanacearum Po82
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 707864..718745

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RSPO_RS03215 (RSPO_c00675)	argJ	708568..709797 (+)	1230	WP_014616063.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
RSPO_RS03220 (RSPO_c00676)	-	709813..710697 (+)	885	WP_014616064.1	ATP-binding protein	-
RSPO_RS03225 (RSPO_c00677)	-	710675..711136 (+)	462	WP_014616065.1	NUDIX domain-containing protein	-
RSPO_RS03230 (RSPO_c00678)	-	711129..711344 (-)	216	WP_003266032.1	DNA gyrase inhibitor YacG	-
RSPO_RS03235 (RSPO_c00679)	zapD	711361..712119 (-)	759	WP_003266031.1	cell division protein ZapD	-
RSPO_RS03240 (RSPO_c00680)	coaE	712237..712851 (-)	615	WP_014616066.1	dephospho-CoA kinase	-
RSPO_RS03245 (RSPO_c00681)	pilD	712864..713745 (-)	882	WP_014616067.1	prepilin peptidase	Machinery gene
RSPO_RS03250 (RSPO_c00682)	pilG	713748..715013 (-)	1266	WP_014616068.1	type II secretion system F family protein	Machinery gene
RSPO_RS03255 (RSPO_c00683)	pilF	715058..716785 (-)	1728	WP_014616069.1	type IV-A pilus assembly ATPase PilB	Machinery gene
RSPO_RS03260 (RSPO_c00684)	-	717033..718310 (-)	1278	WP_014616070.1	HlyC/CorC family transporter	-

Sequence

Protein

• Length: 293 a.a.
• Molecular weight: 31552.49 Da
• Isoelectric Point: 6.8369

>NTDB_id=48862 RSPO_RS03245 WP_014616067.1 712864..713745(-) (pilD) [Ralstonia solanacearum Po82]
MSGMFAIPTLALLPAWFVIGAAALLGLLVGSFLNVVIHRLPSMIEREEANYIAELRDEPLPHPEPYNLVVPRSACPSCGH
QIKAIENIPVISWLALRGRCSACKTPISWRYPAVELVTSVLTGACLWHFGPTWVAVASAVLLWFLIAGTMIDADTQLLPD
AVTQPLLWLGLLVNLFDMFARLPDAVIGAMAGYLFLWAIYWAYKLLRGREGMGYGDFKLMAALGAWFGWQALPLLILLSS
VVGLVFGAARIARGAESDSPFSFGPFIAGAGVIALLAGPQLVALTGLAPLLAR

Nucleotide

• Length: 882 bp

>NTDB_id=48862 RSPO_RS03245 WP_014616067.1 712864..713745(-) (pilD) [Ralstonia solanacearum Po82]
ATGTCCGGGATGTTCGCCATTCCCACGCTGGCACTGCTGCCCGCGTGGTTCGTGATCGGCGCGGCGGCGCTGCTGGGGCT
GCTGGTCGGCAGCTTCCTCAACGTGGTGATCCACCGCTTGCCGAGCATGATCGAACGCGAGGAGGCCAACTACATCGCCG
AGCTGCGCGACGAGCCACTGCCGCATCCCGAGCCGTACAACCTGGTCGTGCCGCGCTCGGCCTGCCCGTCGTGCGGCCAC
CAGATCAAGGCGATCGAGAACATCCCGGTGATCAGTTGGCTGGCATTGCGCGGCCGCTGCAGCGCCTGCAAGACGCCCAT
CTCCTGGCGCTACCCCGCCGTGGAACTGGTGACCAGCGTACTGACCGGCGCCTGCCTGTGGCATTTCGGACCGACCTGGG
TGGCGGTGGCCTCGGCCGTGTTGCTGTGGTTCCTGATCGCCGGCACGATGATCGACGCCGACACGCAACTGCTGCCCGAT
GCCGTCACCCAGCCGCTGCTGTGGCTCGGCCTGCTGGTGAACCTGTTCGACATGTTCGCGCGGCTGCCGGACGCGGTGAT
CGGCGCGATGGCCGGGTATCTGTTCCTGTGGGCCATCTACTGGGCCTACAAGCTGCTGCGCGGCCGGGAAGGCATGGGCT
ACGGCGACTTCAAGCTGATGGCCGCGCTCGGTGCCTGGTTCGGCTGGCAGGCGCTGCCGTTGCTGATCCTGCTGTCGTCG
GTGGTCGGCCTGGTGTTCGGGGCGGCGCGCATCGCGCGTGGCGCCGAAAGCGACTCACCGTTTTCGTTCGGCCCGTTCAT
CGCGGGCGCCGGCGTCATCGCCCTGCTGGCTGGCCCACAGCTGGTGGCATTGACGGGACTCGCACCGCTGCTGGCGCGGT
AA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (24-129)

• Type IV leader peptidase family (Peptidase_A24) (140-247)

3D structure

• AlphaFold DB: F6FY26

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.585	90.444	0.485
pilD	Vibrio campbellii strain DS40M4	50.549	93.174	0.471
pilD	Neisseria gonorrhoeae MS11	49.627	91.468	0.454
pilD	Acinetobacter baumannii D1279779	46.992	90.785	0.427
pilD	Acinetobacter nosocomialis M2	46.617	90.785	0.423