Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   IF721_RS02415 Genome accession   NZ_CP062362
Coordinates   447154..447651 (+) Length   165 a.a.
NCBI ID   WP_020978197.1    Uniprot ID   -
Organism   Staphylococcus aureus strain NAS_AN_152     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 442154..452651
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF721_RS02375 (IF721_02370) - 442198..443184 (+) 987 WP_000161314.1 ROK family glucokinase -
  IF721_RS02380 (IF721_02375) - 443184..443513 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  IF721_RS02385 (IF721_02380) - 443510..444133 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  IF721_RS02390 (IF721_02385) comGA 444185..445159 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  IF721_RS02395 (IF721_02390) comGB 445131..446201 (+) 1071 WP_000776401.1 competence type IV pilus assembly protein ComGB Machinery gene
  IF721_RS02400 (IF721_02395) comGC 446215..446526 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  IF721_RS02405 (IF721_02400) comGD 446504..446950 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  IF721_RS02410 (IF721_02405) comGE 446937..447236 (+) 300 WP_000844410.1 hypothetical protein Machinery gene
  IF721_RS02415 (IF721_02410) comGF 447154..447651 (+) 498 WP_020978197.1 competence type IV pilus minor pilin ComGF Machinery gene
  IF721_RS02420 (IF721_02415) - 447748..447894 (+) 147 WP_001789879.1 hypothetical protein -
  IF721_RS02425 (IF721_02420) - 447884..448408 (+) 525 WP_001015121.1 shikimate kinase -
  IF721_RS02430 (IF721_02425) gcvT 448567..449658 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  IF721_RS02435 (IF721_02430) gcvPA 449678..451024 (+) 1347 WP_240024439.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  IF721_RS02440 (IF721_02435) gcvPB 451017..452489 (+) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19158.95 Da        Isoelectric Point: 10.1964

>NTDB_id=488488 IF721_RS02415 WP_020978197.1 447154..447651(+) (comGF) [Staphylococcus aureus strain NAS_AN_152]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILQKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=488488 IF721_RS02415 WP_020978197.1 447154..447651(+) (comGF) [Staphylococcus aureus strain NAS_AN_152]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACAGAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.788

100

0.988
  comGF Staphylococcus aureus MW2

96.364

100

0.964