Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   IF721_RS02165 Genome accession   NZ_CP062362
Coordinates   399634..400320 (+) Length   228 a.a.
NCBI ID   WP_001792568.1    Uniprot ID   A0A133PXY3
Organism   Staphylococcus aureus strain NAS_AN_152     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 394634..405320
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF721_RS02130 (IF721_02125) yqeH 395161..396261 (+) 1101 WP_001280138.1 ribosome biogenesis GTPase YqeH -
  IF721_RS02135 (IF721_02130) aroE 396275..397081 (+) 807 WP_000666757.1 shikimate dehydrogenase -
  IF721_RS02140 (IF721_02135) yhbY 397085..397375 (+) 291 WP_000955231.1 ribosome assembly RNA-binding protein YhbY -
  IF721_RS02145 (IF721_02140) nadD 397378..397947 (+) 570 WP_000725162.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  IF721_RS02150 (IF721_02145) yqeK 397937..398521 (+) 585 WP_001019323.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  IF721_RS02155 (IF721_02150) rsfS 398522..398875 (+) 354 WP_001088022.1 ribosome silencing factor -
  IF721_RS02160 (IF721_02155) - 398878..399594 (+) 717 WP_000084829.1 class I SAM-dependent methyltransferase -
  IF721_RS02165 (IF721_02160) comEA 399634..400320 (+) 687 WP_001792568.1 ComEA family DNA-binding protein Machinery gene
  IF721_RS02170 (IF721_02165) - 400412..400873 (+) 462 WP_000439693.1 ComE operon protein 2 -
  IF721_RS02175 (IF721_02170) comEC 400878..403079 (+) 2202 WP_049308125.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  IF721_RS02180 (IF721_02175) holA 403136..404110 (+) 975 WP_001282562.1 DNA polymerase III subunit delta -
  IF721_RS02185 (IF721_02180) rpsT 404155..404406 (-) 252 WP_001274017.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25863.49 Da        Isoelectric Point: 9.3305

>NTDB_id=488480 IF721_RS02165 WP_001792568.1 399634..400320(+) (comEA) [Staphylococcus aureus strain NAS_AN_152]
MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

Nucleotide


Download         Length: 687 bp        

>NTDB_id=488480 IF721_RS02165 WP_001792568.1 399634..400320(+) (comEA) [Staphylococcus aureus strain NAS_AN_152]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTCTAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAAGTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAGGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGAAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTTGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A133PXY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

98.238

99.561

0.978
  comEA Staphylococcus aureus N315

97.357

99.561

0.969