Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   IF736_RS02135 Genome accession   NZ_CP062350
Coordinates   398286..398972 (+) Length   228 a.a.
NCBI ID   WP_072361794.1    Uniprot ID   -
Organism   Staphylococcus aureus strain NAS_AN_250     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 393286..403972
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF736_RS02100 (IF736_02090) yqeH 393813..394913 (+) 1101 WP_001280141.1 ribosome biogenesis GTPase YqeH -
  IF736_RS02105 (IF736_02095) aroE 394927..395733 (+) 807 WP_000666761.1 shikimate dehydrogenase -
  IF736_RS02110 (IF736_02100) yhbY 395737..396027 (+) 291 WP_240018962.1 ribosome assembly RNA-binding protein YhbY -
  IF736_RS02115 (IF736_02105) nadD 396030..396599 (+) 570 WP_106905719.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  IF736_RS02120 (IF736_02110) yqeK 396589..397173 (+) 585 WP_001019326.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  IF736_RS02125 (IF736_02115) rsfS 397174..397527 (+) 354 WP_001088020.1 ribosome silencing factor -
  IF736_RS02130 (IF736_02120) - 397530..398246 (+) 717 WP_000084822.1 class I SAM-dependent methyltransferase -
  IF736_RS02135 (IF736_02125) comEA 398286..398972 (+) 687 WP_072361794.1 ComEA family DNA-binding protein Machinery gene
  IF736_RS02140 (IF736_02130) - 399064..399525 (+) 462 WP_000439693.1 ComE operon protein 2 -
  IF736_RS02145 (IF736_02135) comEC 399530..401731 (+) 2202 WP_025174327.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  IF736_RS02150 (IF736_02140) holA 401788..402762 (+) 975 WP_001282563.1 DNA polymerase III subunit delta -
  IF736_RS02155 (IF736_02145) rpsT 402807..403058 (-) 252 WP_001274017.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25863.49 Da        Isoelectric Point: 9.3305

>NTDB_id=488227 IF736_RS02135 WP_072361794.1 398286..398972(+) (comEA) [Staphylococcus aureus strain NAS_AN_250]
MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

Nucleotide


Download         Length: 687 bp        

>NTDB_id=488227 IF736_RS02135 WP_072361794.1 398286..398972(+) (comEA) [Staphylococcus aureus strain NAS_AN_250]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAAGTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAGGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGAAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTTGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

98.678

99.561

0.982
  comEA Staphylococcus aureus N315

97.797

99.561

0.974