Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   IF741_RS02370 Genome accession   NZ_CP062342
Coordinates   442076..442573 (+) Length   165 a.a.
NCBI ID   WP_029642894.1    Uniprot ID   -
Organism   Staphylococcus aureus strain NAS_AN_270     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 437076..447573
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF741_RS02330 (IF741_02330) - 437120..438106 (+) 987 WP_000161314.1 ROK family glucokinase -
  IF741_RS02335 (IF741_02335) - 438106..438435 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  IF741_RS02340 (IF741_02340) - 438432..439055 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  IF741_RS02345 (IF741_02345) comGA 439107..440081 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  IF741_RS02350 (IF741_02350) comGB 440053..441123 (+) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  IF741_RS02355 (IF741_02355) comGC 441137..441448 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  IF741_RS02360 (IF741_02360) comGD 441426..441872 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  IF741_RS02365 (IF741_02365) comGE 441859..442158 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  IF741_RS02370 (IF741_02370) comGF 442076..442573 (+) 498 WP_029642894.1 competence type IV pilus minor pilin ComGF Machinery gene
  IF741_RS02375 (IF741_02375) - 442670..442816 (+) 147 WP_001789879.1 hypothetical protein -
  IF741_RS02380 (IF741_02380) - 442806..443330 (+) 525 WP_001015119.1 shikimate kinase -
  IF741_RS02385 (IF741_02385) gcvT 443489..444580 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  IF741_RS02390 (IF741_02390) gcvPA 444600..445946 (+) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  IF741_RS02395 (IF741_02395) gcvPB 445939..447411 (+) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19196.92 Da        Isoelectric Point: 10.2192

>NTDB_id=488071 IF741_RS02370 WP_029642894.1 442076..442573(+) (comGF) [Staphylococcus aureus strain NAS_AN_270]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTNVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=488071 IF741_RS02370 WP_029642894.1 442076..442573(+) (comGF) [Staphylococcus aureus strain NAS_AN_270]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAAATGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(70-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

97.576

100

0.976
  comGF Staphylococcus aureus MW2

97.576

100

0.976