Detailed information    

insolico Bioinformatically predicted

Overview

Name   hexB   Type   Machinery gene
Locus tag   KEF49_RS08200 Genome accession   NZ_CP073635
Coordinates   1636991..1638868 (+) Length   625 a.a.
NCBI ID   WP_115997262.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain Ba13     
Function   DNA mismatch repair (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1631991..1643868
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KEF49_RS08185 (KEF49_08185) ymcA 1633045..1633476 (+) 432 WP_065981489.1 RicAFT regulatory complex protein RicA family protein Regulator
  KEF49_RS08190 (KEF49_08190) cotE 1633729..1634274 (+) 546 WP_065981490.1 outer spore coat protein CotE -
  KEF49_RS08195 (KEF49_08195) hexA 1634390..1636975 (+) 2586 WP_115997807.1 DNA mismatch repair protein MutS Machinery gene
  KEF49_RS08200 (KEF49_08200) hexB 1636991..1638868 (+) 1878 WP_115997262.1 DNA mismatch repair endonuclease MutL Machinery gene
  KEF49_RS08205 (KEF49_08205) - 1638920..1640035 (-) 1116 WP_212101404.1 site-specific integrase -
  KEF49_RS08210 (KEF49_08210) - 1640151..1640976 (+) 826 Protein_1578 DNA adenine methylase -
  KEF49_RS08215 (KEF49_08215) - 1641092..1641610 (-) 519 WP_065981493.1 hypothetical protein -
  KEF49_RS08220 (KEF49_08220) - 1641748..1642182 (+) 435 WP_065981494.1 hypothetical protein -
  KEF49_RS08225 (KEF49_08225) - 1642327..1642704 (+) 378 WP_065981495.1 hypothetical protein -
  KEF49_RS08230 (KEF49_08230) - 1642736..1643323 (+) 588 WP_044051884.1 VanZ family protein -

Sequence

Protein


Download         Length: 625 a.a.        Molecular weight: 70280.47 Da        Isoelectric Point: 6.0947

>NTDB_id=487628 KEF49_RS08200 WP_115997262.1 1636991..1638868(+) (hexB) [Bacillus amyloliquefaciens strain Ba13]
MAKVIQLSDELSNKIAAGEVVERPASVVKELVENAIDANSTVIEIDIEEAGLSSIRVLDNGEGMETEDCKRAFRRHATSK
IKDENDLFRVRTLGFRGEALPSIASVSHLEIKTSIGEGAGTHLILQGGNMISEQRSSSRKGTEIIVTNLFFNTPARLKYM
KTVHTELGNITDVVNRIALAHPEVSIRLRHQGKNLLQTNGNGDVRHVLAAIYGTAVAKKMIPLHVSSLDFEVKGYIALPE
ITRASRNYMSSVINGRYIKNFPLVKAVHEGYHTLLPIGRHPITFIEITMDPILVDVNVHPSKLEVRLSKETELHELIRDG
IKEVFQKQRLIPSAQLPKKSAPAPVKNEQQFMTFGESAPERKLPEKTPEPSYSPMKLSSVVKEPAPITEEAFHPNETDHE
VPPETSVPETAAPVFDIPAPEEEAVSEEIQEERVPVMYPIGQMHGTYILAQNENGLYIIDQHAAQERIKYEYFREKVGQV
EPEVQDMIVPLTFHYAANEALIIEQHQRELESVGVFLEAFGTNSYIVRCHPAWFPKGEEAELIEEIIQQVLDAKQIDIKK
LREEAAIMMSCKGSIKANRHLRNDEIKALLDDLRRTADPFTCPHGRPIIIHHSTYEMEKMFKRVM

Nucleotide


Download         Length: 1878 bp        

>NTDB_id=487628 KEF49_RS08200 WP_115997262.1 1636991..1638868(+) (hexB) [Bacillus amyloliquefaciens strain Ba13]
TTGGCAAAAGTCATCCAGCTGTCAGATGAGCTTTCAAATAAAATAGCGGCCGGTGAGGTTGTGGAACGGCCTGCCTCTGT
CGTTAAAGAATTGGTGGAAAACGCTATCGACGCAAACAGCACAGTCATTGAAATCGATATAGAGGAAGCGGGGCTTTCGT
CGATTCGGGTGCTCGACAACGGCGAAGGTATGGAAACCGAGGATTGCAAGCGGGCTTTCCGCCGCCACGCGACCAGTAAA
ATCAAAGATGAAAATGACCTGTTCCGGGTGAGAACACTCGGTTTCCGCGGAGAAGCTTTGCCAAGTATCGCGTCTGTTTC
TCATCTGGAAATCAAAACGAGCATCGGCGAGGGAGCGGGAACTCATCTCATTCTTCAGGGCGGGAATATGATTTCTGAAC
AAAGATCTTCCAGCAGAAAAGGGACGGAGATTATCGTCACTAACCTGTTTTTCAATACACCCGCCAGATTAAAATATATG
AAAACCGTGCATACGGAGCTCGGCAATATTACGGATGTCGTCAACCGGATCGCCCTTGCGCATCCGGAAGTATCGATCCG
CCTCCGCCATCAGGGGAAAAACCTGCTGCAGACAAACGGAAACGGTGACGTGCGCCATGTGCTGGCCGCAATTTACGGGA
CGGCGGTTGCGAAAAAAATGATACCGCTCCATGTCAGCTCACTTGATTTTGAAGTCAAGGGCTACATTGCGCTGCCGGAA
ATCACGCGTGCATCCAGAAACTACATGTCATCTGTCATTAACGGAAGATACATTAAGAATTTCCCGCTTGTAAAAGCTGT
GCATGAAGGCTATCACACACTTCTGCCGATCGGCCGCCATCCGATTACGTTTATCGAAATTACGATGGATCCGATTTTGG
TCGATGTCAATGTCCATCCGTCGAAGCTTGAAGTCCGCCTCAGCAAAGAGACGGAACTGCATGAGCTGATCCGTGACGGC
ATAAAGGAAGTCTTTCAAAAGCAGCGGCTTATTCCGAGCGCCCAGCTTCCGAAAAAATCAGCGCCGGCACCGGTCAAAAA
TGAACAGCAATTTATGACGTTCGGTGAAAGTGCGCCTGAGCGGAAACTGCCTGAAAAAACGCCTGAGCCGTCTTACTCGC
CAATGAAGCTGAGTTCGGTCGTAAAAGAGCCGGCGCCGATAACAGAAGAAGCGTTTCACCCAAATGAAACGGATCATGAA
GTGCCGCCGGAGACGAGCGTGCCGGAAACCGCTGCGCCTGTTTTCGATATTCCGGCGCCGGAAGAGGAAGCTGTCTCCGA
AGAAATACAGGAAGAAAGAGTTCCGGTCATGTATCCGATCGGCCAAATGCACGGGACATATATTTTAGCCCAAAACGAAA
ACGGCCTGTATATCATTGACCAGCATGCGGCCCAAGAGCGGATTAAATACGAATATTTCCGGGAAAAAGTCGGGCAGGTT
GAGCCTGAAGTGCAGGATATGATCGTGCCGCTCACCTTTCACTATGCGGCAAATGAAGCGCTTATCATTGAACAGCATCA
GCGTGAGCTTGAAAGTGTAGGGGTATTTTTAGAAGCATTCGGCACTAACAGCTATATCGTCCGCTGCCATCCGGCCTGGT
TTCCAAAAGGGGAAGAAGCTGAACTGATTGAAGAAATTATTCAGCAGGTGCTTGACGCCAAACAGATCGACATTAAAAAA
CTTCGGGAGGAAGCCGCCATAATGATGAGCTGCAAAGGCTCGATCAAAGCGAACCGTCATTTGCGCAACGACGAAATCAA
AGCGCTCTTGGACGATCTGCGCCGAACGGCAGACCCGTTCACATGCCCGCACGGCCGGCCGATCATTATTCACCATAGTA
CGTATGAGATGGAAAAGATGTTTAAACGTGTGATGTAG

Domains

Predicted by InterProScan.

MutL C terminal dimerisation domain (MutL_C)

(440-581)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c_3)

(25-85)

DNA mismatch repair protein, C-terminal domain (DNA_mis_repair)

(209-325)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  hexB Streptococcus pneumoniae R6

46.368

100

0.48