Detailed information NTG0487627

Bioinformatically predicted

Overview

• Name: hexA
• Type: Machinery gene
• Locus tag: KEF49_RS08195
• Genome accession: NZ_CP073635
• Coordinates: 1634390..1636975 (+)
• Length: 861 a.a.
• NCBI ID: WP_115997807.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain Ba13
• Function: DNA mismatch repair (predicted from homology) Homologous recombination

Genomic Context

Location: 1629390..1641975

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KEF49_RS08175 (KEF49_08175)	-	1630195..1631370 (+)	1176	WP_065981487.1	glycine C-acetyltransferase	-
KEF49_RS08180 (KEF49_08180)	miaB	1631513..1633042 (+)	1530	WP_065981488.1	tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB	-
KEF49_RS08185 (KEF49_08185)	ymcA	1633045..1633476 (+)	432	WP_065981489.1	RicAFT regulatory complex protein RicA family protein	Regulator
KEF49_RS08190 (KEF49_08190)	cotE	1633729..1634274 (+)	546	WP_065981490.1	outer spore coat protein CotE	-
KEF49_RS08195 (KEF49_08195)	hexA	1634390..1636975 (+)	2586	WP_115997807.1	DNA mismatch repair protein MutS	Machinery gene
KEF49_RS08200 (KEF49_08200)	hexB	1636991..1638868 (+)	1878	WP_115997262.1	DNA mismatch repair endonuclease MutL	Machinery gene
KEF49_RS08205 (KEF49_08205)	-	1638920..1640035 (-)	1116	WP_212101404.1	site-specific integrase	-
KEF49_RS08210 (KEF49_08210)	-	1640151..1640976 (+)	826	Protein_1578	DNA adenine methylase	-
KEF49_RS08215 (KEF49_08215)	-	1641092..1641610 (-)	519	WP_065981493.1	hypothetical protein	-

Sequence

Protein

• Length: 861 a.a.
• Molecular weight: 97524.93 Da
• Isoelectric Point: 5.5611

>NTDB_id=487627 KEF49_RS08195 WP_115997807.1 1634390..1636975(+) (hexA) [Bacillus amyloliquefaciens strain Ba13]
MASYTPMIQQYLKIKAEYQDAFLFFRLGDFYEMFFEDAKKASQELEITLTSRDGGSADKIPMCGVPYHSAAAYIEQLIKK
GYKVAICEQTEDPKAAKGVVKREVVQLITPGTVMDGKGIHESENNFIASVSAFPGGYGLALSDLTTGENLAVFIERIEDV
MSEIYSVGAKEIVVSSKFNENTAAQLKERCGATISIEDGKMTERIEIAKHLPGEELTETFMRLYTYLQKTQKRSLDHLQP
VQVYELEEAMKIDLYSKRNLELTETIRSKSKKGSLLWLLDETKTAMGGRLLKQWIDRPLIRASQIEERQEMVETLINHLF
EREDLRERLKEVYDLERLAGRVAFGNVNARDLIQLKESLKQVPSIKELVGSLNHKKAKERAGLIDPCGDLLDLLEDALHE
NPPLSLKEGNLIKDGYHQKLDEYRDASKNGKDWIARLEQQERAYTGIRSLKVGFNKVFGYYIEVTKANLHLLEDGRYERK
QTLTNAERYITPELKEKEALILEAENNICELEYELFAGLREKVKQFIPRLQRLAKQMSELDALQCFATISENRHYTKPVF
SDNEVKVIEGRHPVVEKVMDSQEYVPNNCLMGESREMLLITGPNMSGKSTYMRQIALLSIMAQIGCFVPAKEAVLPIFDQ
IFTRIGAADDLISGQSTFMVEMLEAKNAIVNATKDSLILFDEIGRGTSTYDGMALAQAIIEYVHDHIGAKTLFSTHYHEL
TVLEDKLPQLKNVHVRAEEYNGKVVFLHQIKEGAADKSYGIHVAQLAELPGDLISRAQEILKQLEQSGDKPELPAAPEKK
PAVKEEPAQLSFFADGEKEQKAPAVSHKEKQVLEAFKSINILDMTPLEAMNEMYKLQKKLK

Nucleotide

• Length: 2586 bp

>NTDB_id=487627 KEF49_RS08195 WP_115997807.1 1634390..1636975(+) (hexA) [Bacillus amyloliquefaciens strain Ba13]
ATGGCTAGTTACACGCCTATGATACAGCAATACTTGAAGATAAAGGCAGAATACCAAGATGCCTTTTTATTTTTTCGGCT
TGGTGACTTTTATGAAATGTTTTTTGAAGATGCAAAAAAAGCGTCACAAGAGCTTGAAATTACACTGACAAGCAGGGACG
GAGGATCGGCTGATAAAATTCCGATGTGCGGCGTGCCGTATCATTCCGCGGCGGCTTATATTGAACAGCTGATTAAAAAG
GGCTATAAAGTGGCGATCTGTGAACAGACCGAAGATCCGAAGGCGGCCAAAGGCGTTGTCAAAAGAGAAGTCGTCCAGCT
GATCACACCGGGAACCGTGATGGACGGAAAAGGCATTCATGAGTCGGAAAACAATTTTATCGCTTCCGTTTCCGCATTTC
CTGGCGGATACGGCCTCGCTTTATCAGACTTAACGACTGGAGAAAACCTGGCCGTATTTATTGAACGGATTGAAGATGTC
ATGTCAGAAATTTATTCAGTCGGCGCAAAGGAAATTGTCGTATCAAGCAAGTTTAATGAAAATACAGCGGCCCAGCTGAA
AGAACGGTGCGGCGCAACGATTTCCATCGAAGACGGGAAAATGACAGAACGGATTGAAATCGCAAAGCACTTGCCTGGCG
AAGAGCTGACAGAGACGTTTATGCGTTTATATACGTATTTGCAGAAAACGCAAAAACGGAGCCTGGATCACCTGCAGCCG
GTTCAAGTATATGAATTGGAAGAAGCGATGAAAATTGATCTGTATTCAAAACGGAATCTTGAACTGACGGAAACGATCCG
TTCGAAAAGCAAAAAAGGATCACTGCTCTGGCTTTTGGACGAGACGAAAACAGCAATGGGCGGCAGGCTCCTCAAACAAT
GGATTGACCGCCCGCTGATCAGGGCATCCCAAATCGAAGAACGTCAGGAAATGGTCGAGACGCTGATCAATCATCTGTTT
GAGCGTGAAGATTTGCGCGAACGCTTGAAAGAAGTGTATGATTTGGAGCGTCTTGCGGGCCGGGTCGCATTCGGAAACGT
CAATGCAAGGGATTTAATCCAGCTGAAAGAATCGTTAAAACAGGTGCCGAGCATTAAAGAGCTTGTAGGGTCTCTTAACC
ATAAAAAAGCAAAAGAACGCGCCGGCTTGATTGACCCGTGCGGAGATCTGCTTGACCTGTTAGAAGATGCGCTGCATGAA
AACCCGCCGTTATCTTTAAAGGAAGGCAACTTAATAAAAGACGGCTATCATCAGAAACTCGATGAGTACCGTGACGCCAG
CAAAAACGGCAAAGACTGGATTGCCAGACTGGAGCAGCAGGAGCGTGCGTATACGGGCATCCGTTCATTAAAGGTTGGCT
TTAATAAAGTGTTCGGCTATTATATTGAAGTCACAAAAGCAAACCTTCATCTCCTTGAGGACGGGCGGTATGAGCGGAAG
CAGACGCTCACAAACGCTGAACGGTACATTACGCCGGAATTAAAGGAAAAAGAAGCGCTTATTTTAGAAGCGGAAAACAA
CATTTGCGAGCTGGAATATGAGCTTTTTGCCGGACTGCGTGAAAAGGTTAAGCAATTTATTCCGCGCCTGCAGCGTCTAG
CCAAGCAAATGAGTGAGCTGGACGCACTGCAATGCTTTGCAACGATCAGTGAAAACAGGCATTACACAAAGCCCGTCTTT
TCCGATAATGAGGTAAAAGTCATTGAAGGCAGACATCCTGTCGTTGAAAAGGTGATGGACAGTCAAGAATACGTGCCGAA
CAATTGCCTGATGGGCGAATCACGGGAAATGCTCCTGATTACGGGTCCGAATATGTCAGGTAAAAGCACGTACATGAGAC
AGATCGCACTTTTATCAATCATGGCCCAGATCGGCTGTTTTGTGCCCGCAAAAGAAGCGGTTCTGCCGATTTTTGATCAA
ATCTTTACGAGAATCGGGGCTGCGGACGATTTGATTTCCGGACAAAGCACCTTTATGGTGGAAATGCTGGAAGCGAAAAA
CGCGATTGTAAACGCCACAAAAGACAGCCTGATCCTGTTCGATGAAATCGGACGCGGGACATCAACGTATGACGGAATGG
CGCTGGCACAGGCAATCATTGAATATGTCCATGATCATATCGGCGCTAAAACACTGTTCAGCACGCACTATCATGAACTG
ACGGTGCTTGAAGATAAGCTTCCTCAGCTGAAAAACGTTCATGTGCGCGCCGAGGAATATAACGGAAAGGTCGTGTTTCT
CCATCAAATCAAAGAAGGAGCCGCCGATAAAAGTTACGGCATTCATGTCGCCCAGCTTGCTGAGCTCCCGGGTGATCTGA
TCAGCCGCGCGCAGGAGATTCTGAAACAGCTTGAGCAGTCAGGAGATAAACCGGAGCTTCCGGCGGCACCTGAGAAAAAG
CCCGCCGTCAAGGAAGAACCGGCACAGCTGTCCTTTTTTGCAGACGGTGAAAAAGAACAGAAAGCACCGGCTGTTTCTCA
TAAAGAAAAACAAGTGCTTGAAGCATTTAAGTCGATCAATATTCTTGATATGACGCCTCTTGAGGCGATGAATGAAATGT
ACAAGCTGCAAAAAAAATTAAAATAG

Domains

Predicted by InterProScan.

• MutS family domain IV (MutS_IV) (415-506)

• MutS domain I (MutS_I) (5-116)

• MutS domain III (MutS_III) (258-547)

• MutS domain II (MutS_II) (125-239)

• MutS domain V (MutS_V) (598-785)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
hexA	Streptococcus pneumoniae R6	48.308	99.535	0.481
mutS	Pseudomonas stutzeri strain ATCC 17587	38.629	100	0.393