Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   IF776_RS02420 Genome accession   NZ_CP062314
Coordinates   455278..455775 (+) Length   165 a.a.
NCBI ID   WP_001789864.1    Uniprot ID   -
Organism   Staphylococcus aureus strain NAS_OP_133     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 450278..460775
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF776_RS02380 (IF776_02390) - 450322..451308 (+) 987 WP_000161314.1 ROK family glucokinase -
  IF776_RS02385 (IF776_02395) - 451308..451637 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  IF776_RS02390 (IF776_02400) - 451634..452257 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  IF776_RS02395 (IF776_02405) comGA 452309..453283 (+) 975 WP_239794875.1 competence type IV pilus ATPase ComGA Machinery gene
  IF776_RS02400 (IF776_02410) comGB 453255..454325 (+) 1071 WP_000775708.1 competence type IV pilus assembly protein ComGB Machinery gene
  IF776_RS02405 (IF776_02415) comGC 454339..454650 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  IF776_RS02410 (IF776_02420) comGD 454628..455074 (+) 447 WP_001791635.1 competence type IV pilus minor pilin ComGD Machinery gene
  IF776_RS02415 (IF776_02425) comGE 455061..455360 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  IF776_RS02420 (IF776_02430) comGF 455278..455775 (+) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  IF776_RS02425 (IF776_02435) - 455872..456018 (+) 147 WP_001789879.1 hypothetical protein -
  IF776_RS02430 (IF776_02440) - 456008..456532 (+) 525 WP_001015117.1 shikimate kinase -
  IF776_RS02435 (IF776_02445) gcvT 456691..457782 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  IF776_RS02440 (IF776_02450) gcvPA 457802..459148 (+) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  IF776_RS02445 (IF776_02455) gcvPB 459141..460613 (+) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19164.88 Da        Isoelectric Point: 10.2192

>NTDB_id=487576 IF776_RS02420 WP_001789864.1 455278..455775(+) (comGF) [Staphylococcus aureus strain NAS_OP_133]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=487576 IF776_RS02420 WP_001789864.1 455278..455775(+) (comGF) [Staphylococcus aureus strain NAS_OP_133]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(67-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

100

100

1
  comGF Staphylococcus aureus N315

96.364

100

0.964