Detailed information NTG0486924

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: IGW72_RS16305
• Genome accession: NZ_CP062219
• Coordinates: 3547083..3547955 (+)
• Length: 290 a.a.
• NCBI ID: WP_023084740.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain JT86
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3542083..3552955

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IGW72_RS16290	-	3543466..3543930 (-)	465	WP_023129290.1	pilin	-
IGW72_RS16295	pilB	3544161..3545861 (+)	1701	WP_003107297.1	type IV-A pilus assembly ATPase PilB	Machinery gene
IGW72_RS16300	pilC	3545865..3547082 (+)	1218	WP_003161763.1	type II secretion system F family protein	Machinery gene
IGW72_RS16305	pilD	3547083..3547955 (+)	873	WP_023084740.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
IGW72_RS16310	coaE	3547952..3548563 (+)	612	WP_003112838.1	dephospho-CoA kinase	-
IGW72_RS16315	yacG	3548560..3548760 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
IGW72_RS16320	-	3548797..3549006 (-)	210	WP_003094660.1	hypothetical protein	-
IGW72_RS16325	-	3549112..3549801 (-)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
IGW72_RS16330	-	3549798..3550268 (-)	471	WP_003094664.1	hypothetical protein	-
IGW72_RS16335	-	3550265..3550690 (-)	426	WP_009875877.1	GNAT family N-acetyltransferase	-
IGW72_RS16340	-	3550823..3551452 (+)	630	WP_003094668.1	DUF1780 domain-containing protein	-
IGW72_RS16345	-	3551449..3551898 (+)	450	WP_003094670.1	MOSC domain-containing protein	-
IGW72_RS16350	-	3551924..3552094 (+)	171	WP_003094672.1	DUF3094 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31843.88 Da
• Isoelectric Point: 7.7068

>NTDB_id=486924 IGW72_RS16305 WP_023084740.1 3547083..3547955(+) (pilD) [Pseudomonas aeruginosa strain JT86]
MTLLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=486924 IGW72_RS16305 WP_023084740.1 3547083..3547955(+) (pilD) [Pseudomonas aeruginosa strain JT86]
ATGACCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTTGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCCCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGCCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCTTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.714	96.552	0.538
pilD	Vibrio campbellii strain DS40M4	53.004	97.586	0.517
pilD	Acinetobacter nosocomialis M2	50.53	97.586	0.493
pilD	Acinetobacter baumannii D1279779	50.53	97.586	0.493
pilD	Neisseria gonorrhoeae MS11	49.281	95.862	0.472