Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   KCG54_RS08885 Genome accession   NZ_CP073115
Coordinates   1842217..1842675 (+) Length   152 a.a.
NCBI ID   WP_254323970.1    Uniprot ID   -
Organism   Neisseria subflava strain TT0077     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1837217..1847675
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCG54_RS08870 (KCG54_08905) - 1837963..1838259 (+) 297 WP_003680870.1 helix-turn-helix transcriptional regulator -
  KCG54_RS08875 (KCG54_08910) glnD 1838279..1840837 (+) 2559 WP_254323968.1 [protein-PII] uridylyltransferase -
  KCG54_RS08880 (KCG54_08915) pilE 1841629..1842102 (+) 474 WP_254323969.1 pilin Machinery gene
  KCG54_RS08885 (KCG54_08920) pilE 1842217..1842675 (+) 459 WP_254323970.1 pilin Machinery gene
  KCG54_RS08890 (KCG54_08925) - 1843101..1844627 (-) 1527 WP_254323971.1 DHA2 family efflux MFS transporter permease subunit -
  KCG54_RS08895 (KCG54_08930) - 1844729..1845919 (-) 1191 WP_070823422.1 efflux RND transporter periplasmic adaptor subunit -
  KCG54_RS08900 (KCG54_08935) - 1846140..1847546 (-) 1407 WP_070823421.1 efflux transporter outer membrane subunit -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 16280.94 Da        Isoelectric Point: 8.4440

>NTDB_id=486874 KCG54_RS08885 WP_254323970.1 1842217..1842675(+) (pilE) [Neisseria subflava strain TT0077]
MKKLRNAQTGFSLIELMIVIGIIGILTIAALPAYQNYVARAQVAEGIMLAGSMKSYVEEAYADKGSLPLDLNIKQLASDD
NKVGNYVKIVRIEDNGTIVAAIGNQAAVPVKNTEIHLIPSFAKTGNTKGTTVWSCGGTMDTYFLPVSCKQSL

Nucleotide


Download         Length: 459 bp        

>NTDB_id=486874 KCG54_RS08885 WP_254323970.1 1842217..1842675(+) (pilE) [Neisseria subflava strain TT0077]
ATGAAAAAACTACGCAACGCCCAAACCGGCTTTAGCCTGATTGAGTTGATGATTGTTATCGGCATCATCGGCATTTTGAC
AATAGCGGCTCTTCCTGCATACCAAAACTATGTTGCCAGAGCACAAGTAGCAGAAGGCATTATGCTGGCAGGAAGTATGA
AAAGCTACGTCGAAGAAGCCTATGCGGATAAAGGCAGCTTGCCGCTTGACCTCAATATCAAACAACTCGCTTCAGACGAC
AATAAAGTCGGCAACTATGTAAAGATTGTCCGTATTGAAGATAACGGCACTATTGTTGCCGCCATCGGTAATCAAGCCGC
TGTACCTGTGAAGAATACCGAAATTCACCTAATACCAAGCTTTGCGAAAACAGGTAATACCAAAGGCACAACAGTTTGGT
CTTGCGGCGGCACCATGGATACATACTTCCTTCCCGTATCCTGCAAACAAAGCTTGTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(39-149)

Prokaryotic N-terminal methylation motif (N_methyl)

(5-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

39.13

100

0.414
  pilE Neisseria gonorrhoeae MS11

37.654

100

0.401