Detailed information NTG0486552

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KCG38_RS02195
• Genome accession: NZ_CP073080
• Coordinates: 470698..471846 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain NDTH9845
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 465698..476846

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KCG38_RS02170 (KCG38_02170)	-	466849..467145 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
KCG38_RS02175 (KCG38_02175)	-	467142..467735 (-)	594	WP_003084544.1	YggT family protein	-
KCG38_RS02180 (KCG38_02180)	proC	467746..468567 (-)	822	WP_031757004.1	pyrroline-5-carboxylate reductase	-
KCG38_RS02185 (KCG38_02185)	-	468579..469271 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
KCG38_RS02190 (KCG38_02190)	pilT	469486..470520 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
KCG38_RS02195 (KCG38_02195)	pilU	470698..471846 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
KCG38_RS02200 (KCG38_02200)	czcD	471854..472753 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
KCG38_RS02205 (KCG38_02205)	-	472908..473312 (+)	405	WP_003084560.1	NINE protein	-
KCG38_RS02210 (KCG38_02210)	-	473555..474928 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
KCG38_RS02215 (KCG38_02215)	-	474925..476109 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=486552 KCG38_RS02195 WP_003100959.1 470698..471846(+) (pilU) [Pseudomonas aeruginosa strain NDTH9845]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=486552 KCG38_RS02195 WP_003100959.1 470698..471846(+) (pilU) [Pseudomonas aeruginosa strain NDTH9845]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382