Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   IF132_RS12675 Genome accession   NZ_CP061845
Coordinates   1516502..1517521 (-) Length   339 a.a.
NCBI ID   WP_039423006.1    Uniprot ID   A0A099LSE7
Organism   Vibrio navarrensis strain 2540-90     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1511502..1522521
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF132_RS12650 (IF132_13690) aroK 1512312..1512830 (-) 519 WP_039423021.1 shikimate kinase AroK -
  IF132_RS12655 (IF132_13695) pilQ 1513040..1514827 (-) 1788 WP_039423018.1 type IV pilus secretin PilQ Machinery gene
  IF132_RS12660 (IF132_13700) pilP 1514846..1515358 (-) 513 WP_039423015.1 pilus assembly protein PilP Machinery gene
  IF132_RS12665 (IF132_13705) pilO 1515348..1515938 (-) 591 WP_039423012.1 type 4a pilus biogenesis protein PilO Machinery gene
  IF132_RS12670 (IF132_13710) pilN 1515931..1516518 (-) 588 WP_039423009.1 PilN domain-containing protein Machinery gene
  IF132_RS12675 (IF132_13715) pilM 1516502..1517521 (-) 1020 WP_039423006.1 type IV pilus biogenesis protein PilM Machinery gene
  IF132_RS12680 (IF132_13720) - 1517660..1520176 (+) 2517 WP_039423004.1 penicillin-binding protein 1A -
  IF132_RS12685 (IF132_13725) oxyR 1520253..1521146 (-) 894 WP_039423002.1 DNA-binding transcriptional regulator OxyR -
  IF132_RS12690 (IF132_13730) - 1521317..1521574 (+) 258 WP_039422999.1 DUF3624 domain-containing protein -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 38171.94 Da        Isoelectric Point: 7.3958

>NTDB_id=485038 IF132_RS12675 WP_039423006.1 1516502..1517521(-) (pilM) [Vibrio navarrensis strain 2540-90]
MGKPLVTGIDIGHRSLKAVILKPAGNSYALLGYKEIVFERAIVAENHTLNHQEIVNTLKVLNKELPRFRRQVALAVPDSA
VISKQLQIEPIEDEKECEIAILQAFSHQSPFPVDELNIDYVRLHEPAQSKSASQSVQIFATKKEVVESRVRAAQAVRLKP
VLVDMHAHCLFNLRDLAAQRYPDKSAFCLLDVGAQTTSLIIHQREKTHFIKEFAYGVEHLNAAIGDEFSAHDAQEKTERF
IVEMVDRVARQLQLFQSINSQDKPQGLWLCGEGAATPLLLEALSRRLSLECELLNPLGLFEMKVARKKRRSVDWHHFSVA
AGLAIGGIQWLENKHEAHH

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=485038 IF132_RS12675 WP_039423006.1 1516502..1517521(-) (pilM) [Vibrio navarrensis strain 2540-90]
ATGGGTAAACCCTTAGTTACCGGGATTGATATTGGTCATCGCAGCCTCAAGGCTGTCATTCTGAAACCCGCAGGAAATAG
CTATGCCTTGCTGGGATATAAAGAAATTGTATTTGAGCGCGCTATTGTCGCCGAAAACCACACTCTGAATCATCAGGAAA
TTGTCAATACGCTTAAAGTCCTGAATAAAGAGTTGCCGCGTTTCCGTCGTCAGGTGGCGCTGGCCGTGCCGGATAGTGCC
GTGATCAGCAAGCAATTGCAAATTGAACCGATAGAAGATGAGAAAGAGTGTGAAATCGCCATTTTGCAGGCTTTTTCCCA
TCAATCGCCGTTTCCGGTCGATGAGCTGAATATTGATTATGTGCGTTTGCATGAACCCGCGCAGAGCAAAAGCGCCAGTC
AGAGCGTGCAGATCTTTGCCACTAAGAAAGAGGTAGTCGAGAGCCGGGTGCGCGCCGCGCAGGCGGTGAGGTTGAAACCT
GTGTTGGTGGACATGCACGCCCACTGCTTGTTTAACCTGCGAGATCTGGCGGCGCAGCGCTACCCAGACAAAAGTGCTTT
TTGCCTGTTGGATGTGGGCGCGCAAACCACCTCACTGATAATTCATCAGCGCGAGAAAACGCACTTTATCAAAGAGTTCG
CGTATGGAGTGGAGCACTTGAACGCCGCTATTGGCGATGAGTTTAGCGCCCACGATGCGCAGGAGAAGACCGAGCGATTT
ATTGTGGAGATGGTGGATCGCGTCGCGCGACAGTTGCAACTCTTTCAGTCCATCAATAGTCAAGACAAGCCACAAGGGCT
TTGGTTGTGTGGCGAGGGAGCGGCGACGCCCCTGTTGCTTGAGGCGCTCTCTCGTCGTTTGTCGTTGGAGTGCGAACTGC
TCAATCCGCTCGGTCTGTTTGAGATGAAAGTGGCACGCAAAAAACGTCGCAGTGTTGACTGGCACCACTTTTCGGTCGCC
GCTGGGTTAGCCATCGGTGGGATTCAATGGTTGGAGAACAAACATGAAGCACACCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-325)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A099LSE7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

54.867

100

0.549
  pilM Vibrio cholerae strain A1552

47.536

100

0.484