Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   IF130_RS03460 Genome accession   NZ_CP061840
Coordinates   732526..732975 (-) Length   149 a.a.
NCBI ID   WP_010946364.1    Uniprot ID   Q5ZXV4
Organism   Legionella pneumophila subsp. pneumophila strain AW-13-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 727526..737975
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF130_RS03440 (IF130_03440) - 727801..728193 (-) 393 WP_010946360.1 hypothetical protein -
  IF130_RS03445 (IF130_03445) - 728330..728707 (+) 378 WP_010946361.1 hypothetical protein -
  IF130_RS03450 (IF130_03450) - 728908..730215 (+) 1308 WP_011213222.1 tetratricopeptide repeat protein -
  IF130_RS03455 (IF130_03455) comEC 730241..732448 (-) 2208 WP_010946363.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  IF130_RS03460 (IF130_03460) pilE 732526..732975 (-) 450 WP_010946364.1 type IV pilin protein Machinery gene
  IF130_RS03465 (IF130_03465) - 732987..736496 (-) 3510 WP_011213223.1 pilus assembly protein -
  IF130_RS03470 (IF130_03470) - 736509..737021 (-) 513 WP_010946366.1 pilus assembly protein -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16296.57 Da        Isoelectric Point: 7.6922

>NTDB_id=484959 IF130_RS03460 WP_010946364.1 732526..732975(-) (pilE) [Legionella pneumophila subsp. pneumophila strain AW-13-4]
MLSHVHFMKNSRMKQSAFTLVEVLISMVIMGILVSIAYPSYLQYIQKSRRADAHATLTQDQIILERCYSQNFSYAAACGA
LPAFPQTTPNGYYTINISNLTATTYTLTATPVGTQAKDTECASMSINQANVKTAVDSSANAQPECWNPG

Nucleotide


Download         Length: 450 bp        

>NTDB_id=484959 IF130_RS03460 WP_010946364.1 732526..732975(-) (pilE) [Legionella pneumophila subsp. pneumophila strain AW-13-4]
ATGCTCAGTCATGTACATTTTATGAAGAATAGCCGTATGAAACAATCCGCGTTTACCCTGGTTGAAGTTCTGATCAGCAT
GGTCATTATGGGCATTCTGGTTTCAATTGCCTATCCATCCTATTTACAATATATCCAAAAATCCCGTCGTGCCGATGCTC
ACGCCACATTGACACAAGATCAAATTATTTTAGAACGCTGTTATTCACAGAATTTTTCTTATGCTGCGGCGTGTGGCGCC
TTACCAGCATTTCCTCAAACAACGCCGAACGGGTACTATACTATCAATATTTCAAACCTGACAGCCACAACGTATACCTT
AACTGCAACCCCTGTTGGAACTCAAGCCAAAGATACCGAGTGTGCCAGCATGTCAATTAACCAGGCCAATGTAAAAACAG
CAGTAGATTCCTCCGCTAATGCGCAACCAGAATGCTGGAATCCCGGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(12-38)

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(39-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5ZXV4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Legionella pneumophila str. Paris

100

100

1
  pilE Legionella pneumophila strain ERS1305867

100

100

1