NTDB

Overview

Name	pilD	Type	Machinery gene
Locus tag	J8F85_RS03865	Genome accession	NZ_CP072566
Coordinates	811473..812282 (+)	Length	269 a.a.
NCBI ID	WP_001547020.1	Uniprot ID	-
Organism	Escherichia coli strain WG5::TT12A
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 806473..817282

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J8F85_RS03860 (J8F85_03815)	sslE	806707..811275 (+)	4569	WP_001547021.1	lipoprotein metalloprotease SslE	-
J8F85_RS03865 (J8F85_03820)	pilD	811473..812282 (+)	810	WP_001547020.1	prepilin peptidase PppA	Machinery gene
J8F85_RS03870 (J8F85_03825)	gspS2	812348..812758 (+)	411	WP_001300497.1	type II secretion system pilot lipoprotein GspS-beta	-
J8F85_RS03875 (J8F85_03830)	gspC	812776..813735 (+)	960	WP_000135079.1	type II secretion system protein GspC	-
J8F85_RS22575 (J8F85_03835)	-	813846..814790 (+)	945	Protein_763	secretin N-terminal domain-containing protein	-
J8F85_RS03885 (J8F85_03840)	-	815017..815172 (+)	156	Protein_764	GspL/Epsl periplasmic domain-containing protein	-
J8F85_RS03890 (J8F85_03845)	yghD	815174..815710 (+)	537	WP_000942785.1	GspM family type II secretion system protein YghD	-
J8F85_RS03900 (J8F85_03855)	yqgA	816040..816747 (-)	708	WP_000234514.1	DUF554 domain-containing protein	-
J8F85_RS03905	yqgH	816900..816983 (+)	84	WP_211180506.1	protein YqgH	-

Sequence

Protein

Download Length: 269 a.a. Molecular weight: 29446.02 Da Isoelectric Point: 8.3829

                                >NTDB_id=484243 J8F85_RS03865 WP_001547020.1 811473..812282(+) (pilD) [Escherichia coli strain WG5::TT12A]

MLFDVFQQYPAAMPVLATVGGLIIGSFLNVVIWRYPIMLRQQMAEFHGEMPSAQSKISLALPRSHCPHCQQTIRVRDNIP

LFSWLMLKGRCRDCQAKISKRYPLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDHQWLPDVFTQGVLWT

GLSAAWAQQSPLTLQDAVTGVLVGFIAFYSLRWIAGIVLRKEALGMGDVLLFAALGSWVGPLSLPNVALIASCCGLIYAV

ITKRGSTTLPFGPCLSLGGIATIYLQALF

Nucleotide

Download Length: 810 bp

                                >NTDB_id=484243 J8F85_RS03865 WP_001547020.1 811473..812282(+) (pilD) [Escherichia coli strain WG5::TT12A]

ATGCTTTTTGATGTTTTTCAGCAATACCCTGCGGCGATGCCCGTCCTGGCAACCGTCGGAGGATTGATTATAGGTAGTTT

TTTGAATGTGGTGATTTGGCGTTACCCCATCATGCTGCGCCAACAAATGGCGGAGTTTCACGGTGAAATGCCGAGTGCGC

AGTCAAAAATAAGCCTGGCGCTGCCACGTTCGCACTGTCCACATTGTCAGCAGACCATCCGCGTTCGTGACAATATTCCG

CTGTTCTCCTGGCTGATGCTCAAAGGGCGCTGCCGCGACTGTCAGGCGAAAATCAGCAAGCGTTATCCGCTGGTGGAGTT

ATTGACGGCACTCGCTTTTTTGCTGGCGAGTCTGGTCTGGCCGGAAAGTGGATGGGCGCTGGCGGTGATGATATTATCCG

CCTGGCTGATTGCCGCGAGCGTCATTGACCTCGATCACCAATGGCTGCCCGATGTTTTTACTCAGGGCGTATTGTGGACG

GGACTGAGTGCGGCATGGGCGCAGCAGAGCCCGCTCACGCTACAAGATGCAGTTACCGGCGTCCTGGTGGGGTTTATCGC

TTTTTACTCCCTGCGCTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTATTGTTCGCTG

CGTTAGGTAGTTGGGTGGGGCCGTTGTCGCTACCCAATGTTGCTTTAATCGCATCATGCTGCGGCCTGATATATGCCGTT

ATTACAAAAAGAGGATCAACCACACTGCCTTTTGGACCGTGTTTAAGTCTGGGCGGTATAGCAACAATTTATCTACAGGC

ATTGTTTTAA

Domains

Predicted by InterProScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)

Type IV leader peptidase family (Peptidase_A24)

(130-239)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilD	Vibrio cholerae strain A1552	46.067	99.257	0.457
pilD	Vibrio campbellii strain DS40M4	42.322	99.257	0.42
pilD	Acinetobacter nosocomialis M2	38.113	98.513	0.375
pilD	Neisseria gonorrhoeae MS11	38.911	95.539	0.372
pilD	Acinetobacter baumannii D1279779	37.736	98.513	0.372

Detailed information NTG0484243