Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IC764_RS15275 Genome accession   NZ_CP061683
Coordinates   3174098..3175135 (+) Length   345 a.a.
NCBI ID   WP_019456823.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain AS5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 3120485..3208908 3174098..3175135 within 0


Gene organization within MGE regions


Location: 3120485..3208908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC764_RS15085 (IC764_15085) - 3121508..3123421 (-) 1914 WP_190977891.1 alpha/beta fold hydrolase -
  IC764_RS15090 (IC764_15090) - 3123568..3124275 (-) 708 WP_049066035.1 YoaK family protein -
  IC764_RS15095 (IC764_15095) - 3124479..3125369 (-) 891 WP_077170071.1 LysR family transcriptional regulator -
  IC764_RS15100 (IC764_15100) - 3125557..3126501 (+) 945 WP_077170070.1 dihydrodipicolinate synthase family protein -
  IC764_RS15105 (IC764_15105) - 3126675..3128060 (+) 1386 WP_077170069.1 MFS transporter -
  IC764_RS15110 (IC764_15110) - 3128138..3129484 (+) 1347 WP_151792979.1 OprD family outer membrane porin -
  IC764_RS15115 (IC764_15115) - 3130132..3130416 (+) 285 WP_151792978.1 hypothetical protein -
  IC764_RS15120 (IC764_15120) - 3130629..3131246 (+) 618 WP_014206002.1 hypothetical protein -
  IC764_RS15125 (IC764_15125) - 3131334..3131879 (+) 546 WP_190977890.1 hypothetical protein -
  IC764_RS15130 (IC764_15130) - 3131914..3133073 (+) 1160 WP_085945698.1 IS3 family transposase -
  IC764_RS15135 (IC764_15135) - 3133369..3134238 (-) 870 WP_190977889.1 hypothetical protein -
  IC764_RS15140 (IC764_15140) - 3134289..3137195 (-) 2907 WP_190977888.1 type I restriction endonuclease subunit R -
  IC764_RS15145 (IC764_15145) - 3137321..3138232 (-) 912 WP_151792537.1 DUF5655 domain-containing protein -
  IC764_RS15150 (IC764_15150) - 3138272..3139384 (-) 1113 WP_151739591.1 PD-(D/E)XK nuclease family protein -
  IC764_RS15155 (IC764_15155) - 3139495..3139953 (-) 459 WP_151739592.1 hypothetical protein -
  IC764_RS15160 (IC764_15160) - 3140025..3141188 (-) 1164 WP_190977887.1 restriction endonuclease subunit S -
  IC764_RS15165 (IC764_15165) - 3141236..3142807 (-) 1572 WP_151792539.1 type I restriction-modification system subunit M -
  IC764_RS15170 (IC764_15170) - 3143015..3143464 (+) 450 WP_151792540.1 hypothetical protein -
  IC764_RS15175 (IC764_15175) - 3143510..3144100 (-) 591 WP_151792541.1 hypothetical protein -
  IC764_RS15180 (IC764_15180) - 3144353..3144808 (-) 456 WP_151792542.1 hypothetical protein -
  IC764_RS15185 (IC764_15185) - 3145111..3151407 (-) 6297 WP_151792543.1 DEAD/DEAH box helicase -
  IC764_RS15190 (IC764_15190) - 3151409..3154777 (-) 3369 WP_151792544.1 STY4851/ECs_5259 family protein -
  IC764_RS15195 (IC764_15195) - 3155557..3155832 (+) 276 WP_151792545.1 hypothetical protein -
  IC764_RS15205 (IC764_15205) ggt 3156408..3158390 (-) 1983 WP_151792546.1 gamma-glutamyltransferase -
  IC764_RS15210 (IC764_15210) - 3158696..3160219 (-) 1524 WP_151792547.1 DHA2 family efflux MFS transporter permease subunit -
  IC764_RS15215 (IC764_15215) - 3160226..3161377 (-) 1152 WP_019456813.1 EmrA/EmrK family multidrug efflux transporter periplasmic adaptor subunit -
  IC764_RS15220 (IC764_15220) - 3161648..3162145 (-) 498 WP_004703260.1 thioesterase family protein -
  IC764_RS15225 (IC764_15225) hemB 3162288..3163301 (+) 1014 WP_151792548.1 porphobilinogen synthase -
  IC764_RS15230 (IC764_15230) - 3163327..3164442 (+) 1116 WP_151792549.1 NAD(P)/FAD-dependent oxidoreductase -
  IC764_RS15235 (IC764_15235) - 3164434..3165339 (-) 906 WP_151792550.1 TIGR01777 family oxidoreductase -
  IC764_RS15240 (IC764_15240) - 3165423..3165875 (-) 453 WP_049066316.1 ABZJ_00895 family protein -
  IC764_RS15245 (IC764_15245) - 3166250..3166627 (+) 378 WP_151792551.1 VOC family protein -
  IC764_RS15250 (IC764_15250) - 3166702..3167175 (-) 474 WP_215902048.1 OsmC family protein -
  IC764_RS15255 (IC764_15255) - 3167340..3168608 (+) 1269 WP_151792552.1 exonuclease SbcCD subunit D -
  IC764_RS15260 (IC764_15260) - 3168618..3172214 (+) 3597 WP_151792553.1 SbcC/MukB-like Walker B domain-containing protein -
  IC764_RS15265 (IC764_15265) - 3172330..3173172 (-) 843 WP_019456821.1 undecaprenyl-diphosphate phosphatase -
  IC764_RS15270 (IC764_15270) - 3173279..3173971 (-) 693 WP_077170049.1 YggS family pyridoxal phosphate-dependent enzyme -
  IC764_RS15275 (IC764_15275) pilT 3174098..3175135 (+) 1038 WP_019456823.1 type IV pilus twitching motility protein PilT Machinery gene
  IC764_RS15280 (IC764_15280) pilU 3175163..3176281 (+) 1119 WP_004703285.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IC764_RS15285 (IC764_15285) fur 3176351..3176788 (-) 438 WP_004703287.1 ferric iron uptake transcriptional regulator -
  IC764_RS15290 (IC764_15290) - 3176900..3177298 (+) 399 WP_004703289.1 outer membrane protein assembly factor BamE -
  IC764_RS15295 (IC764_15295) - 3177354..3177677 (-) 324 WP_019456825.1 RnfH family protein -
  IC764_RS15300 (IC764_15300) - 3177677..3178744 (-) 1068 WP_143221137.1 hypothetical protein -
  IC764_RS15305 (IC764_15305) - 3178897..3179352 (-) 456 WP_004703295.1 bacteriohemerythrin -
  IC764_RS15310 (IC764_15310) - 3179603..3180451 (-) 849 WP_077170048.1 class II glutamine amidotransferase -
  IC764_RS15315 (IC764_15315) - 3180560..3181342 (-) 783 WP_077170047.1 GNAT family N-acetyltransferase -
  IC764_RS15320 (IC764_15320) argB 3181461..3182369 (-) 909 WP_004703301.1 acetylglutamate kinase -
  IC764_RS15325 (IC764_15325) - 3182385..3183803 (-) 1419 WP_077170046.1 phosphomannomutase/phosphoglucomutase -
  IC764_RS15330 (IC764_15330) dut 3183999..3184451 (-) 453 WP_000868152.1 dUTP diphosphatase -
  IC764_RS15335 (IC764_15335) - 3184476..3186515 (-) 2040 WP_039254369.1 UvrD-helicase domain-containing protein -
  IC764_RS15340 (IC764_15340) - 3186709..3187362 (-) 654 WP_077170045.1 OmpA family protein -
  IC764_RS15345 (IC764_15345) - 3187608..3188534 (+) 927 WP_151792554.1 acyltransferase -
  IC764_RS15350 (IC764_15350) - 3188549..3189595 (+) 1047 WP_151792555.1 preprotein translocase subunit SecA -
  IC764_RS15355 (IC764_15355) minC 3189717..3190439 (+) 723 WP_190977886.1 septum site-determining protein MinC -
  IC764_RS15360 (IC764_15360) minD 3190510..3191322 (+) 813 WP_001074362.1 septum site-determining protein MinD -
  IC764_RS15365 (IC764_15365) minE 3191325..3191597 (+) 273 WP_000896934.1 cell division topological specificity factor MinE -
  IC764_RS15370 (IC764_15370) - 3191681..3191968 (+) 288 WP_004703320.1 PA4642 family protein -
  IC764_RS15375 (IC764_15375) rhtC 3192017..3192637 (-) 621 WP_004703321.1 threonine export protein RhtC -
  IC764_RS15380 (IC764_15380) - 3192788..3195820 (-) 3033 WP_151792557.1 DNA translocase FtsK -
  IC764_RS15385 (IC764_15385) trxB 3196090..3197037 (+) 948 WP_004703323.1 thioredoxin-disulfide reductase -
  IC764_RS15390 (IC764_15390) aat 3197157..3197888 (+) 732 WP_004703324.1 leucyl/phenylalanyl-tRNA--protein transferase -
  IC764_RS15395 (IC764_15395) - 3197907..3198722 (+) 816 WP_004703325.1 arginyltransferase -
  IC764_RS15400 (IC764_15400) rimI 3198761..3199216 (-) 456 WP_077170040.1 ribosomal protein S18-alanine N-acetyltransferase -
  IC764_RS15405 (IC764_15405) - 3199325..3200200 (-) 876 WP_019456837.1 metal-dependent hydrolase -
  IC764_RS15410 (IC764_15410) - 3200439..3201320 (-) 882 WP_077170039.1 metal-dependent hydrolase -
  IC764_RS15415 (IC764_15415) tuf 3201523..3202713 (-) 1191 WP_002157016.1 elongation factor Tu -
  IC764_RS15420 (IC764_15420) fusA 3202808..3204946 (-) 2139 WP_039255563.1 elongation factor G -
  IC764_RS15425 (IC764_15425) rpsG 3205124..3205594 (-) 471 WP_001138055.1 30S ribosomal protein S7 -
  IC764_RS15430 (IC764_15430) rpsL 3205754..3206128 (-) 375 WP_002050319.1 30S ribosomal protein S12 -
  IC764_RS15435 (IC764_15435) - 3206329..3207645 (-) 1317 WP_004703334.1 EcsC family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38372.98 Da        Isoelectric Point: 6.6653

>NTDB_id=484168 IC764_RS15275 WP_019456823.1 3174098..3175135(+) (pilT) [Acinetobacter seifertii strain AS5]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMMDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KGLVSRGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=484168 IC764_RS15275 WP_019456823.1 3174098..3175135(+) (pilT) [Acinetobacter seifertii strain AS5]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCTTCCGATTTGCATTTATCTGCTGGCATGCCACCAAT
GATTCGTGTCGATGGTGAAGTTCGCCGTATTAACTTACCAGCCTTAGAACATAAAGATGTCCACCGCCTTGTGTACGACA
TCATGAACGATAAACAGCGCCGTGATTATGAAGAAAAACTCGAAACTGACTTTTCTTTTGAAGTACCTAATGTTGCACGT
TTCCGTGTGAACGCATTTAACCAAAACCGTGGTGCTGGCGCAGTATTTCGTACCATTCCATCTAAAGTACTCACCATGGA
AGATTTAGGCTTAGGTCAAATCTTTAAAGACATTTGTGACTATCCTCGCGGGATTGTCCTAGTTACAGGTCCAACAGGTT
CGGGTAAATCAACCACGCTTGCAGCGATGATGGACTACATTAATGAAAATCGTTATGACCATATTTTAACGGTTGAAGAC
CCAATCGAATTTGTACACCAATCAAAAAAATGCCTCATTAACCAGCGTGAAGTCCATCGTGATACACATGGCTTTAATGA
AGCACTTCGCTCAGCACTGCGTGAAGATCCTGATATTATTTTGGTCGGTGAGATGCGTGACCTTGAAACGATTCGTTTGG
CCCTTACAGCTGCCGAAACAGGTCACTTAGTATTTGGTACGCTTCATACCACATCTGCCGCTAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTACGTGCCATGCTGTCTGAATCTTTGCAAGCCGTTATTTCGCAAAC
TCTGCTTAAAAAGAACGGCGGCGGTCGTGTGGCAGCACATGAAATCATGATTGGTATTCCTGCCATTCGTAACTTAATCC
GTGAAAACAAAGTCGCACAAATGTATTCAGCCATTCAAACTGGTGCTAACTATGGCATGACAACGCTTGACCAAAGCTTA
AAAGGTTTAGTGTCACGTGGTGTAATTAGCCCACAAACTGCACGTACTGCGGCAAAACAGCCTGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

99.42

100

0.994
  pilT Acinetobacter baumannii D1279779

99.13

100

0.991
  pilT Acinetobacter baumannii strain A118

99.13

100

0.991
  pilT Acinetobacter baylyi ADP1

88.081

99.71

0.878
  pilT Pseudomonas aeruginosa PAK

83.721

99.71

0.835
  pilT Pseudomonas stutzeri DSM 10701

83.43

99.71

0.832
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.131

97.391

0.693
  pilT Vibrio cholerae strain A1552

71.131

97.391

0.693
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388