Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   IC785_RS01615 Genome accession   NZ_CP061603
Coordinates   345191..345931 (+) Length   246 a.a.
NCBI ID   WP_077168923.1    Uniprot ID   A0A7H2VHI1
Organism   Acinetobacter seifertii strain AS53     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 340191..350931
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC785_RS01600 (IC785_01600) ponA 340775..343330 (-) 2556 WP_134262504.1 penicillin-binding protein PBP1a -
  IC785_RS01605 (IC785_01605) pilM 343492..344550 (+) 1059 WP_004705187.1 pilus assembly protein PilM Machinery gene
  IC785_RS01610 (IC785_01610) pilN 344550..345194 (+) 645 WP_004705190.1 PilN domain-containing protein Machinery gene
  IC785_RS01615 (IC785_01615) pilO 345191..345931 (+) 741 WP_077168923.1 type 4a pilus biogenesis protein PilO Machinery gene
  IC785_RS01620 (IC785_01620) pilP 345942..346469 (+) 528 WP_077168924.1 pilus assembly protein PilP Machinery gene
  IC785_RS01625 (IC785_01625) pilQ 346531..348657 (+) 2127 WP_151792203.1 type IV pilus secretin PilQ family protein Machinery gene
  IC785_RS01630 (IC785_01630) aroK 348669..349238 (+) 570 WP_004705195.1 shikimate kinase AroK -
  IC785_RS01635 (IC785_01635) aroB 349267..350349 (+) 1083 WP_077168926.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27889.51 Da        Isoelectric Point: 4.8393

>NTDB_id=483507 IC785_RS01615 WP_077168923.1 345191..345931(+) (pilO) [Acinetobacter seifertii strain AS53]
MSQDELQELSLEQTSSKNKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFVAIQPKLHAIDNAQAQE
SNLLNEFREKDSKLRNLRQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLENEVKQEVFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPTKDSKSDIPVLNYSIKAKTYRYVEATDTAEQSTQAPTPPASTS
TTAQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=483507 IC785_RS01615 WP_077168923.1 345191..345931(+) (pilO) [Acinetobacter seifertii strain AS53]
ATGAGTCAAGATGAACTTCAAGAATTATCATTAGAGCAAACATCTTCAAAGAACAAGAAATTTAATCTCGATAAATTCCT
TCAACAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCCATATCTGTAAAAATTACTTGTTGGATTTTTATTT
TCTTTGCTGTATTAGCTTTAGGTTATTTTGTTGCTATTCAGCCAAAGCTACACGCAATTGATAATGCTCAAGCTCAGGAA
AGTAACTTGTTAAACGAATTTCGAGAAAAAGATTCGAAATTACGTAACTTGCGGCAGTATCAAGTTCAACTTCAAGAGAT
GCAAGCAAATTTTAATCAGCAATTAGAGCAGTTGCCTAAAGAAACAGAGATTCCTAGTTTGGTTGAGGATATTAACTTAA
CTGGTGTGAACTCAGGATTGAAATTTAAAAATATTCGCTTGGAAAATGAAGTCAAACAGGAAGTCTTTATTGAACAACCG
ATTGCGATAGAAGCCACAGGTGATTATCACGCCTTTGGAGCTTTTGTGAGTAGTATTGCTGCGTTGCCGCGTATCGTAAC
CATGCATGACTTTACTGTTGATGTATCTCCAACTAAAGACAGCAAGTCTGATATTCCTGTATTGAATTATTCTATTAAAG
CGAAAACCTATCGCTATGTAGAAGCAACAGATACAGCGGAGCAATCGACCCAAGCTCCAACACCACCTGCTTCAACTAGC
ACTACTGCTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H2VHI1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

91.463

100

0.915
  comO Acinetobacter baylyi ADP1

67.347

99.593

0.671