Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ICJ55_RS04525 Genome accession   NZ_CP061280
Coordinates   881113..881670 (+) Length   185 a.a.
NCBI ID   WP_188157491.1    Uniprot ID   A0A7H1C4U0
Organism   Mannheimia bovis strain ZY190616     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 876113..886670
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ICJ55_RS04495 (ICJ55_04495) - 876286..877095 (+) 810 WP_188157486.1 metal ABC transporter permease -
  ICJ55_RS04500 (ICJ55_04500) - 877143..877886 (-) 744 WP_188157487.1 metal ABC transporter ATP-binding protein -
  ICJ55_RS04505 (ICJ55_04505) - 877898..878794 (-) 897 WP_025247305.1 metal ABC transporter substrate-binding protein -
  ICJ55_RS04510 (ICJ55_04510) - 879042..880202 (+) 1161 WP_188157488.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  ICJ55_RS04515 (ICJ55_04515) - 880240..880542 (-) 303 WP_188157489.1 helix-turn-helix transcriptional regulator -
  ICJ55_RS04520 (ICJ55_04520) - 880526..880897 (-) 372 WP_188157490.1 type II toxin-antitoxin system RelE/ParE family toxin -
  ICJ55_RS04525 (ICJ55_04525) pilF 881113..881670 (+) 558 WP_188157491.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ICJ55_RS04530 (ICJ55_04530) - 881667..884003 (+) 2337 WP_188157492.1 Tex family protein -
  ICJ55_RS04535 (ICJ55_04535) - 884083..884460 (+) 378 WP_188157493.1 NirD/YgiW/YdeI family stress tolerance protein -
  ICJ55_RS04540 (ICJ55_04540) - 884534..885355 (-) 822 WP_188157494.1 SDR family NAD(P)-dependent oxidoreductase -
  ICJ55_RS04545 (ICJ55_04545) - 885357..885920 (-) 564 WP_188157495.1 nitroreductase family protein -

Sequence

Protein


Download         Length: 185 a.a.        Molecular weight: 21162.03 Da        Isoelectric Point: 7.0809

>NTDB_id=481465 ICJ55_RS04525 WP_188157491.1 881113..881670(+) (pilF) [Mannheimia bovis strain ZY190616]
MFAKFFRKITACMAIVLLAACSSIPQPSVEPEFNRSEAVKARINLALAYLEQNDFPKAKQNIDKALAHDSKDYLPYSVLA
YYYQQTGEVENAEKAYQQALNLSENRPDVLNNYGTFLCKQGQFEQAYQQFEQAVKSPKPYYHQADSLENIILCAKKEPNP
QKLNETLTQLEKLDKARANLLKENQ

Nucleotide


Download         Length: 558 bp        

>NTDB_id=481465 ICJ55_RS04525 WP_188157491.1 881113..881670(+) (pilF) [Mannheimia bovis strain ZY190616]
ATGTTTGCAAAATTTTTTAGAAAAATAACCGCTTGTATGGCGATAGTTTTATTGGCAGCCTGTTCTTCTATCCCTCAGCC
TTCTGTTGAGCCTGAATTTAACCGTTCAGAAGCGGTGAAAGCCCGCATTAATCTTGCTTTAGCTTATTTAGAGCAAAATG
ATTTCCCCAAAGCGAAACAAAATATTGATAAAGCCCTTGCCCACGACAGCAAAGATTACCTGCCTTACTCGGTGCTTGCT
TACTATTACCAACAGACAGGCGAGGTAGAAAATGCCGAAAAAGCCTATCAACAAGCCCTCAATTTAAGTGAAAATCGTCC
TGATGTGCTGAATAATTACGGCACTTTTTTATGCAAACAAGGTCAATTTGAACAGGCATATCAACAATTTGAGCAGGCTG
TAAAAAGCCCAAAACCCTATTATCATCAAGCAGATAGCTTAGAAAATATCATTCTGTGTGCGAAAAAAGAGCCAAATCCG
CAGAAATTGAACGAAACATTAACCCAGCTCGAAAAATTAGATAAAGCTCGAGCGAATTTATTGAAGGAAAATCAATGA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(95-126)

Tetratricopeptide repeat (TPR_8)

(40-66)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H1C4U0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

63.636

100

0.643
  pilF/pilF2 Haemophilus influenzae Rd KW20

47.674

92.973

0.443