Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   SPN034183_RS00380 Genome accession   NC_021028
Coordinates   59457..60818 (+) Length   453 a.a.
NCBI ID   WP_074017595.1    Uniprot ID   A0A237IXT5
Organism   Streptococcus pneumoniae SPN034183     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 54457..65818
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPN034183_RS13370 (SPN034183_00590) - 54479..54595 (+) 117 WP_001063632.1 hypothetical protein -
  SPN034183_RS00330 (SPN034183_00600) - 54576..54779 (+) 204 WP_001091113.1 hypothetical protein -
  SPN034183_RS00335 (SPN034183_00610) - 54782..55132 (+) 351 WP_000852241.1 hypothetical protein -
  SPN034183_RS00340 (SPN034183_00620) - 55141..55557 (+) 417 WP_001165344.1 phage holin family protein -
  SPN034183_RS00345 (SPN034183_00630) - 55561..55893 (+) 333 WP_001186219.1 phage holin -
  SPN034183_RS00350 (SPN034183_00640) - 55897..56853 (+) 957 WP_000350505.1 N-acetylmuramoyl-L-alanine amidase family protein -
  SPN034183_RS00360 - 57074..57262 (-) 189 WP_000109850.1 hypothetical protein -
  SPN034183_RS00365 (SPN034183_00670) tadA 57830..58297 (+) 468 WP_000291870.1 tRNA adenosine(34) deaminase TadA -
  SPN034183_RS00370 (SPN034183_00680) - 58483..58926 (+) 444 WP_000701992.1 dUTP diphosphatase -
  SPN034183_RS00375 (SPN034183_00690) - 58928..59443 (+) 516 WP_001838385.1 histidine phosphatase family protein -
  SPN034183_RS00380 (SPN034183_00700) radA 59457..60818 (+) 1362 WP_074017595.1 DNA repair protein RadA Machinery gene
  SPN034183_RS00385 (SPN034183_00710) - 60891..61388 (+) 498 WP_001809263.1 carbonic anhydrase -
  SPN034183_RS00395 (SPN034183_00720) - 61413..62243 (+) 831 Protein_71 PrsW family glutamic-type intramembrane protease -
  SPN034183_RS00400 (SPN034183_00730) - 62388..63356 (+) 969 WP_000010163.1 ribose-phosphate diphosphokinase -
  SPN034183_RS11075 (SPN034183_00740) - 63493..63771 (-) 279 Protein_73 transposase family protein -
  SPN034183_RS12875 - 63815..64740 (-) 926 Protein_74 Rpn family recombination-promoting nuclease/putative transposase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49432.95 Da        Isoelectric Point: 6.5137

>NTDB_id=48130 SPN034183_RS00380 WP_074017595.1 59457..60818(+) (radA) [Streptococcus pneumoniae SPN034183]
MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=48130 SPN034183_RS00380 WP_074017595.1 59457..60818(+) (radA) [Streptococcus pneumoniae SPN034183]
ATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGCCCCAACTG
TGGGTCTTGGTCTTCTTTTGTGGAAGAGGTTGAGGTTGCCGAGGTCAAGAATGCGCGTGTGTCCTTGACAGGTGAGAAAA
CCAAGCCCATGAAACTAGCTGAGGTGACTTCCATCAATGTCAATCGAACCAAGACGGAGATGGAGGAATTCAACCGTGTG
CTTGGAGGCGGAGTTGTACCAGGAAGTCTCGTCCTCATCGGTGGGGATCCTGGGATTGGGAAATCAACTCTTCTCCTACA
AGTCTCAACCCAGTTGTCCCAAGTGGGGACAGTTCTCTATGTCAGTGGGGAGGAGTCTGCCCAGCAGATTAAACTACGTG
CAGAGCGCTTAGGTGATATTGATAGTGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGTGCAGAAGTGGAG
CGTATCCAGCCAGACTTTCTCATTATTGATTCCATCCAGACCATCATGTCTCCTGAGATTTCAGGGGTGCAGGGGTCTGT
TTCTCAGGTGCGTGAAGTGACCGCTGAACTCATGCAGTTGGCCAAGACCAATAACATTGCCATCTTTATCGTAGGTCATG
TGACCAAAGAAGGAACCTTGGCTGGGCCTCGTATGTTGGAGCATATGGTGGATACGGTGCTTTACTTTGAAGGGGAGCGT
CACCACACCTTTCGTATTTTGAGAGCGGTCAAAAATCGTTTTGGTTCCACTAATGAGATTGGGATTTTTGAGATGCAGTC
GGGCGGCTTGGTTGAGGTACTCAATCCGAGTCAAGTTTTCCTAGAAGAGCGTTTGGATGGGGCGACTGGTTCCTCCATCG
TTGTAACCATGGAAGGGACGCGTCCGATTTTGGCGGAGGTTCAGGCTTTGGTAACACCGACCATGTTTGGAAATGCCAAG
CGTACTACGACAGGACTTGATTTTAACCGTGCTAGCTTGATTATGGCTGTTTTGGAAAAACGGGCAGGGCTTCTCTTACA
AAATCAGGATGCCTATCTCAAATCTGCTGGTGGTGTTAAATTGGATGAACCTGCCATTGACTTGGCTGTTGCAGTTGCTA
TTGCTTCGAGCTACAAAGACAAGCCAACTAATCCTCAGGAATGTTTTGTCGGAGAACTGGGCTTGACAGGAGAGATTCGG
CGCGTGAATCGTATTGAGCAACGCATCAACGAAGCTGCTAAACTGGGCTTTACTAAGATTTATGTACCTAAGAATTCCTT
GACAGGAATCACTCTGCCTAAGGAAATTCAGGTCATTGGCGTGACAACGATTCAGGAAGTTTTGAAAAAGGTCTTTGCAT
AA

Domains

Predicted by InterProScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(63-218)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 8RXC

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

100

100

1
  radA Streptococcus pneumoniae D39

100

100

1
  radA Streptococcus pneumoniae R6

100

100

1
  radA Streptococcus pneumoniae TIGR4

100

100

1
  radA Streptococcus mitis NCTC 12261

99.558

100

0.996
  radA Streptococcus mitis SK321

99.558

100

0.996
  radA/sms Bacillus subtilis subsp. subtilis str. 168

63.135

100

0.631

Multiple sequence alignment