Detailed information NTG0047975

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: NMBM01240149_RS09900
• Genome accession: NC_017514
• Coordinates: 1926450..1927310 (+)
• Length: 286 a.a.
• NCBI ID: WP_002241067.1
• Uniprot ID: A1KVV2
• Organism: Neisseria meningitidis M01-240149
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1921450..1932310

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NMBM01240149_RS09885 (NMBM01240149_1749)	dapD	1921960..1922781 (-)	822	WP_002255671.1	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase	-
NMBM01240149_RS13345	-	1922791..1923021 (-)	231	WP_002212338.1	hypothetical protein	-
NMBM01240149_RS09890 (NMBM01240149_1750)	pgi	1922991..1924634 (-)	1644	WP_014581240.1	glucose-6-phosphate isomerase	-
NMBM01240149_RS09895 (NMBM01240149_1751)	pilG	1925144..1926376 (+)	1233	WP_002216405.1	type 4 pilus assembly protein PilG	Machinery gene
NMBM01240149_RS09900 (NMBM01240149_1752)	pilD	1926450..1927310 (+)	861	WP_002241067.1	prepilin peptidase	Machinery gene
NMBM01240149_RS09905 (NMBM01240149_1753)	coaE	1927312..1927944 (+)	633	WP_002216402.1	dephospho-CoA kinase	-
NMBM01240149_RS09910 (NMBM01240149_1754)	-	1927937..1928146 (+)	210	WP_002216401.1	DNA gyrase inhibitor YacG	-
NMBM01240149_RS09915 (NMBM01240149_1755)	pilF	1928231..1929907 (-)	1677	WP_002221967.1	type IV-A pilus assembly ATPase PilB	Machinery gene
NMBM01240149_RS15920	-	1930047..1930109 (-)	63	Protein_1847	transposase	-
NMBM01240149_RS09925 (NMBM01240149_1757)	-	1930398..1930844 (-)	447	WP_002220271.1	DUF441 domain-containing protein	-
NMBM01240149_RS09930 (NMBM01240149_1758)	ispB	1930849..1931823 (-)	975	WP_002221965.1	octaprenyl diphosphate synthase	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 31331.48 Da
• Isoelectric Point: 9.1920

>NTDB_id=47975 NMBM01240149_RS09900 WP_002241067.1 1926450..1927310(+) (pilD) [Neisseria meningitidis M01-240149]
MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVFAKKHLNLPLTEKESRTFNLMKPDSCCPKCRVPIRAW
QNIPIVSYLLLRGKCASCQTKISIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAALGAWLGISALPVLIFVSSLIGLV
AAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRAVNWWLTHPVL

Nucleotide

• Length: 861 bp

>NTDB_id=47975 NMBM01240149_RS09900 WP_002241067.1 1926450..1927310(+) (pilD) [Neisseria meningitidis M01-240149]
ATGTCTGATTTGTCTGTATTGTCGCCGTTTGCCGTGCCTTTGGCAGCGGTGTTCGGGCTGCTGGTCGGCAGTTTCTTAAA
CGTCGTCATTTACCGCGTGCCGGTCATGATGGAACGCGGCTGGACGGTATTTGCCAAAAAACATTTAAACCTGCCGCTGA
CCGAAAAGGAAAGCCGTACCTTCAACCTGATGAAACCGGATTCCTGCTGTCCCAAATGCCGCGTGCCGATACGCGCGTGG
CAGAACATCCCGATTGTCAGCTACCTGCTCCTGCGCGGCAAATGCGCTTCCTGCCAAACCAAAATCAGCATACGTTATCC
CTTAATCGAGCTGCTGACCGGCGTATTATTCGGGCTGGTCGCCTGGCAATACGGCTGGTCTTGGATTACATTGGGCGGTT
TGATACTGACCGCGTTTCTGATTTCCCTGACCTTTATCGATGCGGACACCCAATACCTGCCCGACTCGATGACACTGCCC
TTGATTTGGCTGGGGCTGATATTTAATTTGGACGGCGGCTTCGTGCCTTTGCAGTCTGCCGTTTTAGGTGCGGTTGCCGG
CTATGGTTCGATATGGCTTTTATGCGCGGTATATAAACTGATTAAAGGCGAAATCGGTATGGGCAACGGAGATTTCAAAC
TGATTGCCGCATTGGGCGCGTGGCTCGGCATATCCGCATTGCCCGTGCTGATTTTTGTTTCCTCGCTGATCGGTTTGGTC
GCGGCAATCGTTATGCGCGTCGCCAAGGGGCAGCATTTTGCCTTCGGCCCCGCACTGACAGTTTCGGGCTGGATAATTTT
TACGGCAAACGATTCCGTATGGCGGGCGGTCAACTGGTGGCTGACCCATCCGGTGCTGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (134-242)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

3D structure

• AlphaFold DB: A1KVV2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Neisseria gonorrhoeae MS11	96.14	99.65	0.958
pilD	Vibrio cholerae strain A1552	44.444	100	0.448
pilD	Acinetobacter baumannii D1279779	46.97	92.308	0.434
pilD	Acinetobacter nosocomialis M2	46.97	92.308	0.434
pilD	Vibrio campbellii strain DS40M4	44.565	96.503	0.43